anti glutamate cysteine ligase catalytic subunit (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
anti glutamate cysteine ligase catalytic subunit
Anti Glutamate Cysteine Ligase Catalytic Subunit, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1663/%CE%B3-GCSc+Antibody/pmc04782222-50-47-53
Average 94 stars, based on 74 article reviews
Anti Glutamate Cysteine Ligase Catalytic Subunit, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1663/%CE%B3-GCSc+Antibody/pmc04782222-50-47-53
Average 94 stars, based on 74 article reviews
anti glutamate cysteine ligase catalytic subunit - by Bioz Stars,
2026-09
94/100 stars
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The sections were stained with antibodies against GPX4 (Abcam, ab125066, 1:500), other:Article Title: Systematic Evaluation Defines the Limits of Ferroptosis in Cancer Therapy Article Snippet: TXNRD1 (SC-28321), Incubation:Article Title: Arachidonic acid protects against diabetes-induced atrial fibrillation Article Snippet: Proteins were transferred to PVDF membranes (FL00010, Millipore, Massachusetts, USA), which were then blocked with 5% non-fat milk for 1 h at room temperature to prevent nonspecific binding. .. Membranes were incubated overnight at 4 °C with primary antibodies against target proteins, followed by incubation with the appropriate HRP-conjugated secondary antibodies at room temperature for 1 h. Protein bands were visualized and quantified using Image Lab software (Bio-Rad Laboratories, USA). β-actin was used as the loading control. ( β-actin (1:2000), AC004, ABclonal, Wuhan, China; γ-GCS (1:500), Article Title: Arachidonic acid protects against diabetes-induced atrial fibrillation. Article Snippet: Proteins were transferred 434 to PVDF membranes (FL00010, Millipore, Massachusetts, USA), 435 which were then blocked with 5% non-fat milk for 1 h at room 436 temperature to prevent nonspecific binding. .. Membranes were 437 incubated overnight at 4 °C with primary antibodies against target 438 proteins, followed by incubation with the appropriate HRP- AR TIC LE IN PR ES S 22 / 67 439 conjugated secondary antibodies at room temperature for 1 h. 440 Protein bands were visualized and quantified using Image Lab 441 software (Bio-Rad Laboratories, USA). β-actin was used as the 442 loading control. ( β-actin (1:2000), AC004, ABclonal, Wuhan, China; 443 γ-GCS (1:500), Software:Article Title: Arachidonic acid protects against diabetes-induced atrial fibrillation Article Snippet: Proteins were transferred to PVDF membranes (FL00010, Millipore, Massachusetts, USA), which were then blocked with 5% non-fat milk for 1 h at room temperature to prevent nonspecific binding. .. Membranes were incubated overnight at 4 °C with primary antibodies against target proteins, followed by incubation with the appropriate HRP-conjugated secondary antibodies at room temperature for 1 h. Protein bands were visualized and quantified using Image Lab software (Bio-Rad Laboratories, USA). β-actin was used as the loading control. ( β-actin (1:2000), AC004, ABclonal, Wuhan, China; γ-GCS (1:500), Article Title: Arachidonic acid protects against diabetes-induced atrial fibrillation. Article Snippet: Proteins were transferred 434 to PVDF membranes (FL00010, Millipore, Massachusetts, USA), 435 which were then blocked with 5% non-fat milk for 1 h at room 436 temperature to prevent nonspecific binding. .. Membranes were 437 incubated overnight at 4 °C with primary antibodies against target 438 proteins, followed by incubation with the appropriate HRP- AR TIC LE IN PR ES S 22 / 67 439 conjugated secondary antibodies at room temperature for 1 h. 440 Protein bands were visualized and quantified using Image Lab 441 software (Bio-Rad Laboratories, USA). β-actin was used as the 442 loading control. ( β-actin (1:2000), AC004, ABclonal, Wuhan, China; 443 γ-GCS (1:500), Control:Article Title: Arachidonic acid protects against diabetes-induced atrial fibrillation Article Snippet: Proteins were transferred to PVDF membranes (FL00010, Millipore, Massachusetts, USA), which were then blocked with 5% non-fat milk for 1 h at room temperature to prevent nonspecific binding. .. 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![SUP physically interacts with TPL through its EAR motif (DLELRL). ( A ) Yeast two-hybrid assays testing interaction between TPL [fused to the <t>GAL4</t> DNA-binding domain (DBD)] and wild-type SUP (DLELRL), mSUP (DLE A R A ) or mSUP (DLE G R G ) [each fused to the <t>GAL4</t> <t>activation</t> <t>domain</t> (AD)]. Growth on selection (right panel) detects a physical interaction between TPL and wild-type SUP (DLELRL) only. ( B ) In vitro pull-down assays using GST-tagged N-TPL show a strong interaction with SBP-tagged wild-type SUP (DLELRL) that is weakened by mutation of the EAR motif in SUP (DLE A R A ). Ponceau S staining shows efficient GST protein expression. Full-length blots and quantification of Western blot signal intensities are presented in Supplementary Fig. S3](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5690/pmc12595690/pmc12595690__12870_2025_7553_Fig2_HTML.jpg)
![CRISPR-generated EAR mutations disrupt physical interaction between SUP and TPL. ( A ) Yeast two-hybrid assays testing interaction between TPL [fused to the GAL4 DNA-binding domain (DBD)] and wild-type SUP (DLELRL), mSUP (DLELL) or mSUP (∆EAR) [each fused to the GAL4 activation domain (AD)]. Growth on selection (right panel) indicates physical interaction between TPL and wild-type SUP (DLELRL) only. ( B ) In vitro pull-down assays using GST-tagged N-TPL show a strong interaction with SBP-tagged wild-type SUP (DLELRL) which is weakened by mutation of the EAR motif in CRISPR allele mSUP (DLELL). Ponceau S staining shows efficient GST protein expression. Full-length blots and quantification of Western blot signal intensities are presented in Supplementary Fig. S7](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5690/pmc12595690/pmc12595690__12870_2025_7553_Fig5_HTML.jpg)