snat2 (Santa Cruz Biotechnology)
Santa Cruz Biotechnology is a verified supplier 
93
Structured Review
Santa Cruz Biotechnology
snat2
Snat2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1663/SNAT2+Antibody/pmc06526116-44-89-92
Average 93 stars, based on 60 article reviews
Snat2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 60 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1663/SNAT2+Antibody/pmc06526116-44-89-92
Average 93 stars, based on 60 article reviews
snat2 - by Bioz Stars,
2026-10
93/100 stars
Images
1) Product Images from "Glutamine Supplementation Ameliorates Chronic Stress-induced Reductions in Glutamate and Glutamine Transporters in the Mouse Prefrontal Cortex"
Article Title: Glutamine Supplementation Ameliorates Chronic Stress-induced Reductions in Glutamate and Glutamine Transporters in the Mouse Prefrontal Cortex
Journal: Experimental Neurobiology
doi: 10.5607/en.2019.28.2.270
Figure Legend Snippet: The glutamate/glutamine cycle. High-affinity excitatory amino acid transporters (EAATs) of astrocytes primarily clear Glu in the synaptic cleft that is released from presynaptic neurons. GLT1 (glutamate transporter 1/EAAT2/SLC1A2) is the major regulator that transports Glu to astrocytes. In astrocytes, Glu is converted into Gln by glutamine synthetase (GS). Synthesized Gln is released from the astrocytes through sodium-coupled neutral amino acid transporters (SNAT3/SLC38A3 and SNAT5/SLC38A5). Released Gln is transported into neurons via SNAT1 and SNAT2 (SLC38A1 and SLC38A2) and converted back to Glu by phosphate-activated glutaminase (PAG or GLS1/2, mainly GLS2 in brain) for synaptic transmission of glutamatergic neurons. Glutamate is further transformed to γ-aminobutyric acid (GABA) by glutamic acid decarboxylases (GAD1 and GAD2) in the GABAergic neurons. Glu can be synthesized in the astrocytes from glucose via the tricarboxylic acid (TCA) cycle, followed by transamination of α-ketoglutarate (α-KG). Pyruvate carboxylase (PC) converts pyruvate to oxaloacetate that is converted to α-KG via the TCA cycle. Glutamate dehydrogenase (GDH) connects the TCA and Glu-Gln cycles by reversibly converting Glu into α-KG and vice versa. Expressional changes by chronic immobilization stress (CIS) and Gln supplementation are marked by red and green arrows, respectively.
Techniques Used: Synthesized, Transmission Assay, Transformation Assay
Related Articles
Immunostaining:Article Title: ZNF469 couples amino acid transport and precursor biosynthesis to collagen production in fibrosis Article Snippet: Images were acquired using an EVOS M7000 Imaging System (Thermo Fisher Scientific) for three random fields per independent experiment, and measurements were quantified utilizing ImageJ software (version 1.54 g) and averaged for representation. .. To evaluate protein-level changes in amino acid transporters and biosynthetic enzymes upon ZNF469 depletion, additional immunostaining was performed using primary antibodies against SLC1A5 (cat. no. A23156; ABclonal, Woburn, MA, USA), SLC3A2 (cat. no. A24735; ABclonal), SLC7A11 (cat. no. A25302; ABclonal), SLC38A1 (cat. no. N104/37; Developmental Studies Hybridoma Bank), Blocking Assay:Article Title: Metabolic engineering of SLC38A2 reprograms glutamine utilization and enhances CAR-macrophage antitumor function in solid tumors. Article Snippet: .. After blocking, cells were incubated overnight at 4°C with CD68 (E3O7V) rabbit monoclonal antibody (1: 500, cat# 97778, CST) and Incubation:Article Title: Metabolic engineering of SLC38A2 reprograms glutamine utilization and enhances CAR-macrophage antitumor function in solid tumors. Article Snippet: .. After blocking, cells were incubated overnight at 4°C with CD68 (E3O7V) rabbit monoclonal antibody (1: 500, cat# 97778, CST) and Article Title: Connexin43 Deficiency Leads to Ventricular Arrhythmias by Reprogramming Proline Metabolism Article Snippet: Calcium signaling was made by recording the fluorescence of cells using an Ultra High Speed Wavelength Switcher (Lambda DG‐4, Sutter Instruments) with a CCD camera (Zyla, Andor) mounted on an inverted microscope (Eclipse Ti, Nikon). .. The iPSC‐CMs and mouse ventricular tissues were first lysed and lysates then incubated with the primary antibodies (Cx43 (Abcam, ab11370, 1:1000) and Article Title: Metabolic engineering of SLC38A2 reprograms glutamine utilization and enhances CAR-macrophage antitumor function in solid tumors. Article Snippet: The human ovarian cancer cell line SKOV3 and the human breast cancer cell line SKBR3 were cultured in McCoy’s 5A medium (Gibco, cat# 16600082) supplemented with 10% FBS and 1% penicillin-streptomycin under standard culture conditions. .. For surface marker staining and flow cytometric analysis, cells were incubated with the following antibodies: Whitlow/218 linker recombinant rabbit mAb (Alexa Fluor® 647 conjugate; cat# S0B1796-50T, Starter), other:Article Title: Connexin43 Deficiency Leads to Ventricular Arrhythmias by Reprogramming Proline Metabolism. Article Snippet: Data were analyzed with FlowJo by measuring the mean fluorescence intensity of each sample. Western Blot:Article Title: Connexin43 Deficiency Leads to Ventricular Arrhythmias by Reprogramming Proline Metabolism Article Snippet: Protein concentrations were measured via a BCA kit (Pierce, 23227). .. Western blot was performed using standard protocol with the following antibodies: Cx43 (Abcam, ab11370, 1:1000), DRP1 (Proteintech, 12957‐1‐AP, 1:500), phospho‐DRP1 (Ser616) (Cell signaling, 3455S, 1:1000), PRODH (Proteintech, 22980‐1‐AP, 1:500), Article Title: Connexin43 Deficiency Leads to Ventricular Arrhythmias by Reprogramming Proline Metabolism. Article Snippet: Protein concentrations were measured via a BCA kit (Pierce, 23227). .. Western blot was performed using standard protocol with the following antibodies: Cx43 (Abcam, ab11370, 1:1000), DRP1 (Proteintech, 12957-1-AP, 1:500), phospho-DRP1 (Ser616) (Cell signaling, 3455S, 1:1000), PRODH (Proteintech, 22980-1-AP, 1:500), Marker:Article Title: Metabolic engineering of SLC38A2 reprograms glutamine utilization and enhances CAR-macrophage antitumor function in solid tumors. Article Snippet: The human ovarian cancer cell line SKOV3 and the human breast cancer cell line SKBR3 were cultured in McCoy’s 5A medium (Gibco, cat# 16600082) supplemented with 10% FBS and 1% penicillin-streptomycin under standard culture conditions. .. For surface marker staining and flow cytometric analysis, cells were incubated with the following antibodies: Whitlow/218 linker recombinant rabbit mAb (Alexa Fluor® 647 conjugate; cat# S0B1796-50T, Starter), Staining:Article Title: Metabolic engineering of SLC38A2 reprograms glutamine utilization and enhances CAR-macrophage antitumor function in solid tumors. Article Snippet: The human ovarian cancer cell line SKOV3 and the human breast cancer cell line SKBR3 were cultured in McCoy’s 5A medium (Gibco, cat# 16600082) supplemented with 10% FBS and 1% penicillin-streptomycin under standard culture conditions. .. For surface marker staining and flow cytometric analysis, cells were incubated with the following antibodies: Whitlow/218 linker recombinant rabbit mAb (Alexa Fluor® 647 conjugate; cat# S0B1796-50T, Starter), Recombinant:Article Title: Metabolic engineering of SLC38A2 reprograms glutamine utilization and enhances CAR-macrophage antitumor function in solid tumors. Article Snippet: The human ovarian cancer cell line SKOV3 and the human breast cancer cell line SKBR3 were cultured in McCoy’s 5A medium (Gibco, cat# 16600082) supplemented with 10% FBS and 1% penicillin-streptomycin under standard culture conditions. .. For surface marker staining and flow cytometric analysis, cells were incubated with the following antibodies: Whitlow/218 linker recombinant rabbit mAb (Alexa Fluor® 647 conjugate; cat# S0B1796-50T, Starter), |
![SUP physically interacts with TPL through its EAR motif (DLELRL). ( A ) Yeast two-hybrid assays testing interaction between TPL [fused to the <t>GAL4</t> DNA-binding domain (DBD)] and wild-type SUP (DLELRL), mSUP (DLE A R A ) or mSUP (DLE G R G ) [each fused to the <t>GAL4</t> <t>activation</t> <t>domain</t> (AD)]. Growth on selection (right panel) detects a physical interaction between TPL and wild-type SUP (DLELRL) only. ( B ) In vitro pull-down assays using GST-tagged N-TPL show a strong interaction with SBP-tagged wild-type SUP (DLELRL) that is weakened by mutation of the EAR motif in SUP (DLE A R A ). Ponceau S staining shows efficient GST protein expression. Full-length blots and quantification of Western blot signal intensities are presented in Supplementary Fig. S3](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5690/pmc12595690/pmc12595690__12870_2025_7553_Fig2_HTML.jpg)
![CRISPR-generated EAR mutations disrupt physical interaction between SUP and TPL. ( A ) Yeast two-hybrid assays testing interaction between TPL [fused to the GAL4 DNA-binding domain (DBD)] and wild-type SUP (DLELRL), mSUP (DLELL) or mSUP (∆EAR) [each fused to the GAL4 activation domain (AD)]. Growth on selection (right panel) indicates physical interaction between TPL and wild-type SUP (DLELRL) only. ( B ) In vitro pull-down assays using GST-tagged N-TPL show a strong interaction with SBP-tagged wild-type SUP (DLELRL) which is weakened by mutation of the EAR motif in CRISPR allele mSUP (DLELL). Ponceau S staining shows efficient GST protein expression. Full-length blots and quantification of Western blot signal intensities are presented in Supplementary Fig. S7](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5690/pmc12595690/pmc12595690__12870_2025_7553_Fig5_HTML.jpg)