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Molecular Research Center inc 1x hybridization buffer
1x Hybridization Buffer, supplied by Molecular Research Center inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1x+zymogram+developing+buffer/hybridization+buffer+hs114/pmc04547522-97-4-7
Average 90 stars, based on 1 article reviews
1x hybridization buffer - by Bioz Stars, 2026-10
90/100 stars

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Hybridization:

Article Title: Coupling Molecular Beacons to Barcoded Metal Nanowires for Multiplexed, Sealed Chamber DNA Bioassays
Article Snippet: .. Buffers used in the experiments were: (1) 0.3 M PBS (0.3 M NaCl and 10 mM sodium phosphate, pH 7.0), (2) 40 mM citrate (40 mM citrate in 0.3 M PBS), (3) 0.01 M PBS buffer (0.138 M NaCl; 0.0027 M KCl; 10 mM sodium phosphate, pH 7.4), purchased from Sigma, (4) commercial hybridization buffer (HS114), obtained from Molecular Research Centers, Inc., (5) Tris (100 mM MgCl 2 , 20 mM Tris-HCl, pH 8.0), and (6) CAC buffer (0.5 M NaCl, 20 mM cacodylic acid, and 0.5 mM EDTA, pH 7.0). ..

Article Title: Coupling molecular beacons to barcoded metal nanowires for multiplexed, sealed chamber DNA bioassays.
Article Snippet: .. Buffers used in the experiments were: (1) 0.3 M PBS (0.3 M NaCl and 10 mM sodium phosphate, pH 7.0), (2) 40 mM citrate (40 mM citrate in 0.3 M PBS), (3) 0.01 M PBS buffer (0.138 M NaCl; 0.0027 M KCl; 10 mM sodium phosphate, pH 7.4), purchased from Sigma, (4) commercial hybridization buffer (HS114), obtained from Molecular Research Centers, Inc., (5) Tris (100 mM MgCl2, 20 mM Tris-HCl, pH 8.0), and (6) CAC buffer (0.5 M NaCl, 20 mM cacodylic acid, and 0.5 mM EDTA, pH 7.0). ..

Article Title: Distance-Dependent Emission from Dye-Labeled Oligonucleotides on Striped Au/Ag Nanowires: Effect of Secondary Structure and Hybridization Efficiency
Article Snippet: Hybridization buffer (0.3 M PBS) was made in-house and was prepared using 0.3 M NaCl and 10 mM sodium phosphate at pH 7.0, and 0.01 M PBS buffer (0.01 M phosphate buffered saline; 0.138 M NaCl; 0.0027 M KCl; pH 7.4) was purchased from Sigma. .. Commercial hybridization buffer (HS114) was obtained from Molecular Research Centers, Inc. CAC buffer was prepared according to Krauss and co-workers, using 0.5 M NaCl, 20 mM cacodylic acid, and 0.5 mM EDTA, pH = 7. ..

Article Title: Phytoestrogens Regulate mRNA and Protein Levels of Guanine Nucleotide-Binding Protein, Beta-1 Subunit (GNB1) in MCF-7 Cells
Article Snippet: Membranes were hybridized with 1.0–3.0 × 10 6 cpm/mL of probe overnight at 50°C in a Hybaid rotisserie hybridization oven (Labnet) using ExpressHyb hybridization solution (Clontech). .. Membranes were washed with 1X hybridization buffer (Molecular Research Center Inc., Cincinnati, OH, USA) at 50°C for 1 h, followed by 2X SSC/0.5% SDS at 50°C for 30 min. To verify equal loading of RNA, the membrane was stripped (boiling solution of 0.5% SDS in 40 mM Tris-HCl pH 7.5, 10% glycerol and 2.0 mM EDTA) and re-hybridized with a 350 bp fragment of the GAPDH gene as the probe. ..

Article Title: Characterization of Bizzy Nut extracts in estrogen-responsive MCF-7 breast cancer cells.
Article Snippet: Kola acuminate, also known as Bizzy Nut or Kola Nut, is a natural product that contains bioactive chemicals that possess hormonal properties.. The purpose of this study was to characterize the putative phytoestrogenic compounds present in Bizzy Nut for estrogenic-like activity.. As an initial step, five extracts (E1 – hexane, E2 – ether, E3 – acetone, E4 – methanol and E5 – water) were sequentially generated using solid–liquid phase extraction and their bioactivity was examined in MCF-7, MDA-MB-468 and LNCaP cancer cell models.

Article Title: Phytoestrogens Regulate mRNA and Protein Levels of Guanine Nucleotide-Binding Protein, Beta-1 Subunit (GNB1) in MCF-7 Cells
Article Snippet: The membrane arrays were hybridized with 2 × 10 6 cpm/mL of probe overnight at 50°C in a Hybaid rotisserie hybridization oven (Labnet, Woodbridge, NJ, USA) using ExpressHyb hybridization solution (Clontech). .. Membranes were washed with 1X hybridization buffer (Molecular Research Center Inc., Cincinnati, OH) at 50°C for 1 h, followed by 2X SSC/0.5% SDS at 50°C for 30 min. .. Preparation of cDNA Probe Both 32 P-labeled cDNA probes (GE-induced cDNA probe, 17-β E 2- induced cDNA probe) were prepared according to the procedures of Ramanathan et al. ( Ramanathan and Gray, 2003 ).

other:

Article Title: Impairment of ribosomal subunit synthesis in aminoglycoside treated ribonuclease mutants of Escherichia coli
Article Snippet: The membranes were pre-hybridized in 15mL of 1X pre-hybridization solution (MRC, Inc.) at 42°C for 30 minutes.

Membrane:

Article Title: Phytoestrogens Regulate mRNA and Protein Levels of Guanine Nucleotide-Binding Protein, Beta-1 Subunit (GNB1) in MCF-7 Cells
Article Snippet: Membranes were hybridized with 1.0–3.0 × 10 6 cpm/mL of probe overnight at 50°C in a Hybaid rotisserie hybridization oven (Labnet) using ExpressHyb hybridization solution (Clontech). .. Membranes were washed with 1X hybridization buffer (Molecular Research Center Inc., Cincinnati, OH, USA) at 50°C for 1 h, followed by 2X SSC/0.5% SDS at 50°C for 30 min. To verify equal loading of RNA, the membrane was stripped (boiling solution of 0.5% SDS in 40 mM Tris-HCl pH 7.5, 10% glycerol and 2.0 mM EDTA) and re-hybridized with a 350 bp fragment of the GAPDH gene as the probe. ..

Article Title: Characterization of Bizzy Nut extracts in estrogen-responsive MCF-7 breast cancer cells.
Article Snippet: Kola acuminate, also known as Bizzy Nut or Kola Nut, is a natural product that contains bioactive chemicals that possess hormonal properties.. The purpose of this study was to characterize the putative phytoestrogenic compounds present in Bizzy Nut for estrogenic-like activity.. As an initial step, five extracts (E1 – hexane, E2 – ether, E3 – acetone, E4 – methanol and E5 – water) were sequentially generated using solid–liquid phase extraction and their bioactivity was examined in MCF-7, MDA-MB-468 and LNCaP cancer cell models.



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