anti gal4 ad sc 1663 antibodies (Santa Cruz Biotechnology)
Santa Cruz Biotechnology is a verified supplier 
95
Structured Review
Santa Cruz Biotechnology
anti gal4 ad sc 1663 antibodies
![SUP physically interacts with TPL through its EAR motif (DLELRL). ( A ) Yeast two-hybrid assays testing interaction between TPL [fused to the <t>GAL4</t> DNA-binding domain (DBD)] and wild-type SUP (DLELRL), mSUP (DLE A R A ) or mSUP (DLE G R G ) [each fused to the <t>GAL4</t> <t>activation</t> <t>domain</t> (AD)]. Growth on selection (right panel) detects a physical interaction between TPL and wild-type SUP (DLELRL) only. ( B ) In vitro pull-down assays using GST-tagged N-TPL show a strong interaction with SBP-tagged wild-type SUP (DLELRL) that is weakened by mutation of the EAR motif in SUP (DLE A R A ). Ponceau S staining shows efficient GST protein expression. Full-length blots and quantification of Western blot signal intensities are presented in Supplementary Fig. S3](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5690/pmc12595690/pmc12595690__12870_2025_7553_Fig2_HTML.jpg)
Anti Gal4 Ad Sc 1663 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 744 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1663/GAL4/pmc12595690-66-28-32
Average 95 stars, based on 744 article reviews
Anti Gal4 Ad Sc 1663 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 744 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1663/GAL4/pmc12595690-66-28-32
Average 95 stars, based on 744 article reviews
anti gal4 ad sc 1663 antibodies - by Bioz Stars,
2026-11
95/100 stars
Images
1) Product Images from "SUPERMAN requires its EAR motif to recruit the corepressor TOPLESS and to function as a cadastral regulator in floral patterning"
Article Title: SUPERMAN requires its EAR motif to recruit the corepressor TOPLESS and to function as a cadastral regulator in floral patterning
Journal: BMC Plant Biology
doi: 10.1186/s12870-025-07553-z
Figure Legend Snippet: SUP physically interacts with TPL through its EAR motif (DLELRL). ( A ) Yeast two-hybrid assays testing interaction between TPL [fused to the GAL4 DNA-binding domain (DBD)] and wild-type SUP (DLELRL), mSUP (DLE A R A ) or mSUP (DLE G R G ) [each fused to the GAL4 activation domain (AD)]. Growth on selection (right panel) detects a physical interaction between TPL and wild-type SUP (DLELRL) only. ( B ) In vitro pull-down assays using GST-tagged N-TPL show a strong interaction with SBP-tagged wild-type SUP (DLELRL) that is weakened by mutation of the EAR motif in SUP (DLE A R A ). Ponceau S staining shows efficient GST protein expression. Full-length blots and quantification of Western blot signal intensities are presented in Supplementary Fig. S3
Techniques Used: Binding Assay, Activation Assay, Selection, In Vitro, Mutagenesis, Staining, Expressing, Western Blot
![CRISPR-generated EAR mutations disrupt physical interaction between SUP and TPL. ( A ) Yeast two-hybrid assays testing interaction between TPL [fused to the GAL4 DNA-binding domain (DBD)] and wild-type SUP (DLELRL), mSUP (DLELL) or mSUP (∆EAR) [each fused to the GAL4 activation domain (AD)]. Growth on selection (right panel) indicates physical interaction between TPL and wild-type SUP (DLELRL) only. ( B ) In vitro pull-down assays using GST-tagged N-TPL show a strong interaction with SBP-tagged wild-type SUP (DLELRL) which is weakened by mutation of the EAR motif in CRISPR allele mSUP (DLELL). Ponceau S staining shows efficient GST protein expression. Full-length blots and quantification of Western blot signal intensities are presented in Supplementary Fig. S7](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5690/pmc12595690/pmc12595690__12870_2025_7553_Fig5_HTML.jpg "... assays testing interaction between TPL [fused to the GAL4 DNA-binding domain (DBD)] and wild-type SUP (DLELRL), mSUP ...")
Figure Legend Snippet: CRISPR-generated EAR mutations disrupt physical interaction between SUP and TPL. ( A ) Yeast two-hybrid assays testing interaction between TPL [fused to the GAL4 DNA-binding domain (DBD)] and wild-type SUP (DLELRL), mSUP (DLELL) or mSUP (∆EAR) [each fused to the GAL4 activation domain (AD)]. Growth on selection (right panel) indicates physical interaction between TPL and wild-type SUP (DLELRL) only. ( B ) In vitro pull-down assays using GST-tagged N-TPL show a strong interaction with SBP-tagged wild-type SUP (DLELRL) which is weakened by mutation of the EAR motif in CRISPR allele mSUP (DLELL). Ponceau S staining shows efficient GST protein expression. Full-length blots and quantification of Western blot signal intensities are presented in Supplementary Fig. S7
Techniques Used: CRISPR, Generated, Binding Assay, Activation Assay, Selection, In Vitro, Mutagenesis, Staining, Expressing, Western Blot
Related Articles
Imaging:Article Title: Identification and validation of an interaction between the E3 Ubiquitin Ligase WWP1 and the transcriptional co-activator WBP2 in the human heart. Article Snippet: Membranes were then incubated at 4 ̊C overnight in primary antibodies (mouse anti-yeast GAL 4 DNA-binding domain, clone RK5C1, Santa Cruz Biotechnology #sc510, RRID:AB_627655, 1:200; mouse anti-human WWP1, Abnova #H00011059-M01, RRID:AB_509107, 1:500; rabbit anti-yeast -tubulin, clone EPR13799, Abcam #ab184970, RRID:AB_2928998,1:15,000) diluted in blocking buffer. .. Species specific HRP-conjugated secondary antibodies (sheep anti-mouse IgG #NA931, GE Healthcare or goat anti-rabbit IgG #7074S, Cell Signaling Technologies) and SuperSignal West Femto Maximum Signaling Substrate (ThermoFisher) were used for signal detection and Expressing:Article Title: A direct interaction between the Chd1 CHCT domain and Rtf1 controls Chd1 distribution and nucleosome positioning on active genes Article Snippet: Library quality was verified using fragment analysis (Fragment Analyzer, Advanced Analytical) and submitted for sequencing on an Illumina NextSeq 500 or 2000 instrument for paired-end sequencing to a depth of ∼15 million uniquely mapped reads per sample. .. Experiments analyzing the expression of yeast Rtf1 fusions to the Article Title: SUPERMAN requires its EAR motif to recruit the corepressor TOPLESS and to function as a cadastral regulator in floral patterning. Article Snippet: .. To verify yeast protein expression, Western blots were stained with 0.1% Ponceau S in 5% v/v acetic acid, washed in water, and analyzed using Article Title: SUPERMAN requires its EAR motif to recruit the corepressor TOPLESS and to function as a cadastral regulator in floral patterning Article Snippet: .. To verify yeast protein expression, Western blots were stained with 0.1% Ponceau S in 5% v/v acetic acid, washed in water, and analyzed using Western Blot:Article Title: A direct interaction between the Chd1 CHCT domain and Rtf1 controls Chd1 distribution and nucleosome positioning on active genes Article Snippet: Library quality was verified using fragment analysis (Fragment Analyzer, Advanced Analytical) and submitted for sequencing on an Illumina NextSeq 500 or 2000 instrument for paired-end sequencing to a depth of ∼15 million uniquely mapped reads per sample. .. Experiments analyzing the expression of yeast Rtf1 fusions to the Article Title: SUPERMAN requires its EAR motif to recruit the corepressor TOPLESS and to function as a cadastral regulator in floral patterning. Article Snippet: .. To verify yeast protein expression, Western blots were stained with 0.1% Ponceau S in 5% v/v acetic acid, washed in water, and analyzed using Article Title: SUPERMAN requires its EAR motif to recruit the corepressor TOPLESS and to function as a cadastral regulator in floral patterning Article Snippet: .. To verify yeast protein expression, Western blots were stained with 0.1% Ponceau S in 5% v/v acetic acid, washed in water, and analyzed using Staining:Article Title: SUPERMAN requires its EAR motif to recruit the corepressor TOPLESS and to function as a cadastral regulator in floral patterning. Article Snippet: .. To verify yeast protein expression, Western blots were stained with 0.1% Ponceau S in 5% v/v acetic acid, washed in water, and analyzed using Article Title: SUPERMAN requires its EAR motif to recruit the corepressor TOPLESS and to function as a cadastral regulator in floral patterning Article Snippet: .. To verify yeast protein expression, Western blots were stained with 0.1% Ponceau S in 5% v/v acetic acid, washed in water, and analyzed using Incubation:Article Title: Development of a TEAD-independent reporter system for specific and sensitive measurement of YAP/TAZ activity. Article Snippet: Accurate quantification of Yes-associated protein (YAP) and its paralog TAZ is critical for understanding their biological functions, however, existing reporter systems are constrained by their reliance on endogenous TEAD transcription factors.. Here, we developed an endogenous TEAD-independent reporter system for YAP/TAZ activity, based on the GAL4-UAS system.. The construct consists of the GAL4 DNA-binding domain (DBD) fused to the YAP-binding domain (YBD) of TEAD4, with the native TEAD DNA-binding domain (TEA) removed. other:Article Title: NUDIX hydrolases target specific inositol pyrophosphates and regulate phosphate homeostasis and bacterial pathogen susceptibility in Arabidopsis. Article Snippet: After 2 weeks, the seedlings were transferred to soil and plant gDNA was extracted from leaves using the Phire Plant Direct PCR Kit (Thermo Fisher) following the manufacturer's instructions. Control:Article Title: NUDIX hydrolases target specific inositol pyrophosphates and regulate phosphate homeostasis and bacterial pathogen susceptibility in Arabidopsis Article Snippet: V5‐tagged proteins were detected using a mouse anti‐V5 primary antibody (R960‐25; 1:2,000 dilution; Invitrogen), followed by an Alexa Fluor Plus 800‐conjugated goat anti‐mouse antibody (1:20,000; Invitrogen). .. |
