vector pcdna3 1 gfp Search Results


gfp vector  (Addgene inc)
96
Addgene inc gfp vector
A. Control (NC) and BRAT1 knockdown (S15 and S17) Hela cells were cultured in 10% FBS/DMEM (media) or serum-free DMEM for 24 h (SS), followed by 10% complete DMEM for 2 h (SSF). Total lysates were isolated and subjected to immunoblotting with indicated antibodies. Actin was used as internal control to validate protein loading. B. Mouse embryonic fibroblasts (MEFs) were isolated from E15 of BRAT1flox/flox female mouse as described in Materials and Methods. Cells were transfected with <t>GFP</t> only (V) or Cre-GFP plasmid (CreG). C. Immortalized MEFs infected with pBabe retrovirus (pbabe) or Cre gene retrovirus (Cre) were transfected with BRAT-GST plasmid (+) or GST vector plasmid (−). After 48 hour, Total lysates were subjected to immunoblot. D. 293 cells were transfected <t>with</t> <t>plasmids</t> expressing GST-fusion fragments (#3, 5, 6) or full-length (FL) of BRAT1. Total lysates (1 mg/sample) were subjected to immunoprecipitation with GSH-sepharose beads. After immunoprecipitation, samples were blotted with the indicated antibodies. E. Control (pbabe) and BRAT1 knockout (Cre) MEFs were culture in plain DMEM for 24 hr, and then re-cultured in 10% complete DMEM for 7 or 20 h. Cell cycle was quantified by single-parameter flow cytometry after PI staining. Graphs represent percentages of cells in sub-G1, G1, S, and G2/M phases. Data shown here are representative of three independent experiments performed.
Gfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pcdna3+1+gfp/pcDNA3%2E1(%2B)+Laccase2+MCS+Exon+Vector+(Plasmid+%2369893)/pmc04314932-51-6-11
Average 96 stars, based on 1 article reviews
gfp vector - by Bioz Stars, 2026-10
96/100 stars
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mammalian expression vectors pcdna3.1  (GenScript corporation)
90
GenScript corporation mammalian expression vectors pcdna3.1
A. Control (NC) and BRAT1 knockdown (S15 and S17) Hela cells were cultured in 10% FBS/DMEM (media) or serum-free DMEM for 24 h (SS), followed by 10% complete DMEM for 2 h (SSF). Total lysates were isolated and subjected to immunoblotting with indicated antibodies. Actin was used as internal control to validate protein loading. B. Mouse embryonic fibroblasts (MEFs) were isolated from E15 of BRAT1flox/flox female mouse as described in Materials and Methods. Cells were transfected with <t>GFP</t> only (V) or Cre-GFP plasmid (CreG). C. Immortalized MEFs infected with pBabe retrovirus (pbabe) or Cre gene retrovirus (Cre) were transfected with BRAT-GST plasmid (+) or GST vector plasmid (−). After 48 hour, Total lysates were subjected to immunoblot. D. 293 cells were transfected <t>with</t> <t>plasmids</t> expressing GST-fusion fragments (#3, 5, 6) or full-length (FL) of BRAT1. Total lysates (1 mg/sample) were subjected to immunoprecipitation with GSH-sepharose beads. After immunoprecipitation, samples were blotted with the indicated antibodies. E. Control (pbabe) and BRAT1 knockout (Cre) MEFs were culture in plain DMEM for 24 hr, and then re-cultured in 10% complete DMEM for 7 or 20 h. Cell cycle was quantified by single-parameter flow cytometry after PI staining. Graphs represent percentages of cells in sub-G1, G1, S, and G2/M phases. Data shown here are representative of three independent experiments performed.
Mammalian Expression Vectors Pcdna3.1, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pcdna3+1+gfp/pcdna3+1/us11174308-95-14-53
Average 90 stars, based on 1 article reviews
mammalian expression vectors pcdna3.1 - by Bioz Stars, 2026-10
90/100 stars
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pcdna3 1 wnt7a gfp  (Addgene inc)
94
Addgene inc pcdna3 1 wnt7a gfp
A. Control (NC) and BRAT1 knockdown (S15 and S17) Hela cells were cultured in 10% FBS/DMEM (media) or serum-free DMEM for 24 h (SS), followed by 10% complete DMEM for 2 h (SSF). Total lysates were isolated and subjected to immunoblotting with indicated antibodies. Actin was used as internal control to validate protein loading. B. Mouse embryonic fibroblasts (MEFs) were isolated from E15 of BRAT1flox/flox female mouse as described in Materials and Methods. Cells were transfected with <t>GFP</t> only (V) or Cre-GFP plasmid (CreG). C. Immortalized MEFs infected with pBabe retrovirus (pbabe) or Cre gene retrovirus (Cre) were transfected with BRAT-GST plasmid (+) or GST vector plasmid (−). After 48 hour, Total lysates were subjected to immunoblot. D. 293 cells were transfected <t>with</t> <t>plasmids</t> expressing GST-fusion fragments (#3, 5, 6) or full-length (FL) of BRAT1. Total lysates (1 mg/sample) were subjected to immunoprecipitation with GSH-sepharose beads. After immunoprecipitation, samples were blotted with the indicated antibodies. E. Control (pbabe) and BRAT1 knockout (Cre) MEFs were culture in plain DMEM for 24 hr, and then re-cultured in 10% complete DMEM for 7 or 20 h. Cell cycle was quantified by single-parameter flow cytometry after PI staining. Graphs represent percentages of cells in sub-G1, G1, S, and G2/M phases. Data shown here are representative of three independent experiments performed.
Pcdna3 1 Wnt7a Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pcdna3+1+gfp/pcDNA3+GFP+GFP+(Plasmid+%2320738)/pmc11658684-179-16-18
Average 94 stars, based on 1 article reviews
pcdna3 1 wnt7a gfp - by Bioz Stars, 2026-10
94/100 stars
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pcdh gfp serping1 plasmid  (Addgene inc)
95
Addgene inc pcdh gfp serping1 plasmid
A. Control (NC) and BRAT1 knockdown (S15 and S17) Hela cells were cultured in 10% FBS/DMEM (media) or serum-free DMEM for 24 h (SS), followed by 10% complete DMEM for 2 h (SSF). Total lysates were isolated and subjected to immunoblotting with indicated antibodies. Actin was used as internal control to validate protein loading. B. Mouse embryonic fibroblasts (MEFs) were isolated from E15 of BRAT1flox/flox female mouse as described in Materials and Methods. Cells were transfected with <t>GFP</t> only (V) or Cre-GFP plasmid (CreG). C. Immortalized MEFs infected with pBabe retrovirus (pbabe) or Cre gene retrovirus (Cre) were transfected with BRAT-GST plasmid (+) or GST vector plasmid (−). After 48 hour, Total lysates were subjected to immunoblot. D. 293 cells were transfected <t>with</t> <t>plasmids</t> expressing GST-fusion fragments (#3, 5, 6) or full-length (FL) of BRAT1. Total lysates (1 mg/sample) were subjected to immunoprecipitation with GSH-sepharose beads. After immunoprecipitation, samples were blotted with the indicated antibodies. E. Control (pbabe) and BRAT1 knockout (Cre) MEFs were culture in plain DMEM for 24 hr, and then re-cultured in 10% complete DMEM for 7 or 20 h. Cell cycle was quantified by single-parameter flow cytometry after PI staining. Graphs represent percentages of cells in sub-G1, G1, S, and G2/M phases. Data shown here are representative of three independent experiments performed.
Pcdh Gfp Serping1 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pcdna3+1+gfp/pcDNA3%2E1-HA+(Plasmid+%23128034)/pmc09379417-32-16-14
Average 95 stars, based on 1 article reviews
pcdh gfp serping1 plasmid - by Bioz Stars, 2026-10
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pca ires gfp vector  (Addgene inc)
93
Addgene inc pca ires gfp vector
A. Control (NC) and BRAT1 knockdown (S15 and S17) Hela cells were cultured in 10% FBS/DMEM (media) or serum-free DMEM for 24 h (SS), followed by 10% complete DMEM for 2 h (SSF). Total lysates were isolated and subjected to immunoblotting with indicated antibodies. Actin was used as internal control to validate protein loading. B. Mouse embryonic fibroblasts (MEFs) were isolated from E15 of BRAT1flox/flox female mouse as described in Materials and Methods. Cells were transfected with <t>GFP</t> only (V) or Cre-GFP plasmid (CreG). C. Immortalized MEFs infected with pBabe retrovirus (pbabe) or Cre gene retrovirus (Cre) were transfected with BRAT-GST plasmid (+) or GST vector plasmid (−). After 48 hour, Total lysates were subjected to immunoblot. D. 293 cells were transfected <t>with</t> <t>plasmids</t> expressing GST-fusion fragments (#3, 5, 6) or full-length (FL) of BRAT1. Total lysates (1 mg/sample) were subjected to immunoprecipitation with GSH-sepharose beads. After immunoprecipitation, samples were blotted with the indicated antibodies. E. Control (pbabe) and BRAT1 knockout (Cre) MEFs were culture in plain DMEM for 24 hr, and then re-cultured in 10% complete DMEM for 7 or 20 h. Cell cycle was quantified by single-parameter flow cytometry after PI staining. Graphs represent percentages of cells in sub-G1, G1, S, and G2/M phases. Data shown here are representative of three independent experiments performed.
Pca Ires Gfp Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pcdna3+1+gfp/pcDNA3%2E1(%2B)IRES+GFP+(Plasmid+%2351406)/bio_rxiv__690263-186-5-19
Average 93 stars, based on 1 article reviews
pca ires gfp vector - by Bioz Stars, 2026-10
93/100 stars
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gfp targeting vector  (Addgene inc)
96
Addgene inc gfp targeting vector
A. Control (NC) and BRAT1 knockdown (S15 and S17) Hela cells were cultured in 10% FBS/DMEM (media) or serum-free DMEM for 24 h (SS), followed by 10% complete DMEM for 2 h (SSF). Total lysates were isolated and subjected to immunoblotting with indicated antibodies. Actin was used as internal control to validate protein loading. B. Mouse embryonic fibroblasts (MEFs) were isolated from E15 of BRAT1flox/flox female mouse as described in Materials and Methods. Cells were transfected with <t>GFP</t> only (V) or Cre-GFP plasmid (CreG). C. Immortalized MEFs infected with pBabe retrovirus (pbabe) or Cre gene retrovirus (Cre) were transfected with BRAT-GST plasmid (+) or GST vector plasmid (−). After 48 hour, Total lysates were subjected to immunoblot. D. 293 cells were transfected <t>with</t> <t>plasmids</t> expressing GST-fusion fragments (#3, 5, 6) or full-length (FL) of BRAT1. Total lysates (1 mg/sample) were subjected to immunoprecipitation with GSH-sepharose beads. After immunoprecipitation, samples were blotted with the indicated antibodies. E. Control (pbabe) and BRAT1 knockout (Cre) MEFs were culture in plain DMEM for 24 hr, and then re-cultured in 10% complete DMEM for 7 or 20 h. Cell cycle was quantified by single-parameter flow cytometry after PI staining. Graphs represent percentages of cells in sub-G1, G1, S, and G2/M phases. Data shown here are representative of three independent experiments performed.
Gfp Targeting Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pcdna3+1+gfp/pcDNA3%2E1(%2B)+CircRNA+Mini+Vector+(Plasmid+%2360648)/pmc02801346-120-3-11
Average 96 stars, based on 1 article reviews
gfp targeting vector - by Bioz Stars, 2026-10
96/100 stars
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pcdna3 1 lic 6d  (Addgene inc)
93
Addgene inc pcdna3 1 lic 6d
A. Control (NC) and BRAT1 knockdown (S15 and S17) Hela cells were cultured in 10% FBS/DMEM (media) or serum-free DMEM for 24 h (SS), followed by 10% complete DMEM for 2 h (SSF). Total lysates were isolated and subjected to immunoblotting with indicated antibodies. Actin was used as internal control to validate protein loading. B. Mouse embryonic fibroblasts (MEFs) were isolated from E15 of BRAT1flox/flox female mouse as described in Materials and Methods. Cells were transfected with <t>GFP</t> only (V) or Cre-GFP plasmid (CreG). C. Immortalized MEFs infected with pBabe retrovirus (pbabe) or Cre gene retrovirus (Cre) were transfected with BRAT-GST plasmid (+) or GST vector plasmid (−). After 48 hour, Total lysates were subjected to immunoblot. D. 293 cells were transfected <t>with</t> <t>plasmids</t> expressing GST-fusion fragments (#3, 5, 6) or full-length (FL) of BRAT1. Total lysates (1 mg/sample) were subjected to immunoprecipitation with GSH-sepharose beads. After immunoprecipitation, samples were blotted with the indicated antibodies. E. Control (pbabe) and BRAT1 knockout (Cre) MEFs were culture in plain DMEM for 24 hr, and then re-cultured in 10% complete DMEM for 7 or 20 h. Cell cycle was quantified by single-parameter flow cytometry after PI staining. Graphs represent percentages of cells in sub-G1, G1, S, and G2/M phases. Data shown here are representative of three independent experiments performed.
Pcdna3 1 Lic 6d, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pcdna3+1+gfp/pcDNA3+GFP+LIC+cloning+vector+(6D)+(Plasmid+%2330127)/pm34019797-280-24-27
Average 93 stars, based on 1 article reviews
pcdna3 1 lic 6d - by Bioz Stars, 2026-10
93/100 stars
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pet 30a vector novagen  (Addgene inc)
93
Addgene inc pet 30a vector novagen
A. Control (NC) and BRAT1 knockdown (S15 and S17) Hela cells were cultured in 10% FBS/DMEM (media) or serum-free DMEM for 24 h (SS), followed by 10% complete DMEM for 2 h (SSF). Total lysates were isolated and subjected to immunoblotting with indicated antibodies. Actin was used as internal control to validate protein loading. B. Mouse embryonic fibroblasts (MEFs) were isolated from E15 of BRAT1flox/flox female mouse as described in Materials and Methods. Cells were transfected with <t>GFP</t> only (V) or Cre-GFP plasmid (CreG). C. Immortalized MEFs infected with pBabe retrovirus (pbabe) or Cre gene retrovirus (Cre) were transfected with BRAT-GST plasmid (+) or GST vector plasmid (−). After 48 hour, Total lysates were subjected to immunoblot. D. 293 cells were transfected <t>with</t> <t>plasmids</t> expressing GST-fusion fragments (#3, 5, 6) or full-length (FL) of BRAT1. Total lysates (1 mg/sample) were subjected to immunoprecipitation with GSH-sepharose beads. After immunoprecipitation, samples were blotted with the indicated antibodies. E. Control (pbabe) and BRAT1 knockout (Cre) MEFs were culture in plain DMEM for 24 hr, and then re-cultured in 10% complete DMEM for 7 or 20 h. Cell cycle was quantified by single-parameter flow cytometry after PI staining. Graphs represent percentages of cells in sub-G1, G1, S, and G2/M phases. Data shown here are representative of three independent experiments performed.
Pet 30a Vector Novagen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pcdna3+1+gfp/pcDNA3%2E1(%2B)+ZKSCAN1+MCS-WT+Split+GFP+%2B+Sense+IRES+(Plasmid+%2369909)/pm40570374-483-180-196
Average 93 stars, based on 1 article reviews
pet 30a vector novagen - by Bioz Stars, 2026-10
93/100 stars
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pken mut 2 vector  (Addgene inc)
90
Addgene inc pken mut 2 vector
A. Control (NC) and BRAT1 knockdown (S15 and S17) Hela cells were cultured in 10% FBS/DMEM (media) or serum-free DMEM for 24 h (SS), followed by 10% complete DMEM for 2 h (SSF). Total lysates were isolated and subjected to immunoblotting with indicated antibodies. Actin was used as internal control to validate protein loading. B. Mouse embryonic fibroblasts (MEFs) were isolated from E15 of BRAT1flox/flox female mouse as described in Materials and Methods. Cells were transfected with <t>GFP</t> only (V) or Cre-GFP plasmid (CreG). C. Immortalized MEFs infected with pBabe retrovirus (pbabe) or Cre gene retrovirus (Cre) were transfected with BRAT-GST plasmid (+) or GST vector plasmid (−). After 48 hour, Total lysates were subjected to immunoblot. D. 293 cells were transfected <t>with</t> <t>plasmids</t> expressing GST-fusion fragments (#3, 5, 6) or full-length (FL) of BRAT1. Total lysates (1 mg/sample) were subjected to immunoprecipitation with GSH-sepharose beads. After immunoprecipitation, samples were blotted with the indicated antibodies. E. Control (pbabe) and BRAT1 knockout (Cre) MEFs were culture in plain DMEM for 24 hr, and then re-cultured in 10% complete DMEM for 7 or 20 h. Cell cycle was quantified by single-parameter flow cytometry after PI staining. Graphs represent percentages of cells in sub-G1, G1, S, and G2/M phases. Data shown here are representative of three independent experiments performed.
Pken Mut 2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pcdna3+1+gfp/pKEN+GFP+mut2+(Plasmid+%2320409)/pmc06007110-399-7-11
Average 90 stars, based on 1 article reviews
pken mut 2 vector - by Bioz Stars, 2026-10
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pad5 vector  (Addgene inc)
94
Addgene inc pad5 vector

Pad5 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pcdna3+1+gfp/pWPT-GFP+(Plasmid+%2312255)/pmc08449092-70-0-3
Average 94 stars, based on 1 article reviews
pad5 vector - by Bioz Stars, 2026-10
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pcdna3.1-gfp expression plasmid  (Promega)
90
Promega pcdna3.1-gfp expression plasmid

Pcdna3.1 Gfp Expression Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pcdna3+1+gfp/pcdna3+1/10__1074_slash_jbc__m117__775080-263-18-27
Average 90 stars, based on 1 article reviews
pcdna3.1-gfp expression plasmid - by Bioz Stars, 2026-10
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eukaryotic expression vector pcdna3 1 gfp  (New England Biolabs)
99
New England Biolabs eukaryotic expression vector pcdna3 1 gfp

Eukaryotic Expression Vector Pcdna3 1 Gfp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vector+pcdna3+1+gfp/EcoRI/pm29742499-66-24-35
Average 99 stars, based on 1 article reviews
eukaryotic expression vector pcdna3 1 gfp - by Bioz Stars, 2026-10
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Image Search Results


A. Control (NC) and BRAT1 knockdown (S15 and S17) Hela cells were cultured in 10% FBS/DMEM (media) or serum-free DMEM for 24 h (SS), followed by 10% complete DMEM for 2 h (SSF). Total lysates were isolated and subjected to immunoblotting with indicated antibodies. Actin was used as internal control to validate protein loading. B. Mouse embryonic fibroblasts (MEFs) were isolated from E15 of BRAT1flox/flox female mouse as described in Materials and Methods. Cells were transfected with GFP only (V) or Cre-GFP plasmid (CreG). C. Immortalized MEFs infected with pBabe retrovirus (pbabe) or Cre gene retrovirus (Cre) were transfected with BRAT-GST plasmid (+) or GST vector plasmid (−). After 48 hour, Total lysates were subjected to immunoblot. D. 293 cells were transfected with plasmids expressing GST-fusion fragments (#3, 5, 6) or full-length (FL) of BRAT1. Total lysates (1 mg/sample) were subjected to immunoprecipitation with GSH-sepharose beads. After immunoprecipitation, samples were blotted with the indicated antibodies. E. Control (pbabe) and BRAT1 knockout (Cre) MEFs were culture in plain DMEM for 24 hr, and then re-cultured in 10% complete DMEM for 7 or 20 h. Cell cycle was quantified by single-parameter flow cytometry after PI staining. Graphs represent percentages of cells in sub-G1, G1, S, and G2/M phases. Data shown here are representative of three independent experiments performed.

Journal: Journal of cancer biology & research

Article Title: The Potential Role of BRCA1-Associated ATM Activator-1 (BRAT1) in Regulation of mTOR

doi:

Figure Lengend Snippet: A. Control (NC) and BRAT1 knockdown (S15 and S17) Hela cells were cultured in 10% FBS/DMEM (media) or serum-free DMEM for 24 h (SS), followed by 10% complete DMEM for 2 h (SSF). Total lysates were isolated and subjected to immunoblotting with indicated antibodies. Actin was used as internal control to validate protein loading. B. Mouse embryonic fibroblasts (MEFs) were isolated from E15 of BRAT1flox/flox female mouse as described in Materials and Methods. Cells were transfected with GFP only (V) or Cre-GFP plasmid (CreG). C. Immortalized MEFs infected with pBabe retrovirus (pbabe) or Cre gene retrovirus (Cre) were transfected with BRAT-GST plasmid (+) or GST vector plasmid (−). After 48 hour, Total lysates were subjected to immunoblot. D. 293 cells were transfected with plasmids expressing GST-fusion fragments (#3, 5, 6) or full-length (FL) of BRAT1. Total lysates (1 mg/sample) were subjected to immunoprecipitation with GSH-sepharose beads. After immunoprecipitation, samples were blotted with the indicated antibodies. E. Control (pbabe) and BRAT1 knockout (Cre) MEFs were culture in plain DMEM for 24 hr, and then re-cultured in 10% complete DMEM for 7 or 20 h. Cell cycle was quantified by single-parameter flow cytometry after PI staining. Graphs represent percentages of cells in sub-G1, G1, S, and G2/M phases. Data shown here are representative of three independent experiments performed.

Article Snippet: Plasmids containing Cre gene (pCAG-Cre:GFP) and GFP vector were provided from Addgene (Cambridge, MA), and used to target BRAT1 gene, transiently.

Techniques: Cell Culture, Isolation, Western Blot, Transfection, Plasmid Preparation, Infection, Expressing, Immunoprecipitation, Knock-Out, Flow Cytometry, Staining

Journal: iScience

Article Title: Loss of miR-29a impairs decidualization of endometrial stromal cells by TET3 mediated demethylation of Col1A1 promoter

doi: 10.1016/j.isci.2021.103065

Figure Lengend Snippet:

Article Snippet: pAd5 vector , Addgene , Cat#122555.

Techniques: Recombinant, Lysis, Marker, SYBR Green Assay, Plasmid Preparation, CCK-8 Assay, Bicinchoninic Acid Protein Assay, Luciferase, Software