t7e1 nuclease Search Results


t7e1 nuclease assay  (New England Biolabs)
99
New England Biolabs t7e1 nuclease assay
T7e1 Nuclease Assay, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t7e1+nuclease/T7+Endonuclease+I/us11447774-1657-29-32
Average 99 stars, based on 1 article reviews
t7e1 nuclease assay - by Bioz Stars, 2026-10
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t7 endonuclease 1  (New England Biolabs)
99
New England Biolabs t7 endonuclease 1
Quantification of InDels produced by CRISPR-Cas9 activity at the TCRα locus indicates efficient cleavage. A . T7 <t>Endonuclease</t> <t>1</t> <t>(T7E1)</t> assay for three independent experiments. For each experiment, one lane for Non-Specific gRNA (N.S.) and one lane for TCRα-targeting gRNA treated cells are presented. Experiments are separated by a black bar. Percentages, presented below the lanes, refer to cleavage efficiency as inferred by densitometric analysis. Loading Ladder and relative sizes are indicated on the left. Unprocessed scan can be found in Supplementary Data 1. B . TIDE Analysis for the same experiments as in A. In the left panel, the height of each bar corresponds to the rate of sequences having the given number of nucleotides added or deleted. The right panel depicts the rate of sequence misalignments at each position of the PCR fragment amplified from the TCRα locus of cells treated with either the TCRα-targeting gRNA (green) or a Non-Specific gRNA (black) C . Quantification of A and B.
T7 Endonuclease 1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t7e1+nuclease/T7+Endonuclease+I/bio_rxiv__2021__08__20__457092-142-13-16
Average 99 stars, based on 1 article reviews
t7 endonuclease 1 - by Bioz Stars, 2026-10
99/100 stars
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t7e1 mismatch analysis  (New England Biolabs)
99
New England Biolabs t7e1 mismatch analysis
Quantification of InDels produced by CRISPR-Cas9 activity at the TCRα locus indicates efficient cleavage. A . T7 <t>Endonuclease</t> <t>1</t> <t>(T7E1)</t> assay for three independent experiments. For each experiment, one lane for Non-Specific gRNA (N.S.) and one lane for TCRα-targeting gRNA treated cells are presented. Experiments are separated by a black bar. Percentages, presented below the lanes, refer to cleavage efficiency as inferred by densitometric analysis. Loading Ladder and relative sizes are indicated on the left. Unprocessed scan can be found in Supplementary Data 1. B . TIDE Analysis for the same experiments as in A. In the left panel, the height of each bar corresponds to the rate of sequences having the given number of nucleotides added or deleted. The right panel depicts the rate of sequence misalignments at each position of the PCR fragment amplified from the TCRα locus of cells treated with either the TCRα-targeting gRNA (green) or a Non-Specific gRNA (black) C . Quantification of A and B.
T7e1 Mismatch Analysis, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t7e1+nuclease/Nuclease/pmc09197527-219-12-22
Average 99 stars, based on 1 article reviews
t7e1 mismatch analysis - by Bioz Stars, 2026-10
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nuclease t7e1  (Vazyme Biotech Co)
93
Vazyme Biotech Co nuclease t7e1
Quantification of InDels produced by CRISPR-Cas9 activity at the TCRα locus indicates efficient cleavage. A . T7 <t>Endonuclease</t> <t>1</t> <t>(T7E1)</t> assay for three independent experiments. For each experiment, one lane for Non-Specific gRNA (N.S.) and one lane for TCRα-targeting gRNA treated cells are presented. Experiments are separated by a black bar. Percentages, presented below the lanes, refer to cleavage efficiency as inferred by densitometric analysis. Loading Ladder and relative sizes are indicated on the left. Unprocessed scan can be found in Supplementary Data 1. B . TIDE Analysis for the same experiments as in A. In the left panel, the height of each bar corresponds to the rate of sequences having the given number of nucleotides added or deleted. The right panel depicts the rate of sequence misalignments at each position of the PCR fragment amplified from the TCRα locus of cells treated with either the TCRα-targeting gRNA (green) or a Non-Specific gRNA (black) C . Quantification of A and B.
Nuclease T7e1, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t7e1+nuclease/T7+Endonuclease+I/pm28528977-72-10-12
Average 93 stars, based on 1 article reviews
nuclease t7e1 - by Bioz Stars, 2026-10
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t7e1 nuclease  (New England Biolabs)
99
New England Biolabs t7e1 nuclease
Quantification of InDels produced by CRISPR-Cas9 activity at the TCRα locus indicates efficient cleavage. A . T7 <t>Endonuclease</t> <t>1</t> <t>(T7E1)</t> assay for three independent experiments. For each experiment, one lane for Non-Specific gRNA (N.S.) and one lane for TCRα-targeting gRNA treated cells are presented. Experiments are separated by a black bar. Percentages, presented below the lanes, refer to cleavage efficiency as inferred by densitometric analysis. Loading Ladder and relative sizes are indicated on the left. Unprocessed scan can be found in Supplementary Data 1. B . TIDE Analysis for the same experiments as in A. In the left panel, the height of each bar corresponds to the rate of sequences having the given number of nucleotides added or deleted. The right panel depicts the rate of sequence misalignments at each position of the PCR fragment amplified from the TCRα locus of cells treated with either the TCRα-targeting gRNA (green) or a Non-Specific gRNA (black) C . Quantification of A and B.
T7e1 Nuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t7e1+nuclease/Proteinase+K/10__1002_slash_adfm__202301256-400-2-7
Average 99 stars, based on 1 article reviews
t7e1 nuclease - by Bioz Stars, 2026-10
99/100 stars
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Image Search Results


Quantification of InDels produced by CRISPR-Cas9 activity at the TCRα locus indicates efficient cleavage. A . T7 Endonuclease 1 (T7E1) assay for three independent experiments. For each experiment, one lane for Non-Specific gRNA (N.S.) and one lane for TCRα-targeting gRNA treated cells are presented. Experiments are separated by a black bar. Percentages, presented below the lanes, refer to cleavage efficiency as inferred by densitometric analysis. Loading Ladder and relative sizes are indicated on the left. Unprocessed scan can be found in Supplementary Data 1. B . TIDE Analysis for the same experiments as in A. In the left panel, the height of each bar corresponds to the rate of sequences having the given number of nucleotides added or deleted. The right panel depicts the rate of sequence misalignments at each position of the PCR fragment amplified from the TCRα locus of cells treated with either the TCRα-targeting gRNA (green) or a Non-Specific gRNA (black) C . Quantification of A and B.

Journal: bioRxiv

Article Title: Frequent Aneuploidy in Primary Human T Cells Following CRISPR-Cas9 cleavage

doi: 10.1101/2021.08.20.457092

Figure Lengend Snippet: Quantification of InDels produced by CRISPR-Cas9 activity at the TCRα locus indicates efficient cleavage. A . T7 Endonuclease 1 (T7E1) assay for three independent experiments. For each experiment, one lane for Non-Specific gRNA (N.S.) and one lane for TCRα-targeting gRNA treated cells are presented. Experiments are separated by a black bar. Percentages, presented below the lanes, refer to cleavage efficiency as inferred by densitometric analysis. Loading Ladder and relative sizes are indicated on the left. Unprocessed scan can be found in Supplementary Data 1. B . TIDE Analysis for the same experiments as in A. In the left panel, the height of each bar corresponds to the rate of sequences having the given number of nucleotides added or deleted. The right panel depicts the rate of sequence misalignments at each position of the PCR fragment amplified from the TCRα locus of cells treated with either the TCRα-targeting gRNA (green) or a Non-Specific gRNA (black) C . Quantification of A and B.

Article Snippet: Resulting amplicons were denatured and reannealed in a thermocycler before nuclease reaction using T7 Endonuclease 1 (New England Biolabs) at 37C for 20min.

Techniques: Produced, CRISPR, Activity Assay, Sequencing, Amplification

Quantification of InDels produced by CRISPR-Cas9 activity at the TCRα, TCRβ and PDCD1 loci indicates efficient cleavage. A . T7E1 assay for the 4 genomic target loci. Non-Specific gRNA treated cells (4 left lanes) are compared to cells treated with the combination of the TCRα, TCRβ and PDCD1-targeting gRNAs (4 right lanes). In each lane, a different locus is analyzed. Calculated efficiency is indicated below. Loading Ladder and relative sizes are indicated in the middle. Unprocessed scan can be found in Supplementary Data 1. B . TIDE Analysis for the same experiments as in A. The rows represent (in this order, from top to bottom) the TCRα, TRBC1, TRBC2 and PDCD1 locus. In the left panels, the height of each bar corresponds to the rate of sequences having the given number of nucleotides added or deleted. The right panels depict the rates of sequence misalignments at each position of the PCR fragment amplified from the target locus of cells treated with either Non-Specific gRNA (black) or the combination of the TCRα, TCRβ and PDCD1-targeting gRNAs (green).

Journal: bioRxiv

Article Title: Frequent Aneuploidy in Primary Human T Cells Following CRISPR-Cas9 cleavage

doi: 10.1101/2021.08.20.457092

Figure Lengend Snippet: Quantification of InDels produced by CRISPR-Cas9 activity at the TCRα, TCRβ and PDCD1 loci indicates efficient cleavage. A . T7E1 assay for the 4 genomic target loci. Non-Specific gRNA treated cells (4 left lanes) are compared to cells treated with the combination of the TCRα, TCRβ and PDCD1-targeting gRNAs (4 right lanes). In each lane, a different locus is analyzed. Calculated efficiency is indicated below. Loading Ladder and relative sizes are indicated in the middle. Unprocessed scan can be found in Supplementary Data 1. B . TIDE Analysis for the same experiments as in A. The rows represent (in this order, from top to bottom) the TCRα, TRBC1, TRBC2 and PDCD1 locus. In the left panels, the height of each bar corresponds to the rate of sequences having the given number of nucleotides added or deleted. The right panels depict the rates of sequence misalignments at each position of the PCR fragment amplified from the target locus of cells treated with either Non-Specific gRNA (black) or the combination of the TCRα, TCRβ and PDCD1-targeting gRNAs (green).

Article Snippet: Resulting amplicons were denatured and reannealed in a thermocycler before nuclease reaction using T7 Endonuclease 1 (New England Biolabs) at 37C for 20min.

Techniques: Produced, CRISPR, Activity Assay, Sequencing, Amplification