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recombinant murine tgf β soluble receptor ii  (R&D Systems)


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    R&D Systems recombinant murine tgf β soluble receptor ii
    Recombinant Murine Tgf β Soluble Receptor Ii, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/241-R2/Recombinant+Human+TGF-beta+RII+Protein/pmc01941925-84-11-26
    Average 94 stars, based on 23 article reviews
    recombinant murine tgf β soluble receptor ii - by Bioz Stars, 2026-09
    94/100 stars

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    Recombinant:

    Article Title: Activation of latent transforming growth factor-β1, a conserved function for pregnancy-specific beta 1-glycoproteins
    Article Snippet: In the cell-free system assay, proteins were incubated with 50 ng/ml of small latent complex (SLC) (R&D Systems) at 37°C for 1 h in a final volume of 0.1 ml of PBS in siliconized tubes. .. The samples were then transferred to wells of a 96-well Nunc Maxisorb plate that had been coated overnight with recombinant human TGF-β receptor II-Fc (R&D systems) and blocked with PBS-1%BSA. .. After 2 h, the plates were washed, and active TGF-β1 was detected with a biotinylated-antibody specific for mature TGF-β1 (BAF240, R&D systems).

    Article Title: Pregnancy specific glycoprotein 1 (PSG1) activates TGF-β and prevents dextran sodium sulfate (DSS)-induced colitis in mice
    Article Snippet: .. The samples were transferred to blocked wells of Nunc Maxisorb plates that had been coated with capture antibody that recognizes all three TGF-β isoforms or with 1.5μg/ml of recombinant human TGF-β RII-Fc (R&D systems). ..

    Article Title: Inhibition of phosphoantigen-mediated ?? T-cell proliferation by CD4 + CD25 + FoxP3 + regulatory T cells
    Article Snippet: .. The following compounds, monoclonal antibodies (mAbs), assays and kits were used in this study: BrHPP (kindly provided by Innate Pharma, Marseilles, France), recombinant human IL-2 (Proleukin; Novartis, Basel, Switzerland), the anti-human FoxP3 Staining Kit (eBiosciences, San Diego, CA), interferon (IFN)-γ secretion assay, CD4 + CD25 + Treg isolation kit and anti-PE microbeads (all from Miltenyi Biotec, Bergisch Gladbach, Germany), recombinant human (rh) cytomegalovirus (CMV) pp65 protein (Miltenyi Biotec) 1-methyl- l -tryptophan, phytohaemagglutinin (PHA), the PKH26 red fluorescent cell linker mini kit (Sigma-Aldrich, Taufkirchen, Germany), anti-TGF-β mAb, recombinant human TGF-β soluble receptor II (sRII), anti-IL-10 mAb, human latency-associated petide (LAP; R&D Systems, Wiesbaden, Germany), proteinase K (Merck, Darmstadt, Germany), ToPro-3 iodide, the Vybrant®CFDA SE cell tracer kit (Molecular Probes, Eugene, OR) granulocyte–macrophage colony-stimulating factor (GM-CSF), IL-4, IL-15, IL-1β, IL-6, TNF-α (Tebu-Bio, Offenbach, Germany), prostaglandin E2 (Biomol, Hamburg, Germany), iTAg Tetramer/PE-HLA-A*0201 CMV pp65 (NLVPMVATV; Beckmann Coulter Immunomics Operations, Villepinte Roissy, France), N G -monomethyl- l -arginine ( l -NMMA) and N ω -hydroxy-nor- l -arginine (nor-NOHA; Axxora, Grünberg, Germany). .. Cells were harvested after the indicated * culture periods and analysed by two- or three-colour flow cytometry (FACScan flow cytometer; Becton Dickinson, Heidelberg, Germany) using the cellquest program (Becton Dickinson).

    Article Title: TGF-beta-RII binding proteins
    Article Snippet: .. Anti-TGF-βRII IgG antibodies were captured on a CM5 sensor chip surface by an immobilized anti-CH1 antibody (Ab0669; BD cat. no. BD555784), followed by the addition of human recombinant TGF-βRII (R&D systems cat. no. 241-R2/CF; region Ile24-Aps195). ..

    Article Title: Inhibition of phosphoantigen-mediated ?? T-cell proliferation by CD4 + CD25 + FoxP3 + regulatory T cells
    Article Snippet: .. Reagents The following compounds, monoclonal antibodies (mAbs), assays and kits were used in this study: BrHPP (kindly provided by Innate Pharma, Marseilles, France), recombinant human IL-2 (Proleukin; Novartis, Basel, Switzerland), the anti-human FoxP3 Staining Kit (eBiosciences, San Diego, CA), interferon (IFN)-γ secretion assay, CD4 + CD25 + Treg isolation kit and anti-PE microbeads (all from Miltenyi Biotec, Bergisch Gladbach, Germany), recombinant human (rh) cytomegalovirus (CMV) pp65 protein (Miltenyi Biotec) 1-methyl- l -tryptophan, phytohaemagglutinin (PHA), the PKH26 red fluorescent cell linker mini kit (Sigma-Aldrich, Taufkirchen, Germany), anti-TGF-β mAb, recombinant human TGF-β soluble receptor II (sRII), anti-IL-10 mAb, human latency-associated petide (LAP; R&D Systems, Wiesbaden, Germany), proteinase K (Merck, Darmstadt, Germany), ToPro-3 iodide, the Vybrant®CFDA SE cell tracer kit (Molecular Probes, Eugene, OR) granulocyte–macrophage colony-stimulating factor (GM-CSF), IL-4, IL-15, IL-1β, IL-6, TNF-α (Tebu-Bio, Offenbach, Germany), prostaglandin E2 (Biomol, Hamburg, Germany), iTAg Tetramer/PE-HLA-A*0201 CMV pp65 (NLVPMVATV; Beckmann Coulter Immunomics Operations, Villepinte Roissy, France), N G -monomethyl- l -arginine ( l -NMMA) and N ω -hydroxy-nor- l -arginine (nor-NOHA; Axxora, Grünberg, Germany). .. Flow cytometric analysis Cells were harvested after the indicated * culture periods and analysed by two- or three-colour flow cytometry (FACScan flow cytometer; Becton Dickinson, Heidelberg, Germany) using the cellquest program (Becton Dickinson).

    Article Title: Immunomodulation of human CD19 + CD25 high regulatory B cells via Th17/Foxp3 regulatory T cells and Th1/Th2 cytokines.
    Article Snippet: Regulatory B (Breg) cells are a special subset of immunoregulatory cells with unique phenotypes and functions.. In this study, human CD19CD25 Breg cells were purified from human peripheral blood.. Based on the coculture system of Breg cells and CD4 T cells in vitro, Breg cells were found to promote the increase in regulatory T (Treg) cells while decreasing the number of Th17 cells.

    Bioprocessing:

    Article Title: Inhibition of phosphoantigen-mediated ?? T-cell proliferation by CD4 + CD25 + FoxP3 + regulatory T cells
    Article Snippet: .. The following compounds, monoclonal antibodies (mAbs), assays and kits were used in this study: BrHPP (kindly provided by Innate Pharma, Marseilles, France), recombinant human IL-2 (Proleukin; Novartis, Basel, Switzerland), the anti-human FoxP3 Staining Kit (eBiosciences, San Diego, CA), interferon (IFN)-γ secretion assay, CD4 + CD25 + Treg isolation kit and anti-PE microbeads (all from Miltenyi Biotec, Bergisch Gladbach, Germany), recombinant human (rh) cytomegalovirus (CMV) pp65 protein (Miltenyi Biotec) 1-methyl- l -tryptophan, phytohaemagglutinin (PHA), the PKH26 red fluorescent cell linker mini kit (Sigma-Aldrich, Taufkirchen, Germany), anti-TGF-β mAb, recombinant human TGF-β soluble receptor II (sRII), anti-IL-10 mAb, human latency-associated petide (LAP; R&D Systems, Wiesbaden, Germany), proteinase K (Merck, Darmstadt, Germany), ToPro-3 iodide, the Vybrant®CFDA SE cell tracer kit (Molecular Probes, Eugene, OR) granulocyte–macrophage colony-stimulating factor (GM-CSF), IL-4, IL-15, IL-1β, IL-6, TNF-α (Tebu-Bio, Offenbach, Germany), prostaglandin E2 (Biomol, Hamburg, Germany), iTAg Tetramer/PE-HLA-A*0201 CMV pp65 (NLVPMVATV; Beckmann Coulter Immunomics Operations, Villepinte Roissy, France), N G -monomethyl- l -arginine ( l -NMMA) and N ω -hydroxy-nor- l -arginine (nor-NOHA; Axxora, Grünberg, Germany). .. Cells were harvested after the indicated * culture periods and analysed by two- or three-colour flow cytometry (FACScan flow cytometer; Becton Dickinson, Heidelberg, Germany) using the cellquest program (Becton Dickinson).

    Article Title: Inhibition of phosphoantigen-mediated ?? T-cell proliferation by CD4 + CD25 + FoxP3 + regulatory T cells
    Article Snippet: .. Reagents The following compounds, monoclonal antibodies (mAbs), assays and kits were used in this study: BrHPP (kindly provided by Innate Pharma, Marseilles, France), recombinant human IL-2 (Proleukin; Novartis, Basel, Switzerland), the anti-human FoxP3 Staining Kit (eBiosciences, San Diego, CA), interferon (IFN)-γ secretion assay, CD4 + CD25 + Treg isolation kit and anti-PE microbeads (all from Miltenyi Biotec, Bergisch Gladbach, Germany), recombinant human (rh) cytomegalovirus (CMV) pp65 protein (Miltenyi Biotec) 1-methyl- l -tryptophan, phytohaemagglutinin (PHA), the PKH26 red fluorescent cell linker mini kit (Sigma-Aldrich, Taufkirchen, Germany), anti-TGF-β mAb, recombinant human TGF-β soluble receptor II (sRII), anti-IL-10 mAb, human latency-associated petide (LAP; R&D Systems, Wiesbaden, Germany), proteinase K (Merck, Darmstadt, Germany), ToPro-3 iodide, the Vybrant®CFDA SE cell tracer kit (Molecular Probes, Eugene, OR) granulocyte–macrophage colony-stimulating factor (GM-CSF), IL-4, IL-15, IL-1β, IL-6, TNF-α (Tebu-Bio, Offenbach, Germany), prostaglandin E2 (Biomol, Hamburg, Germany), iTAg Tetramer/PE-HLA-A*0201 CMV pp65 (NLVPMVATV; Beckmann Coulter Immunomics Operations, Villepinte Roissy, France), N G -monomethyl- l -arginine ( l -NMMA) and N ω -hydroxy-nor- l -arginine (nor-NOHA; Axxora, Grünberg, Germany). .. Flow cytometric analysis Cells were harvested after the indicated * culture periods and analysed by two- or three-colour flow cytometry (FACScan flow cytometer; Becton Dickinson, Heidelberg, Germany) using the cellquest program (Becton Dickinson).

    Staining:

    Article Title: Inhibition of phosphoantigen-mediated ?? T-cell proliferation by CD4 + CD25 + FoxP3 + regulatory T cells
    Article Snippet: .. The following compounds, monoclonal antibodies (mAbs), assays and kits were used in this study: BrHPP (kindly provided by Innate Pharma, Marseilles, France), recombinant human IL-2 (Proleukin; Novartis, Basel, Switzerland), the anti-human FoxP3 Staining Kit (eBiosciences, San Diego, CA), interferon (IFN)-γ secretion assay, CD4 + CD25 + Treg isolation kit and anti-PE microbeads (all from Miltenyi Biotec, Bergisch Gladbach, Germany), recombinant human (rh) cytomegalovirus (CMV) pp65 protein (Miltenyi Biotec) 1-methyl- l -tryptophan, phytohaemagglutinin (PHA), the PKH26 red fluorescent cell linker mini kit (Sigma-Aldrich, Taufkirchen, Germany), anti-TGF-β mAb, recombinant human TGF-β soluble receptor II (sRII), anti-IL-10 mAb, human latency-associated petide (LAP; R&D Systems, Wiesbaden, Germany), proteinase K (Merck, Darmstadt, Germany), ToPro-3 iodide, the Vybrant®CFDA SE cell tracer kit (Molecular Probes, Eugene, OR) granulocyte–macrophage colony-stimulating factor (GM-CSF), IL-4, IL-15, IL-1β, IL-6, TNF-α (Tebu-Bio, Offenbach, Germany), prostaglandin E2 (Biomol, Hamburg, Germany), iTAg Tetramer/PE-HLA-A*0201 CMV pp65 (NLVPMVATV; Beckmann Coulter Immunomics Operations, Villepinte Roissy, France), N G -monomethyl- l -arginine ( l -NMMA) and N ω -hydroxy-nor- l -arginine (nor-NOHA; Axxora, Grünberg, Germany). .. Cells were harvested after the indicated * culture periods and analysed by two- or three-colour flow cytometry (FACScan flow cytometer; Becton Dickinson, Heidelberg, Germany) using the cellquest program (Becton Dickinson).

    Article Title: Inhibition of phosphoantigen-mediated ?? T-cell proliferation by CD4 + CD25 + FoxP3 + regulatory T cells
    Article Snippet: .. Reagents The following compounds, monoclonal antibodies (mAbs), assays and kits were used in this study: BrHPP (kindly provided by Innate Pharma, Marseilles, France), recombinant human IL-2 (Proleukin; Novartis, Basel, Switzerland), the anti-human FoxP3 Staining Kit (eBiosciences, San Diego, CA), interferon (IFN)-γ secretion assay, CD4 + CD25 + Treg isolation kit and anti-PE microbeads (all from Miltenyi Biotec, Bergisch Gladbach, Germany), recombinant human (rh) cytomegalovirus (CMV) pp65 protein (Miltenyi Biotec) 1-methyl- l -tryptophan, phytohaemagglutinin (PHA), the PKH26 red fluorescent cell linker mini kit (Sigma-Aldrich, Taufkirchen, Germany), anti-TGF-β mAb, recombinant human TGF-β soluble receptor II (sRII), anti-IL-10 mAb, human latency-associated petide (LAP; R&D Systems, Wiesbaden, Germany), proteinase K (Merck, Darmstadt, Germany), ToPro-3 iodide, the Vybrant®CFDA SE cell tracer kit (Molecular Probes, Eugene, OR) granulocyte–macrophage colony-stimulating factor (GM-CSF), IL-4, IL-15, IL-1β, IL-6, TNF-α (Tebu-Bio, Offenbach, Germany), prostaglandin E2 (Biomol, Hamburg, Germany), iTAg Tetramer/PE-HLA-A*0201 CMV pp65 (NLVPMVATV; Beckmann Coulter Immunomics Operations, Villepinte Roissy, France), N G -monomethyl- l -arginine ( l -NMMA) and N ω -hydroxy-nor- l -arginine (nor-NOHA; Axxora, Grünberg, Germany). .. Flow cytometric analysis Cells were harvested after the indicated * culture periods and analysed by two- or three-colour flow cytometry (FACScan flow cytometer; Becton Dickinson, Heidelberg, Germany) using the cellquest program (Becton Dickinson).

    Isolation:

    Article Title: Inhibition of phosphoantigen-mediated ?? T-cell proliferation by CD4 + CD25 + FoxP3 + regulatory T cells
    Article Snippet: .. The following compounds, monoclonal antibodies (mAbs), assays and kits were used in this study: BrHPP (kindly provided by Innate Pharma, Marseilles, France), recombinant human IL-2 (Proleukin; Novartis, Basel, Switzerland), the anti-human FoxP3 Staining Kit (eBiosciences, San Diego, CA), interferon (IFN)-γ secretion assay, CD4 + CD25 + Treg isolation kit and anti-PE microbeads (all from Miltenyi Biotec, Bergisch Gladbach, Germany), recombinant human (rh) cytomegalovirus (CMV) pp65 protein (Miltenyi Biotec) 1-methyl- l -tryptophan, phytohaemagglutinin (PHA), the PKH26 red fluorescent cell linker mini kit (Sigma-Aldrich, Taufkirchen, Germany), anti-TGF-β mAb, recombinant human TGF-β soluble receptor II (sRII), anti-IL-10 mAb, human latency-associated petide (LAP; R&D Systems, Wiesbaden, Germany), proteinase K (Merck, Darmstadt, Germany), ToPro-3 iodide, the Vybrant®CFDA SE cell tracer kit (Molecular Probes, Eugene, OR) granulocyte–macrophage colony-stimulating factor (GM-CSF), IL-4, IL-15, IL-1β, IL-6, TNF-α (Tebu-Bio, Offenbach, Germany), prostaglandin E2 (Biomol, Hamburg, Germany), iTAg Tetramer/PE-HLA-A*0201 CMV pp65 (NLVPMVATV; Beckmann Coulter Immunomics Operations, Villepinte Roissy, France), N G -monomethyl- l -arginine ( l -NMMA) and N ω -hydroxy-nor- l -arginine (nor-NOHA; Axxora, Grünberg, Germany). .. Cells were harvested after the indicated * culture periods and analysed by two- or three-colour flow cytometry (FACScan flow cytometer; Becton Dickinson, Heidelberg, Germany) using the cellquest program (Becton Dickinson).

    Article Title: Inhibition of phosphoantigen-mediated ?? T-cell proliferation by CD4 + CD25 + FoxP3 + regulatory T cells
    Article Snippet: .. Reagents The following compounds, monoclonal antibodies (mAbs), assays and kits were used in this study: BrHPP (kindly provided by Innate Pharma, Marseilles, France), recombinant human IL-2 (Proleukin; Novartis, Basel, Switzerland), the anti-human FoxP3 Staining Kit (eBiosciences, San Diego, CA), interferon (IFN)-γ secretion assay, CD4 + CD25 + Treg isolation kit and anti-PE microbeads (all from Miltenyi Biotec, Bergisch Gladbach, Germany), recombinant human (rh) cytomegalovirus (CMV) pp65 protein (Miltenyi Biotec) 1-methyl- l -tryptophan, phytohaemagglutinin (PHA), the PKH26 red fluorescent cell linker mini kit (Sigma-Aldrich, Taufkirchen, Germany), anti-TGF-β mAb, recombinant human TGF-β soluble receptor II (sRII), anti-IL-10 mAb, human latency-associated petide (LAP; R&D Systems, Wiesbaden, Germany), proteinase K (Merck, Darmstadt, Germany), ToPro-3 iodide, the Vybrant®CFDA SE cell tracer kit (Molecular Probes, Eugene, OR) granulocyte–macrophage colony-stimulating factor (GM-CSF), IL-4, IL-15, IL-1β, IL-6, TNF-α (Tebu-Bio, Offenbach, Germany), prostaglandin E2 (Biomol, Hamburg, Germany), iTAg Tetramer/PE-HLA-A*0201 CMV pp65 (NLVPMVATV; Beckmann Coulter Immunomics Operations, Villepinte Roissy, France), N G -monomethyl- l -arginine ( l -NMMA) and N ω -hydroxy-nor- l -arginine (nor-NOHA; Axxora, Grünberg, Germany). .. Flow cytometric analysis Cells were harvested after the indicated * culture periods and analysed by two- or three-colour flow cytometry (FACScan flow cytometer; Becton Dickinson, Heidelberg, Germany) using the cellquest program (Becton Dickinson).

    Cell Culture:

    Article Title: Immunomodulation of human CD19 + CD25 high regulatory B cells via Th17/Foxp3 regulatory T cells and Th1/Th2 cytokines.
    Article Snippet: Regulatory B (Breg) cells are a special subset of immunoregulatory cells with unique phenotypes and functions.. In this study, human CD19CD25 Breg cells were purified from human peripheral blood.. Based on the coculture system of Breg cells and CD4 T cells in vitro, Breg cells were found to promote the increase in regulatory T (Treg) cells while decreasing the number of Th17 cells.

    Concentration Assay:

    Article Title: Immunomodulation of human CD19 + CD25 high regulatory B cells via Th17/Foxp3 regulatory T cells and Th1/Th2 cytokines.
    Article Snippet: Regulatory B (Breg) cells are a special subset of immunoregulatory cells with unique phenotypes and functions.. In this study, human CD19CD25 Breg cells were purified from human peripheral blood.. Based on the coculture system of Breg cells and CD4 T cells in vitro, Breg cells were found to promote the increase in regulatory T (Treg) cells while decreasing the number of Th17 cells.



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