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Image Search Results
Journal: Nature Communications
Article Title: Tracking down the molecular architecture of the synaptonemal complex by expansion microscopy
doi: 10.1038/s41467-020-17017-7
Figure Lengend Snippet: a SIM image of post-expansion SeTau647 labeled SYCP3 as a component of the lateral element (red), the transverse filament SYCP1 N terminus labeled with Alexa Fluor 488 (green), and SYCE3 of the central element labeled with Alexa Fluor 568 (magenta). b Magnified views of white boxed region in ( a ). ii Orthogonal view of horizontal white dashed line in i. iii Orthogonal view of vertical white dashed line in ( i ). c Transversal intensity profile perpendicular to the orientation of the SC shown in ( b ). The selected section exhibits a bimodal distribution of the SYCP3 signal separated by 667.0 nm ± 7.1 nm (SD). The SYCP1 N terminus and SYCE3 signals of the section ( b ) show monomodal distributions with FWHM of 214.7 ± 6.9 nm (SD) and 258.3 ± 4.8 nm (SD), respectively. d Large field of view (100 × 100 × 30 µm ) 3D-re-scan confocal microscopy image of spreadings. SYCP3 was labeled post-expansion with SeTau647. e Magnified view of boxed region in ( d ). ( f ) 3D-MAP-SIM image of the SCs of an entire set of chromosomes in a spermatocyte visualized by post-expansion labeling of SYCP3 with SeTau647. The inlet shows the enlarged view of the boxed region in ( f ). Scale bars, ( a ) 10 µm, ( b ), i–iii 1 µm, ( d ) 25 µm, ( e ) 10 µm, ( f ) 15 µm, ( f ), inlet 5 µm.
Article Snippet: 3D visualization of MAP-SIM data: 3D-MAP-SIM data shown in Supplementary Movie and Supplementary Movie were visualized using the
Techniques: Labeling, Confocal Microscopy