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3d-aldehyde beads  (PolyAn GmbH)


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    Structured Review

    PolyAn GmbH 3d-aldehyde beads
    3d Aldehyde Beads, supplied by PolyAn GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2d+functionality/2d+aldehyde+functionalized+glass+surfaces+1+cm2/pmc09391071-42-26-28
    Average 90 stars, based on 1 article reviews
    3d-aldehyde beads - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Proline isomerization modulates the bacterial IsdB/hemoglobin interaction: an atomic force spectroscopy study.
    Article Snippet: Aldehyde-functionalized glass surfaces, 1 cm2 (PolyAn GmbH, Germany), were incubated with 50 μL of 10 μM Hb solutions in Buffer W for 4 h at 25 °C in an air-tight container for covalent binding between silane and protein amino groups.

    Article Title: Targeting the glycine-rich domain of TDP-43 with antibodies prevents its aggregation in vitro and reduces neurofilament levels in vivo.
    Article Snippet: Briefly, recombinant TDP-43-MBP-His6 fusion protein was captured to 3D-Aldehyde beads (PolyAN, Germany) and incubated for 90 min with hybridoma supernatant and Atto 488-coupled isotype-specific monoclonal rat antimouse IgG secondary antibodies.

    Article Title: Nanoporous, Gas Permeable PEGDA Ink for 3D Printing Organ‐on‐a‐Chip Devices
    Article Snippet: Protein Microarray for CXCL12 Quantification: The CXCL12 antibody microarray was fabricated onto 2D-Aldehyde glass slides (PolyAn, Germany) using a sciFLEXARRAYER SX microarray spotter (Scienion) equipped with a single piezo dispense capillary nozzle with coating 1 (Scienion).

    Article Title: Proline isomerization modulates the bacterial IsdB/hemoglobin interaction: an atomic force spectroscopy study
    Article Snippet: Briefly, silicon nitride AFM tips with a nominal spring constant, k nom , of 0.06 N/m, (cantilever D, SNL-10; Bruker Corporation, Massachusetts, USA), were irradiated with UV for 30 min and incubated with a solution of 2% (v/v) 3-mercatopropyl-trimethoxysilane (MPTMS) (Sigma–Aldrich Co.) in toluene (99.5%, Sigma–Aldrich Co.).

    Article Title: A Bioactive Synthetic Outer-Core Oligosaccharide Derived from a Klebsiella pneumonia Lipopolysaccharide for Bacteria Recognition.
    Article Snippet: There is an urgent need for new treatment options for carbapenem-resistant Klebsiella pneumoniae (K. pneumoniae), which is a common cause of life-threatening hospitaland community-acquired infections.. Prophylactic or therapeutic vaccination may offer an approach to control these infections, however, none has yet been approved for human use.. Here, we report the chemical synthesis of an outer core tetraand pentasaccharide derived from the lipopolysaccharide of K. pneumoniae.

    Article Title: Targeting the glycine-rich domain of TDP-43 with antibodies prevents its aggregation in vitro and reduces neurofilament levels in vivo
    Article Snippet: Hybridoma supernatants were screened 10 days later in a flow cytometry assay (iQue, Intellicyt, Sartorius, Germany).

    Article Title: Nanoscale dynamical investigation of the hemoglobin complex with the bacterial protein IsdB: is their interaction stabilized by catch bonds?
    Article Snippet: Subsequently, the silanized tips were immersed in a 1 mM solution of N-hydroxysuccinimide–polyethyleneglycol–maleimide (NHS–PEG–MAL, 3.4 kDa, hereafter PEG) (Iris Biotech, Germany) in dimethylsulfoxide (DMSO) (99.9%, Sigma–Aldrich Co.) for 3 hours at room temperature, allowing –MAL groups of the flexible PEG cross-linker to react with thiol groups of MPTMS.

    Binding Assay:

    Article Title: Proline isomerization modulates the bacterial IsdB/hemoglobin interaction: an atomic force spectroscopy study.
    Article Snippet: Aldehyde-functionalized glass surfaces, 1 cm2 (PolyAn GmbH, Germany), were incubated with 50 μL of 10 μM Hb solutions in Buffer W for 4 h at 25 °C in an air-tight container for covalent binding between silane and protein amino groups.

    Article Title: Targeting the glycine-rich domain of TDP-43 with antibodies prevents its aggregation in vitro and reduces neurofilament levels in vivo.
    Article Snippet: Briefly, recombinant TDP-43-MBP-His6 fusion protein was captured to 3D-Aldehyde beads (PolyAN, Germany) and incubated for 90 min with hybridoma supernatant and Atto 488-coupled isotype-specific monoclonal rat antimouse IgG secondary antibodies.

    Article Title: Nanoporous, Gas Permeable PEGDA Ink for 3D Printing Organ‐on‐a‐Chip Devices
    Article Snippet: Protein Microarray for CXCL12 Quantification: The CXCL12 antibody microarray was fabricated onto 2D-Aldehyde glass slides (PolyAn, Germany) using a sciFLEXARRAYER SX microarray spotter (Scienion) equipped with a single piezo dispense capillary nozzle with coating 1 (Scienion).

    Article Title: Proline isomerization modulates the bacterial IsdB/hemoglobin interaction: an atomic force spectroscopy study
    Article Snippet: Briefly, silicon nitride AFM tips with a nominal spring constant, k nom , of 0.06 N/m, (cantilever D, SNL-10; Bruker Corporation, Massachusetts, USA), were irradiated with UV for 30 min and incubated with a solution of 2% (v/v) 3-mercatopropyl-trimethoxysilane (MPTMS) (Sigma–Aldrich Co.) in toluene (99.5%, Sigma–Aldrich Co.).

    Article Title: A Bioactive Synthetic Outer-Core Oligosaccharide Derived from a Klebsiella pneumonia Lipopolysaccharide for Bacteria Recognition.
    Article Snippet: There is an urgent need for new treatment options for carbapenem-resistant Klebsiella pneumoniae (K. pneumoniae), which is a common cause of life-threatening hospitaland community-acquired infections.. Prophylactic or therapeutic vaccination may offer an approach to control these infections, however, none has yet been approved for human use.. Here, we report the chemical synthesis of an outer core tetraand pentasaccharide derived from the lipopolysaccharide of K. pneumoniae.

    Article Title: Targeting the glycine-rich domain of TDP-43 with antibodies prevents its aggregation in vitro and reduces neurofilament levels in vivo
    Article Snippet: Hybridoma supernatants were screened 10 days later in a flow cytometry assay (iQue, Intellicyt, Sartorius, Germany).

    Article Title: Nanoscale dynamical investigation of the hemoglobin complex with the bacterial protein IsdB: is their interaction stabilized by catch bonds?
    Article Snippet: Subsequently, the silanized tips were immersed in a 1 mM solution of N-hydroxysuccinimide–polyethyleneglycol–maleimide (NHS–PEG–MAL, 3.4 kDa, hereafter PEG) (Iris Biotech, Germany) in dimethylsulfoxide (DMSO) (99.9%, Sigma–Aldrich Co.) for 3 hours at room temperature, allowing –MAL groups of the flexible PEG cross-linker to react with thiol groups of MPTMS.

    Recombinant:

    Article Title: Proline isomerization modulates the bacterial IsdB/hemoglobin interaction: an atomic force spectroscopy study.
    Article Snippet: Aldehyde-functionalized glass surfaces, 1 cm2 (PolyAn GmbH, Germany), were incubated with 50 μL of 10 μM Hb solutions in Buffer W for 4 h at 25 °C in an air-tight container for covalent binding between silane and protein amino groups.

    Article Title: Targeting the glycine-rich domain of TDP-43 with antibodies prevents its aggregation in vitro and reduces neurofilament levels in vivo.
    Article Snippet: Briefly, recombinant TDP-43-MBP-His6 fusion protein was captured to 3D-Aldehyde beads (PolyAN, Germany) and incubated for 90 min with hybridoma supernatant and Atto 488-coupled isotype-specific monoclonal rat antimouse IgG secondary antibodies.

    Article Title: Nanoporous, Gas Permeable PEGDA Ink for 3D Printing Organ‐on‐a‐Chip Devices
    Article Snippet: Protein Microarray for CXCL12 Quantification: The CXCL12 antibody microarray was fabricated onto 2D-Aldehyde glass slides (PolyAn, Germany) using a sciFLEXARRAYER SX microarray spotter (Scienion) equipped with a single piezo dispense capillary nozzle with coating 1 (Scienion).

    Article Title: Proline isomerization modulates the bacterial IsdB/hemoglobin interaction: an atomic force spectroscopy study
    Article Snippet: Briefly, silicon nitride AFM tips with a nominal spring constant, k nom , of 0.06 N/m, (cantilever D, SNL-10; Bruker Corporation, Massachusetts, USA), were irradiated with UV for 30 min and incubated with a solution of 2% (v/v) 3-mercatopropyl-trimethoxysilane (MPTMS) (Sigma–Aldrich Co.) in toluene (99.5%, Sigma–Aldrich Co.).

    Article Title: A Bioactive Synthetic Outer-Core Oligosaccharide Derived from a Klebsiella pneumonia Lipopolysaccharide for Bacteria Recognition.
    Article Snippet: There is an urgent need for new treatment options for carbapenem-resistant Klebsiella pneumoniae (K. pneumoniae), which is a common cause of life-threatening hospitaland community-acquired infections.. Prophylactic or therapeutic vaccination may offer an approach to control these infections, however, none has yet been approved for human use.. Here, we report the chemical synthesis of an outer core tetraand pentasaccharide derived from the lipopolysaccharide of K. pneumoniae.

    Article Title: Targeting the glycine-rich domain of TDP-43 with antibodies prevents its aggregation in vitro and reduces neurofilament levels in vivo
    Article Snippet: Hybridoma supernatants were screened 10 days later in a flow cytometry assay (iQue, Intellicyt, Sartorius, Germany).

    Article Title: Nanoscale dynamical investigation of the hemoglobin complex with the bacterial protein IsdB: is their interaction stabilized by catch bonds?
    Article Snippet: Subsequently, the silanized tips were immersed in a 1 mM solution of N-hydroxysuccinimide–polyethyleneglycol–maleimide (NHS–PEG–MAL, 3.4 kDa, hereafter PEG) (Iris Biotech, Germany) in dimethylsulfoxide (DMSO) (99.9%, Sigma–Aldrich Co.) for 3 hours at room temperature, allowing –MAL groups of the flexible PEG cross-linker to react with thiol groups of MPTMS.



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