antigal4 ad sc 1663 antibodies (Santa Cruz Biotechnology)
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antigal4 ad sc 1663 antibodies
Antigal4 Ad Sc 1663 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1663/GAL4-TA+Antibody/pm41204088-76-28-32
Average 94 stars, based on 65 article reviews
Antigal4 Ad Sc 1663 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/1663/GAL4-TA+Antibody/pm41204088-76-28-32
Average 94 stars, based on 65 article reviews
antigal4 ad sc 1663 antibodies - by Bioz Stars,
2026-11
94/100 stars
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Immunoprecipitation:Article Title: Rapid Examination of Phytochrome–Phytochrome Interacting Factor (PIF) Interaction by In Vitro Coimmunoprecipitation Assay Article Snippet: Typhoon imaging system (GE Healthcare Life Sciences). .. Antibody for the immunoprecipitation of the bait protein; we used Article Title: Rapid Examination of Phytochrome–Phytochrome Interacting Factor (PIF) Interaction by In Vitro Coimmunoprecipitation Assay Article Snippet: Typhoon imaging system (GE Healthcare Life Sciences). .. Coimmunoprecipitation Antibody for the immunoprecipitation of the bait protein; we used Expressing:Article Title: SUPERMAN requires its EAR motif to recruit the corepressor TOPLESS and to function as a cadastral regulator in floral patterning. Article Snippet: .. To verify yeast protein expression, Western blots were stained with 0.1% Ponceau S in 5% v/v acetic acid, washed in water, and analyzed using anti-GAL4 DBD (sc-577) or Western Blot:Article Title: SUPERMAN requires its EAR motif to recruit the corepressor TOPLESS and to function as a cadastral regulator in floral patterning. Article Snippet: .. To verify yeast protein expression, Western blots were stained with 0.1% Ponceau S in 5% v/v acetic acid, washed in water, and analyzed using anti-GAL4 DBD (sc-577) or Article Title: The FAM104 proteins VCF1/2 promote the nuclear localization of p97/VCP Article Snippet: Antibody , Anti-alpha-tubulin (mouse monoclonal) , Sigma-Aldrich , Cat# T5168, RRID: AB_477579 , , WB 1:2500. .. Antibody , Anti-GAL4-TA (mouse monoclonal) , Santa Cruz , Sc-1663 , , WB 1:500. .. Antibody , Anti-FLAG (rabbit polyclonal) , Thermo Fisher Scientific , Cat# PA1-984B, RRID: AB_347227 , , WB 1:2000. Staining:Article Title: SUPERMAN requires its EAR motif to recruit the corepressor TOPLESS and to function as a cadastral regulator in floral patterning. Article Snippet: .. To verify yeast protein expression, Western blots were stained with 0.1% Ponceau S in 5% v/v acetic acid, washed in water, and analyzed using anti-GAL4 DBD (sc-577) or other:Article Title: LHP1 and INO80 cooperate with ethylene signaling for warm ambient temperature response by activating specific bivalent genes Article Snippet: Article Title: Subcellular targets and recognition mechanism of Ralstonia solanacearum effector RipE1 Article Snippet: anti-GAL4-DBD , |
![SUP physically interacts with TPL through its EAR motif (DLELRL). ( A ) Yeast two-hybrid assays testing interaction between TPL [fused to the <t>GAL4</t> DNA-binding domain (DBD)] and wild-type SUP (DLELRL), mSUP (DLE A R A ) or mSUP (DLE G R G ) [each fused to the <t>GAL4</t> <t>activation</t> <t>domain</t> (AD)]. Growth on selection (right panel) detects a physical interaction between TPL and wild-type SUP (DLELRL) only. ( B ) In vitro pull-down assays using GST-tagged N-TPL show a strong interaction with SBP-tagged wild-type SUP (DLELRL) that is weakened by mutation of the EAR motif in SUP (DLE A R A ). Ponceau S staining shows efficient GST protein expression. Full-length blots and quantification of Western blot signal intensities are presented in Supplementary Fig. S3](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5690/pmc12595690/pmc12595690__12870_2025_7553_Fig2_HTML.jpg)
![CRISPR-generated EAR mutations disrupt physical interaction between SUP and TPL. ( A ) Yeast two-hybrid assays testing interaction between TPL [fused to the GAL4 DNA-binding domain (DBD)] and wild-type SUP (DLELRL), mSUP (DLELL) or mSUP (∆EAR) [each fused to the GAL4 activation domain (AD)]. Growth on selection (right panel) indicates physical interaction between TPL and wild-type SUP (DLELRL) only. ( B ) In vitro pull-down assays using GST-tagged N-TPL show a strong interaction with SBP-tagged wild-type SUP (DLELRL) which is weakened by mutation of the EAR motif in CRISPR allele mSUP (DLELL). Ponceau S staining shows efficient GST protein expression. Full-length blots and quantification of Western blot signal intensities are presented in Supplementary Fig. S7](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5690/pmc12595690/pmc12595690__12870_2025_7553_Fig5_HTML.jpg)