tom20 tomm20 Search Results


anti tomm20  (MedChemExpress)
94
MedChemExpress anti tomm20
Anti Tomm20, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tom20+tomm20/TOMM20+Antibody/pm34224750-213-9-5
Average 94 stars, based on 1 article reviews
anti tomm20 - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
tomm20 antibody  (Bio-Techne corporation)
94
Bio-Techne corporation tomm20 antibody
Tomm20 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tom20+tomm20/TOMM20+Antibody/custom%40nbp1-81556%4011436949
Average 94 stars, based on 1 article reviews
tomm20 antibody - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
rabbit anti tom20  (Proteintech)
96
Proteintech rabbit anti tom20
Rabbit Anti Tom20, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tom20+tomm20/TOM20+Antibody/10__4103_slash_nrr__nrr___d___24___01273-142-93-96
Average 96 stars, based on 1 article reviews
rabbit anti tom20 - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
tomm20  (OriGene)
90
OriGene tomm20
Tomm20, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tom20+tomm20/TOMM20+(NM_014765)+Human+Untagged+Clone/pmc03032883-136-3-14
Average 90 stars, based on 1 article reviews
tomm20 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
tom20  (OriGene)
92
OriGene tom20
Fig. 1. Histamine evokes transfer of Ca2+ from the ER to mitochondria through IP3Rs. (A) Wide-field fluorescence images of HeLa cells expressing <t>TOM20–</t> GFP (green) and Mito-R-GECO1 (pseudo-coloured in magenta); the overlay shows colocalization in white. Images are typical of four independent experiments. Manders’ coefficients for the fraction of Mito-R-GECO1 colocalizing with TOM20–GFP=0.88±0.07 (mean±s.d.), n=4. (B) HeLa cells expressing Mito-R-GECO1 (mitochondrial Ca2+ indicator) and loaded with Calbryte 520 (cytosolic Ca2+ indicator) were stimulated with histamine (100 μM) in Ca2+-free HBS. Fluorescence from the Ca2+-saturated indicators (Fmax) was determined by addition of CGP 37157 (CGP, 50 μM) to inhibit the mitochondrial Na+/Ca2+ exchanger and then ionomycin (Iono, 10 μM) with 20 mM CaCl2. Results (F/Fmax) show fluorescence recorded from an entire cell. Results are typical of at least ten experiments. (C) Summary results show peak changes in fluorescence (ΔF/Fmax) evoked by histamine (100 μM) alone, or after pre-incubation with FCCP (5 μM, 10 min). Results show values for individual cells and mean±s.d. from ten (control) or 15 cells (FCCP) from three independent experiments. ns, not significant, P>0.05; **P<0.01; one-way ANOVA with Tukey’s correction. (D) Western blots showing the effect of non-silencing (NS) siRNA or siRNA directed at MCU in HeLa cells. Protein loadings (μg/lane) and molecular mass markers (kDa) are shown. Results are typical of three independent experiments. MCU siRNA reduced MCU expression to 22±7% of its expression in cells treated with NS siRNA (mean±s.e.m., n=3). (E,F) Effects of histamine (100 μM) in Ca2+-free HBS on [Ca2+]c (Calbryte 520) (E) or [Ca2+]m (Mito-R-GECO1) (F) in single HeLa cells treated with NS or MCU siRNA. Results, reported as F/Fmax, are typical of 15–16 cells from three independent experiments. (G) Summary results show effects of histamine in HeLa cells treated with NS or MCU siRNA. Results (individual values, mean±s.d.) are from 16 (NS) or 15 cells (MCU siRNA) from three independent experiments. ns, not significant, P>0.05; **P<0.01; one-way ANOVA with Tukey’s correction.
Tom20, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tom20+tomm20/TOMM20+(NM_014765)+Human+Tagged+ORF+Clone/pm38786982-154-7-40
Average 92 stars, based on 1 article reviews
tom20 - by Bioz Stars, 2026-09
92/100 stars
  Buy from Supplier
anti tomm20  (Boster Bio)
94
Boster Bio anti tomm20
Fig. 1. Histamine evokes transfer of Ca2+ from the ER to mitochondria through IP3Rs. (A) Wide-field fluorescence images of HeLa cells expressing <t>TOM20–</t> GFP (green) and Mito-R-GECO1 (pseudo-coloured in magenta); the overlay shows colocalization in white. Images are typical of four independent experiments. Manders’ coefficients for the fraction of Mito-R-GECO1 colocalizing with TOM20–GFP=0.88±0.07 (mean±s.d.), n=4. (B) HeLa cells expressing Mito-R-GECO1 (mitochondrial Ca2+ indicator) and loaded with Calbryte 520 (cytosolic Ca2+ indicator) were stimulated with histamine (100 μM) in Ca2+-free HBS. Fluorescence from the Ca2+-saturated indicators (Fmax) was determined by addition of CGP 37157 (CGP, 50 μM) to inhibit the mitochondrial Na+/Ca2+ exchanger and then ionomycin (Iono, 10 μM) with 20 mM CaCl2. Results (F/Fmax) show fluorescence recorded from an entire cell. Results are typical of at least ten experiments. (C) Summary results show peak changes in fluorescence (ΔF/Fmax) evoked by histamine (100 μM) alone, or after pre-incubation with FCCP (5 μM, 10 min). Results show values for individual cells and mean±s.d. from ten (control) or 15 cells (FCCP) from three independent experiments. ns, not significant, P>0.05; **P<0.01; one-way ANOVA with Tukey’s correction. (D) Western blots showing the effect of non-silencing (NS) siRNA or siRNA directed at MCU in HeLa cells. Protein loadings (μg/lane) and molecular mass markers (kDa) are shown. Results are typical of three independent experiments. MCU siRNA reduced MCU expression to 22±7% of its expression in cells treated with NS siRNA (mean±s.e.m., n=3). (E,F) Effects of histamine (100 μM) in Ca2+-free HBS on [Ca2+]c (Calbryte 520) (E) or [Ca2+]m (Mito-R-GECO1) (F) in single HeLa cells treated with NS or MCU siRNA. Results, reported as F/Fmax, are typical of 15–16 cells from three independent experiments. (G) Summary results show effects of histamine in HeLa cells treated with NS or MCU siRNA. Results (individual values, mean±s.d.) are from 16 (NS) or 15 cells (MCU siRNA) from three independent experiments. ns, not significant, P>0.05; **P<0.01; one-way ANOVA with Tukey’s correction.
Anti Tomm20, supplied by Boster Bio, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tom20+tomm20/Anti-Tom20+TOMM20+Antibody/pmc13042334-83-36-38
Average 94 stars, based on 1 article reviews
anti tomm20 - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
anti tom20  (Boster Bio)
93
Boster Bio anti tom20
Fig. 1. Histamine evokes transfer of Ca2+ from the ER to mitochondria through IP3Rs. (A) Wide-field fluorescence images of HeLa cells expressing <t>TOM20–</t> GFP (green) and Mito-R-GECO1 (pseudo-coloured in magenta); the overlay shows colocalization in white. Images are typical of four independent experiments. Manders’ coefficients for the fraction of Mito-R-GECO1 colocalizing with TOM20–GFP=0.88±0.07 (mean±s.d.), n=4. (B) HeLa cells expressing Mito-R-GECO1 (mitochondrial Ca2+ indicator) and loaded with Calbryte 520 (cytosolic Ca2+ indicator) were stimulated with histamine (100 μM) in Ca2+-free HBS. Fluorescence from the Ca2+-saturated indicators (Fmax) was determined by addition of CGP 37157 (CGP, 50 μM) to inhibit the mitochondrial Na+/Ca2+ exchanger and then ionomycin (Iono, 10 μM) with 20 mM CaCl2. Results (F/Fmax) show fluorescence recorded from an entire cell. Results are typical of at least ten experiments. (C) Summary results show peak changes in fluorescence (ΔF/Fmax) evoked by histamine (100 μM) alone, or after pre-incubation with FCCP (5 μM, 10 min). Results show values for individual cells and mean±s.d. from ten (control) or 15 cells (FCCP) from three independent experiments. ns, not significant, P>0.05; **P<0.01; one-way ANOVA with Tukey’s correction. (D) Western blots showing the effect of non-silencing (NS) siRNA or siRNA directed at MCU in HeLa cells. Protein loadings (μg/lane) and molecular mass markers (kDa) are shown. Results are typical of three independent experiments. MCU siRNA reduced MCU expression to 22±7% of its expression in cells treated with NS siRNA (mean±s.e.m., n=3). (E,F) Effects of histamine (100 μM) in Ca2+-free HBS on [Ca2+]c (Calbryte 520) (E) or [Ca2+]m (Mito-R-GECO1) (F) in single HeLa cells treated with NS or MCU siRNA. Results, reported as F/Fmax, are typical of 15–16 cells from three independent experiments. (G) Summary results show effects of histamine in HeLa cells treated with NS or MCU siRNA. Results (individual values, mean±s.d.) are from 16 (NS) or 15 cells (MCU siRNA) from three independent experiments. ns, not significant, P>0.05; **P<0.01; one-way ANOVA with Tukey’s correction.
Anti Tom20, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tom20+tomm20/Human+TOMM20+Recombinant+Protein/pmc12777122-48-49-50
Average 93 stars, based on 1 article reviews
anti tom20 - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
tomm20 antibody (4f3)  (Bio-Techne corporation)
94
Bio-Techne corporation tomm20 antibody (4f3)
Fig. 1. Histamine evokes transfer of Ca2+ from the ER to mitochondria through IP3Rs. (A) Wide-field fluorescence images of HeLa cells expressing <t>TOM20–</t> GFP (green) and Mito-R-GECO1 (pseudo-coloured in magenta); the overlay shows colocalization in white. Images are typical of four independent experiments. Manders’ coefficients for the fraction of Mito-R-GECO1 colocalizing with TOM20–GFP=0.88±0.07 (mean±s.d.), n=4. (B) HeLa cells expressing Mito-R-GECO1 (mitochondrial Ca2+ indicator) and loaded with Calbryte 520 (cytosolic Ca2+ indicator) were stimulated with histamine (100 μM) in Ca2+-free HBS. Fluorescence from the Ca2+-saturated indicators (Fmax) was determined by addition of CGP 37157 (CGP, 50 μM) to inhibit the mitochondrial Na+/Ca2+ exchanger and then ionomycin (Iono, 10 μM) with 20 mM CaCl2. Results (F/Fmax) show fluorescence recorded from an entire cell. Results are typical of at least ten experiments. (C) Summary results show peak changes in fluorescence (ΔF/Fmax) evoked by histamine (100 μM) alone, or after pre-incubation with FCCP (5 μM, 10 min). Results show values for individual cells and mean±s.d. from ten (control) or 15 cells (FCCP) from three independent experiments. ns, not significant, P>0.05; **P<0.01; one-way ANOVA with Tukey’s correction. (D) Western blots showing the effect of non-silencing (NS) siRNA or siRNA directed at MCU in HeLa cells. Protein loadings (μg/lane) and molecular mass markers (kDa) are shown. Results are typical of three independent experiments. MCU siRNA reduced MCU expression to 22±7% of its expression in cells treated with NS siRNA (mean±s.e.m., n=3). (E,F) Effects of histamine (100 μM) in Ca2+-free HBS on [Ca2+]c (Calbryte 520) (E) or [Ca2+]m (Mito-R-GECO1) (F) in single HeLa cells treated with NS or MCU siRNA. Results, reported as F/Fmax, are typical of 15–16 cells from three independent experiments. (G) Summary results show effects of histamine in HeLa cells treated with NS or MCU siRNA. Results (individual values, mean±s.d.) are from 16 (NS) or 15 cells (MCU siRNA) from three independent experiments. ns, not significant, P>0.05; **P<0.01; one-way ANOVA with Tukey’s correction.
Tomm20 Antibody (4f3), supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tom20+tomm20/TOMM20+Antibody+(4F3)/bio-techne+corporation___h00009804-m01
Average 94 stars, based on 1 article reviews
tomm20 antibody (4f3) - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
anti-tomm20/tom20 antibody  (Boster Bio)
N/A
Boster Bio Anti-TOMM20/Tom20 Antibody (Catalog # A04039). Tested in WB applications. This antibody reacts with Human.
   Buy from Supplier
anti-tomm20/tom20 rabbit monoclonal antibody  (Boster Bio)
N/A
Boster Bio Anti-TOMM20/Tom20 Rabbit Monoclonal Antibody catalog # M04039. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
   Buy from Supplier

Image Search Results


Fig. 1. Histamine evokes transfer of Ca2+ from the ER to mitochondria through IP3Rs. (A) Wide-field fluorescence images of HeLa cells expressing TOM20– GFP (green) and Mito-R-GECO1 (pseudo-coloured in magenta); the overlay shows colocalization in white. Images are typical of four independent experiments. Manders’ coefficients for the fraction of Mito-R-GECO1 colocalizing with TOM20–GFP=0.88±0.07 (mean±s.d.), n=4. (B) HeLa cells expressing Mito-R-GECO1 (mitochondrial Ca2+ indicator) and loaded with Calbryte 520 (cytosolic Ca2+ indicator) were stimulated with histamine (100 μM) in Ca2+-free HBS. Fluorescence from the Ca2+-saturated indicators (Fmax) was determined by addition of CGP 37157 (CGP, 50 μM) to inhibit the mitochondrial Na+/Ca2+ exchanger and then ionomycin (Iono, 10 μM) with 20 mM CaCl2. Results (F/Fmax) show fluorescence recorded from an entire cell. Results are typical of at least ten experiments. (C) Summary results show peak changes in fluorescence (ΔF/Fmax) evoked by histamine (100 μM) alone, or after pre-incubation with FCCP (5 μM, 10 min). Results show values for individual cells and mean±s.d. from ten (control) or 15 cells (FCCP) from three independent experiments. ns, not significant, P>0.05; **P<0.01; one-way ANOVA with Tukey’s correction. (D) Western blots showing the effect of non-silencing (NS) siRNA or siRNA directed at MCU in HeLa cells. Protein loadings (μg/lane) and molecular mass markers (kDa) are shown. Results are typical of three independent experiments. MCU siRNA reduced MCU expression to 22±7% of its expression in cells treated with NS siRNA (mean±s.e.m., n=3). (E,F) Effects of histamine (100 μM) in Ca2+-free HBS on [Ca2+]c (Calbryte 520) (E) or [Ca2+]m (Mito-R-GECO1) (F) in single HeLa cells treated with NS or MCU siRNA. Results, reported as F/Fmax, are typical of 15–16 cells from three independent experiments. (G) Summary results show effects of histamine in HeLa cells treated with NS or MCU siRNA. Results (individual values, mean±s.d.) are from 16 (NS) or 15 cells (MCU siRNA) from three independent experiments. ns, not significant, P>0.05; **P<0.01; one-way ANOVA with Tukey’s correction.

Journal: Journal of cell science

Article Title: KRAP regulates mitochondrial Ca2+ uptake by licensing IP3 receptor activity and stabilizing ER-mitochondrial junctions.

doi: 10.1242/jcs.261728

Figure Lengend Snippet: Fig. 1. Histamine evokes transfer of Ca2+ from the ER to mitochondria through IP3Rs. (A) Wide-field fluorescence images of HeLa cells expressing TOM20– GFP (green) and Mito-R-GECO1 (pseudo-coloured in magenta); the overlay shows colocalization in white. Images are typical of four independent experiments. Manders’ coefficients for the fraction of Mito-R-GECO1 colocalizing with TOM20–GFP=0.88±0.07 (mean±s.d.), n=4. (B) HeLa cells expressing Mito-R-GECO1 (mitochondrial Ca2+ indicator) and loaded with Calbryte 520 (cytosolic Ca2+ indicator) were stimulated with histamine (100 μM) in Ca2+-free HBS. Fluorescence from the Ca2+-saturated indicators (Fmax) was determined by addition of CGP 37157 (CGP, 50 μM) to inhibit the mitochondrial Na+/Ca2+ exchanger and then ionomycin (Iono, 10 μM) with 20 mM CaCl2. Results (F/Fmax) show fluorescence recorded from an entire cell. Results are typical of at least ten experiments. (C) Summary results show peak changes in fluorescence (ΔF/Fmax) evoked by histamine (100 μM) alone, or after pre-incubation with FCCP (5 μM, 10 min). Results show values for individual cells and mean±s.d. from ten (control) or 15 cells (FCCP) from three independent experiments. ns, not significant, P>0.05; **P<0.01; one-way ANOVA with Tukey’s correction. (D) Western blots showing the effect of non-silencing (NS) siRNA or siRNA directed at MCU in HeLa cells. Protein loadings (μg/lane) and molecular mass markers (kDa) are shown. Results are typical of three independent experiments. MCU siRNA reduced MCU expression to 22±7% of its expression in cells treated with NS siRNA (mean±s.e.m., n=3). (E,F) Effects of histamine (100 μM) in Ca2+-free HBS on [Ca2+]c (Calbryte 520) (E) or [Ca2+]m (Mito-R-GECO1) (F) in single HeLa cells treated with NS or MCU siRNA. Results, reported as F/Fmax, are typical of 15–16 cells from three independent experiments. (G) Summary results show effects of histamine in HeLa cells treated with NS or MCU siRNA. Results (individual values, mean±s.d.) are from 16 (NS) or 15 cells (MCU siRNA) from three independent experiments. ns, not significant, P>0.05; **P<0.01; one-way ANOVA with Tukey’s correction.

Article Snippet: Plasmids encoding the following proteins were used: TOM20 [translocase of outer (mitochondrial) membrane 20, also known as TOMM20] tagged to GFP (TOM20–GFP) (Prole and Taylor, 2019a), Mito-R-GECO1 (Addgene, #46021) (Wu et al., 2013), human KRAP with an N-terminal myc-DDK tag (OriGene, Rockville, MD, USA, #RC205550) and siRNA-resistant human KRAP with an N-terminal myc-DDK tag (Thillaiappan et al., 2021).

Techniques: Fluorescence, Expressing, Incubation, Control, Western Blot