Journal: Biochimica et biophysica acta

Article Title: Increases in intracellular calcium perturb blood-brain barrier via protein kinase C-alpha and apoptosis.

doi: 10.1016/j.bbadis.2015.10.016

Figure Lengend Snippet: Fig. 4. Amiloride and apocynin reduce PKC activity and uPA activity affected by PKC-α. HBMEC were exposed to 4 h OGD (OGD) with or without reperfusion (R) in the absence or presence of inhibitors for uPA (amiloride), NADPH oxidase (apocynin), intracellular calcium (BAPTA-AM) or PKC-α (RO-32-0432). Amiloride and apocynin decreased the rise in total PKC (A) and PKC-α (B) activities seen during OGD ± R. tPA activity is increased during OGD ± R and treatment with BAPT-AM and RO-32-0432 show no effect (C). uPA activity is also increased during OGD ± R and BAPTA-AM and RO-32-0432 normalised the increases seen (D). Data represented as mean ± SEM from n ≥3.* P b 0.05 compared to Normoxia. † P b 0.05 compared to re- spective untreated group.

Article Snippet: PAI-1-bound active uPA or tPA was detected by anti-tPA or anti-uPA primary antibodies (1:50, Santa Cruz Biotechnology) and primary antibodies were probed with horseradish peroxidase-linked secondary antibodies (1:5000, Santa Cruz).

Techniques: Activity Assay