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Image Search Results
Journal: Frontiers in Oncology
Article Title: Carnosol, a Natural Polyphenol, Inhibits Migration, Metastasis, and Tumor Growth of Breast Cancer via a ROS-Dependent Proteasome Degradation of STAT3
doi: 10.3389/fonc.2019.00743
Figure Lengend Snippet: Carnosol inhibits the STAT3 signaling pathway. (A) Concentration-dependent decrease of phospho-STAT3 and STAT3 protein in carnosol-treated MDA-MB-231 cells. Cells were treated with vehicle (DMSO) or indicated concentrations of carnosol for 24 h, then whole-cell extracts were subjected to Western blot analysis for the phosphorylated and non-phosphorylated form of STAT3 and for β-actin (loading control). (B) Carnosol decreases the level of STAT3 protein in Hs578T, MCF-7, and T47D breast cancer cell lines. Whole cell lysates from different breast cancer cell lines treated with the indicated concentrations of carnosol were resolved on 8% SDS-PAGE and analyzed by western blot for STAT3 protein. (C) Western blot analysis of STAT3 protein level in HCT116 colorectal cancer cells. Western blotting was performed as described above.
Article Snippet: Human breast cancer cells MDA-MB-231(cat# 300275), MCF-7 (cat# 300273) and
Techniques: Concentration Assay, Western Blot, Control, SDS Page