Journal: Scientific Reports

Article Title: Sesamin alleviates androgenetic alopecia by targeting the AR MAPK Wnt axis

doi: 10.1038/s41598-025-32856-4

Figure Lengend Snippet: Western blot analysis and quantitative assessment of protein expression in HaCaT cells treated with Sesamin under DHT stimulation. ( A ) Western blot images showing the expression levels of Androgen Receptor ( AR ), Bcl-2 Associated X protein ( BAX ), B-cell CLL/lymphoma 2 ( BCL2 ), Wingless-type MMTV integration site family, member 10B ( WNT10B ), Glyceraldehyde-3-phosphate Dehydrogenase ( GAPDH ), P38 Mitogen-Activated Protein Kinase (P38), and Phospho-P38 Mitogen-Activated Protein Kinase (P-P38) across different treatment groups: Untreated (UT), DHT-treated (DHT), and DHT co-treated with 10 µM or 80 µM sesamin (10, 80). GAPDH was used as a loading control. Black lines indicate where the blots were cropped, and full-length blots are provided in Supplementary Figure . ( B ) Quantification of AR expression normalized to GAPDH. ( C ) Quantification of WNT-10B expression normalized to GAPDH. ( D ) Quantification of BAX expression normalized to GAPDH. ( E ) Quantification of BCL2 expression normalized to GAPDH. ( F ) Western blot images showing the expression levels of P38 and P-P38 proteins. ( G ) Quantification of P-P38 expression normalized to total P38. Data represent mean ± SD from three independent biological experiments ( n = 3). Statistical analysis was performed using one-way ANOVA with Dunnett’s multiple comparison test, which controls for multiple comparisons against a single control group. The symbols indicate comparisons to the UT group (#) or the DHT group (*). The symbols indicate the following: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Sesamin was purchased from Chengdu Aifa Biotechnology Co., Ltd.; Dulbecco’s modified eagle medium (DMEM) and fetal bovine serum were purchased from Saiaomei Cell Technology (Beijing) Co., Ltd.; dimethyl sulfoxide (DMSO) was purchased from BOSTER Biological Technology Co., Ltd.; BCA protein quantification kit was purchased from Beijing Bijie Biotechnology Co., Ltd.; MTT kit was purchased from Hangzhou Zeheng Biotechnology Co., Ltd., RIPA lysate (medium), protease inhibitor, phosphatase inhibitor were purchased from Thermo Fisher Scientific; SDS-PAGE gel preparation kit was purchased from Hangzhou Dacheng Biotechnology Co. Primary antibodies against AR , p38, phospho-p38, BAX , BCL2 , WNT10B , and GAPDH were purchased from CST Co., Ltd.; goat anti-mouse IgG, goat anti-rabbit IgG were purchased from BOSTER Biological Technology Co.

Techniques: Western Blot, Expressing, Control, Comparison

Journal: Nature Communications

Article Title: Activation of canonical Wnt signalling is required for TGF-β-mediated fibrosis

doi: 10.1038/ncomms1734

Figure Lengend Snippet: ( a ) Confocal microscopy with double staining for Wnt-1 and vimentin demonstrated an overexpression of Wnt-1, in particular, in vimentin-positive cells in fibrotic skin of patients with SSc, in IPF and in liver cirrhosis. ( b ) Wnt-10b was also overexpressed in SSc, IPF and liver cirrhosis. ( c ) In parallel to the upregulation of Wnt-1 and Wnt-10b, a prominent decrease of the endogenous antagonist Dkk-1 was observed in fibroblasts in fibrotic diseases. Representative examples from 12 patients with SSc, 10 samples of non-fibrotic skin, 10 patients with IPF, 6 control lungs, 8 patients with liver cirrhosis and 8 control livers are shown. Scale bars, 20 or 5 μm for the outer right image of each panel, respectively.

Article Snippet: Formalin-fixed, paraffin-embedded skin sections were stained with anti-β-catenin antibodies (R&D Systems), anti-αSMA antibodies (Sigma-Aldrich), anti-Wnt-1 antibodies (Abcam, Cambridge, UK), anti-Wnt-10b antibodies (ProSci Incorporated, Poway, CA, USA), anti-murine Dkk-1 (R&D Systems) and anti-human Dkk-1 (R&D Systems).

Techniques: Confocal Microscopy, Double Staining, Over Expression, Control

Journal: Nature Communications

Article Title: Activation of canonical Wnt signalling is required for TGF-β-mediated fibrosis

doi: 10.1038/ncomms1734

Figure Lengend Snippet: ( a ) Recombinant Wnt-1 stimulated the activity of col1a2 promoter constructs ( n =6). ( b ) Incubation with recombinant Wnt-1 increased the release of collagen protein by cultured fibroblasts in a dose-dependent manner ( n =6). ( c ) Wnt-1 induced the expression of αSMA protein ( n =5). ( d ) Incubation with Wnt-1 increased the mRNA levels of αSMA ( n =5). ( e ) Wnt-1 stimulated the formation of stress fibres in cultured fibroblasts ( n =6). Of note, the stimulatory effects of Wnt-1 on fibroblasts were comparable with those of TGF-β. ( f ) Transgenic overexpression of Wnt-10b induced dermal fibrosis in mice. The dermal thickness, the hydroxyproline content and the numbers of myofibroblasts were already increased in Wnt-10b tg mice at an age of 3 weeks and increased further with age ( n ≥ 5 for all groups). Representative haematoxylin and eosin-, trichrome- and Sirius Red-stained sections of wild-type (WT) and Wnt-10b transgenic (Wnt-10b tg) mice at ages of 3, 6 and 12 weeks are shown (horizontal scale bars, 100 μm). Black vertical bars indicate the dermal thickness. * Indicates P -values of less than 0.05 as compared with mock-treated fibroblasts ( a – e , analysed with the Wilcoxon signed-rank test) or compared with wild-type littermates ( f , analysed using the Mann–Whitney U -test), respectively. All data are expressed as mean ± s.e.m.

Article Snippet: Formalin-fixed, paraffin-embedded skin sections were stained with anti-β-catenin antibodies (R&D Systems), anti-αSMA antibodies (Sigma-Aldrich), anti-Wnt-1 antibodies (Abcam, Cambridge, UK), anti-Wnt-10b antibodies (ProSci Incorporated, Poway, CA, USA), anti-murine Dkk-1 (R&D Systems) and anti-human Dkk-1 (R&D Systems).

Techniques: Recombinant, Activity Assay, Construct, Incubation, Cell Culture, Expressing, Transgenic Assay, Over Expression, Staining, MANN-WHITNEY