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3d confocal microscopy lsm 780  (Carl Zeiss)


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    Structured Review

    Carl Zeiss 3d confocal microscopy lsm 780
    Minoxidil inhibits sarcoma cell migration and matrix remodeling in hypoxic hydrogel. KIA-GFP cells were tracked on day 3 in hypoxic-treated and untreated hydrogel to determine <t>3D</t> trajectories of tracked cells (representative trajectories) (A); overall speed (B); velocity in the x, y, and z directions (C); and MSD in the x, y, and z directions (D). Plots were created using the position of KIA-GFP cells in the hydrogels. (E) Collagen deposition and quantification (collagen in red, nuclei in blue). (Scale bars: 50 μm.) (F) Proteolytic degradation of HI hydrogels incorporating DQ Gtn for 3 d. (Left) Representative fluorescence <t>microscopy</t> images. (Scale bars: 20 μm). (Right) Quantitative analysis of relative fluorescence intensity. (G) Western blot analyses for PLOD2 in KIA cells cultured in hypoxic conditions with and without minoxidil treatment. Significance levels: *P < 0.05; ^P < 0.01; #P < 0.001.
    3d Confocal Microscopy Lsm 780, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/3d+cell+tracking+algorithm/780+confocal+lsm+microscope/pmc04995943-293-7-13
    Average 90 stars, based on 1 article reviews
    3d confocal microscopy lsm 780 - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Intratumoral oxygen gradients mediate sarcoma cell invasion"

    Article Title: Intratumoral oxygen gradients mediate sarcoma cell invasion

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1605317113

    Minoxidil inhibits sarcoma cell migration and matrix remodeling in hypoxic hydrogel. KIA-GFP cells were tracked on day 3 in hypoxic-treated and untreated hydrogel to determine 3D trajectories of tracked cells (representative trajectories) (A); overall speed (B); velocity in the x, y, and z directions (C); and MSD in the x, y, and z directions (D). Plots were created using the position of KIA-GFP cells in the hydrogels. (E) Collagen deposition and quantification (collagen in red, nuclei in blue). (Scale bars: 50 μm.) (F) Proteolytic degradation of HI hydrogels incorporating DQ Gtn for 3 d. (Left) Representative fluorescence microscopy images. (Scale bars: 20 μm). (Right) Quantitative analysis of relative fluorescence intensity. (G) Western blot analyses for PLOD2 in KIA cells cultured in hypoxic conditions with and without minoxidil treatment. Significance levels: *P < 0.05; ^P < 0.01; #P < 0.001.
    Figure Legend Snippet: Minoxidil inhibits sarcoma cell migration and matrix remodeling in hypoxic hydrogel. KIA-GFP cells were tracked on day 3 in hypoxic-treated and untreated hydrogel to determine 3D trajectories of tracked cells (representative trajectories) (A); overall speed (B); velocity in the x, y, and z directions (C); and MSD in the x, y, and z directions (D). Plots were created using the position of KIA-GFP cells in the hydrogels. (E) Collagen deposition and quantification (collagen in red, nuclei in blue). (Scale bars: 50 μm.) (F) Proteolytic degradation of HI hydrogels incorporating DQ Gtn for 3 d. (Left) Representative fluorescence microscopy images. (Scale bars: 20 μm). (Right) Quantitative analysis of relative fluorescence intensity. (G) Western blot analyses for PLOD2 in KIA cells cultured in hypoxic conditions with and without minoxidil treatment. Significance levels: *P < 0.05; ^P < 0.01; #P < 0.001.

    Techniques Used: Migration, Fluorescence, Microscopy, Western Blot, Cell Culture

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    Confocal:

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    Article Snippet: Fluorescence microscope AxioObserver 20× objective, Axiocam 503 mono, filter cube for red dyes , Zeiss , www.zeiss.com. .. Confocal laser scanning microscope LSM 780 , Zeiss , www.zeiss.com. .. NEX5 digital camera with a SONY macro E3.5/30 objective , Sony, Japan , www.sony.com.

    Article Title: The Mgv1–Rlm1 axis orchestrates SAGA and SWI/SNF complexes at target promoters
    Article Snippet: .. Each strain was cultured in CM at 25°C for 16 h or in TBI at 28°C for 24 h. The Zeiss LSM780 confocal microscopy (Göttingen, Niedersachsen, Germany) was used to observe fluorescent intensity and localization of tagged proteins. ..

    Article Title: Multifunctional hydrogel encapsulated with baicalin for full-layer regeneration of drug-resistant bacteria-infected wounds after radiotherapy
    Article Snippet: .. All the fluorescence images were collected using a Zeiss LSM 780 Laser Scanning Confocal Microscopy. .. All the histological and immunohistochemical (IHC) images of mice were taken by a Nikon Eclipse Ci-E/Ci-L/Ci-S microscope.

    Laser-Scanning Microscopy:

    Article Title: Protocol for monitoring of neutralizing antibodies to various receptor-binding domains using the tANCHOR system
    Article Snippet: Fluorescence microscope AxioObserver 20× objective, Axiocam 503 mono, filter cube for red dyes , Zeiss , www.zeiss.com. .. Confocal laser scanning microscope LSM 780 , Zeiss , www.zeiss.com. .. NEX5 digital camera with a SONY macro E3.5/30 objective , Sony, Japan , www.sony.com.

    Article Title: Cellulose nanofiber/beeswax based bigels: study of phase inversion and regulatory properties
    Article Snippet: In this study, bigels with different oleogel-hydrogel ratios were prepared using 2.0 wt% CNF hydrogel and 8.0 wt % beeswax oleogel.. Findings indicate that an oleogel-hydrogel ratio below 5:5 yields an O/W phase, whereas a ratio above 5:5 initiates phase inversion to a W/O phase.. At a 5:5 ratio, the bigel exhibit a biphasic system.

    Inverted Microscopy:

    Article Title: Ganglioglioma-induced animal model and a method for diagnosing and treating ganglioglioma and related diseases
    Article Snippet: DAPI comprised in a mounting solution (P36931, Life technology) was used for nuclear staining. .. Images were obtained using Leica DMI3000 B inverted microscope or Zeiss LSM780 confocal microscope. ..

    Microscopy:

    Article Title: Ganglioglioma-induced animal model and a method for diagnosing and treating ganglioglioma and related diseases
    Article Snippet: DAPI comprised in a mounting solution (P36931, Life technology) was used for nuclear staining. .. Images were obtained using Leica DMI3000 B inverted microscope or Zeiss LSM780 confocal microscope. ..

    Article Title: The Mgv1–Rlm1 axis orchestrates SAGA and SWI/SNF complexes at target promoters
    Article Snippet: .. Each strain was cultured in CM at 25°C for 16 h or in TBI at 28°C for 24 h. The Zeiss LSM780 confocal microscopy (Göttingen, Niedersachsen, Germany) was used to observe fluorescent intensity and localization of tagged proteins. ..

    Article Title: Multifunctional hydrogel encapsulated with baicalin for full-layer regeneration of drug-resistant bacteria-infected wounds after radiotherapy
    Article Snippet: .. All the fluorescence images were collected using a Zeiss LSM 780 Laser Scanning Confocal Microscopy. .. All the histological and immunohistochemical (IHC) images of mice were taken by a Nikon Eclipse Ci-E/Ci-L/Ci-S microscope.

    Article Title: Protocol for monitoring of neutralizing antibodies to various receptor-binding domains using the tANCHOR system
    Article Snippet: .. Verify HeLa cell transfection and cell adherence by examining mCherry fluorescence under a fluorescence microscope (e.g., Zeiss Axio Observer) and optional with a confocal laser microscope (e.g., Zeiss LSM780), see and for ref. , , , , , Subcellular localization of tANCHORed RBD in transfected HeLa cells (A) Epifluorescence image showing tANCHORed RBD fused to mCherry after fixation in a 96-well plate (Zeiss Axio Observer). (B) Confocal laser scanning microscopy (CLSM) image showing membrane-localized RBD expression (Zeiss LSM780). ..

    other:

    Article Title: Computational discovery of conserved RNA structures and functional characterization of a structured lncRNA in Leishmania braziliensis
    Article Snippet: Cells were imaged in a LSM 780/LSM 7 MP Carl Zeiss multiphoton microscope with 0.75 magnification.

    Article Title: The STING pathway drives noninflammatory neurodegeneration in NGLY1 deficiency
    Article Snippet: Fluorophore-conjugated secondary antibodies were used as follows: Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (cat #A32731, 1:200 dilution; Thermo Fisher Scientific); Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 546 (cat #A10036, 1:200 dilution; Thermo Fisher Scientific); Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 (cat #A-31571, 1:500 dilution; Thermo Fisher Scientific).

    Cell Culture:

    Article Title: The Mgv1–Rlm1 axis orchestrates SAGA and SWI/SNF complexes at target promoters
    Article Snippet: .. Each strain was cultured in CM at 25°C for 16 h or in TBI at 28°C for 24 h. The Zeiss LSM780 confocal microscopy (Göttingen, Niedersachsen, Germany) was used to observe fluorescent intensity and localization of tagged proteins. ..

    Confocal Laser Scanning Microscopy:

    Article Title: Cellulose nanofiber/beeswax based bigels: study of phase inversion and regulatory properties
    Article Snippet: In this study, bigels with different oleogel-hydrogel ratios were prepared using 2.0 wt% CNF hydrogel and 8.0 wt % beeswax oleogel.. Findings indicate that an oleogel-hydrogel ratio below 5:5 yields an O/W phase, whereas a ratio above 5:5 initiates phase inversion to a W/O phase.. At a 5:5 ratio, the bigel exhibit a biphasic system.

    Article Title: Protocol for monitoring of neutralizing antibodies to various receptor-binding domains using the tANCHOR system
    Article Snippet: .. Verify HeLa cell transfection and cell adherence by examining mCherry fluorescence under a fluorescence microscope (e.g., Zeiss Axio Observer) and optional with a confocal laser microscope (e.g., Zeiss LSM780), see and for ref. , , , , , Subcellular localization of tANCHORed RBD in transfected HeLa cells (A) Epifluorescence image showing tANCHORed RBD fused to mCherry after fixation in a 96-well plate (Zeiss Axio Observer). (B) Confocal laser scanning microscopy (CLSM) image showing membrane-localized RBD expression (Zeiss LSM780). ..

    Fluorescence:

    Article Title: Multifunctional hydrogel encapsulated with baicalin for full-layer regeneration of drug-resistant bacteria-infected wounds after radiotherapy
    Article Snippet: .. All the fluorescence images were collected using a Zeiss LSM 780 Laser Scanning Confocal Microscopy. .. All the histological and immunohistochemical (IHC) images of mice were taken by a Nikon Eclipse Ci-E/Ci-L/Ci-S microscope.

    Article Title: Protocol for monitoring of neutralizing antibodies to various receptor-binding domains using the tANCHOR system
    Article Snippet: .. Verify HeLa cell transfection and cell adherence by examining mCherry fluorescence under a fluorescence microscope (e.g., Zeiss Axio Observer) and optional with a confocal laser microscope (e.g., Zeiss LSM780), see and for ref. , , , , , Subcellular localization of tANCHORed RBD in transfected HeLa cells (A) Epifluorescence image showing tANCHORed RBD fused to mCherry after fixation in a 96-well plate (Zeiss Axio Observer). (B) Confocal laser scanning microscopy (CLSM) image showing membrane-localized RBD expression (Zeiss LSM780). ..

    Transfection:

    Article Title: Protocol for monitoring of neutralizing antibodies to various receptor-binding domains using the tANCHOR system
    Article Snippet: .. Verify HeLa cell transfection and cell adherence by examining mCherry fluorescence under a fluorescence microscope (e.g., Zeiss Axio Observer) and optional with a confocal laser microscope (e.g., Zeiss LSM780), see and for ref. , , , , , Subcellular localization of tANCHORed RBD in transfected HeLa cells (A) Epifluorescence image showing tANCHORed RBD fused to mCherry after fixation in a 96-well plate (Zeiss Axio Observer). (B) Confocal laser scanning microscopy (CLSM) image showing membrane-localized RBD expression (Zeiss LSM780). ..

    Membrane:

    Article Title: Protocol for monitoring of neutralizing antibodies to various receptor-binding domains using the tANCHOR system
    Article Snippet: .. Verify HeLa cell transfection and cell adherence by examining mCherry fluorescence under a fluorescence microscope (e.g., Zeiss Axio Observer) and optional with a confocal laser microscope (e.g., Zeiss LSM780), see and for ref. , , , , , Subcellular localization of tANCHORed RBD in transfected HeLa cells (A) Epifluorescence image showing tANCHORed RBD fused to mCherry after fixation in a 96-well plate (Zeiss Axio Observer). (B) Confocal laser scanning microscopy (CLSM) image showing membrane-localized RBD expression (Zeiss LSM780). ..

    Expressing:

    Article Title: Protocol for monitoring of neutralizing antibodies to various receptor-binding domains using the tANCHOR system
    Article Snippet: .. Verify HeLa cell transfection and cell adherence by examining mCherry fluorescence under a fluorescence microscope (e.g., Zeiss Axio Observer) and optional with a confocal laser microscope (e.g., Zeiss LSM780), see and for ref. , , , , , Subcellular localization of tANCHORed RBD in transfected HeLa cells (A) Epifluorescence image showing tANCHORed RBD fused to mCherry after fixation in a 96-well plate (Zeiss Axio Observer). (B) Confocal laser scanning microscopy (CLSM) image showing membrane-localized RBD expression (Zeiss LSM780). ..



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