Journal: Vaccines

Article Title: Pleurocidin Peptide Enhances Grouper Anti- Vibrio harveyi Immunity Elicited by Poly(lactide-co-glycolide)-Encapsulated Recombinant Glyceraldehyde-3-phosphate Dehydrogenase

doi: 10.3390/vaccines2020380

Figure Lengend Snippet: Survival of immunized grouper after a lethal V. harveyi challenge. Grouper were intraperitoneally immunized twice with PLG-PLE/rGAPDH microparticles (■), PLG-rGAPDH microparticles (●), rGAPDH alone (□) or PBS (○). Nine weeks after boosting, four groups of 60 groupers each were intraperitoneally infected with 6 × 10 6 CFU of V. harveyi (BCRC13812 strain). Fish were observed daily for an additional month (28 days), and the final survival rates were calculated. * p < 0.05 when comparing the PLG-PLE/rGAPDH group to the PLG-rGAPDH group.

Article Snippet: The V. harveyi BCRC13812 strain was purchased from the Bioresource Collection and Research Center (BCRC), Hsinchu, Taiwan, and was grown in trypticase soy broth (TSB, Difco) with 2% NaCl at 25 °C for 18 h. A total of 1 × 10 8 bacteria was incubated with lysis buffer (1% sodium dodecyl sulfate, 10 mM Tris-HCl (pH 8.0), 10 mM EDTA, 40 mM NaCl and 100 μg/mL proteinase K) for 1 h at 37 °C, and their DNA was extracted by phenol-chloroform extraction and ethanol precipitation, as described previously [ ].

Techniques: Infection

Journal: Biosensors

Article Title: Enhanced Detection of Vibrio harveyi Using a Dual-Composite DNAzyme-Based Biosensor

doi: 10.3390/bios14110548

Figure Lengend Snippet: Sensitivity of DVh 3+1 : ( a ) fluorescence intensity signals of different concentrations of V. harveyi (Blank: normal saline) and the calibration curves constructed using the fluorescence signals corresponding to 4.7 × 10 1 , 4.7 × 10 2 , and 4.7 × 10 3 CFU/mL of V. harveyi ; and ( b ) gel cleavage assay at different concentrations of V. harveyi (Blank: reaction system without CEM-Vh).

Article Snippet: The DNA (with a specific structure), combined with CEM of V. harveyi (CEM-Vh), could then undergo cleavage reaction, and the result was used in the next round of screening.

Techniques: Fluorescence, Saline, Construct, Cleavage Assay

Journal: Microorganisms

Article Title: Modulation of Campylobacter jejuni Motility, Adhesion to Polystyrene Surfaces, and Invasion of INT407 Cells by Quorum-Sensing Inhibition

doi: 10.3390/microorganisms8010104

Figure Lengend Snippet: Luminescence of V. harveyi BB170 cells (relative fluorescent units; RLU) after addition of C. jejuni 11168 cell-free supernatants from untreated C. jejuni cultures (control, empty bar) and C. jejuni cultures treated with essential oils (EO1–6), ethanolic extracts (E1–11), and pure compounds (P1–3) (full bars). Data are means ± standard deviation. *, p < 0.05 versus control (Student’s t -tests).

Article Snippet: Inhibition of C. jejuni QS was examined using the AI-2 bioluminescence assays, where the C. jejuni CFSs, which contained the QS signals, were added to the biosensory strain V. harveyi BB170.

Techniques: Control, Standard Deviation