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Image Search Results
Journal: Cellular signalling
Article Title: Elucidating ferroptosis mechanisms in heart failure through transcriptomics, single-cell sequencing, and experimental validation.
doi: 10.1016/j.cellsig.2024.111416
Figure Lengend Snippet: Fig. 2. Screening to DFRGs from HF dataset. (A). Volcano map of GSE5406. (B). The heatmap of 127 DFRGs in GSE5406. (C). Correlation between the expression of 127 DFRGs. (D-E). The main GO terms and KEGG pathways enriched by differentially expressed mRNAs. (F). The performance in of ten-time cross-verification for tuning parameter in selection LASSO. Each coefficient curve in the upper picture represents a single gene. The solid vertical lines in another picture represent the partial likelihood deviance SE, and the number of genes (n = 10) corresponding to the lowest point of the curve is the most suitable for LASSO. (G). Accuracy graph and cross validation error graph of DFRGs screened with the SVM-RFE algorithm. (H). Venn diagram of the intersection of DFRGs (PTGS2, BECN1, SLC39A14, QSOX1, MLST8, TMSB4X and KDM4A) screened with the two machine learning methods.
Article Snippet: SLC39A14 (Cat No: 26540–1-AP) and
Techniques: Expressing, Selection, Biomarker Discovery
Journal: Cellular signalling
Article Title: Elucidating ferroptosis mechanisms in heart failure through transcriptomics, single-cell sequencing, and experimental validation.
doi: 10.1016/j.cellsig.2024.111416
Figure Lengend Snippet: Fig. 5. Expression of 7 key DFRGs in the single-cell sequencing dataset. (A). 10 cell types were identified, including B cell, Cardiomyocyte, Dendritic cell, Endothelial cell, Fibroblast, Macrophage, Mast cell, Neutrophils, NK cell, T cell. (B). Compared with normal tissue, cardiomyocytes, neutrophils, and B cells accounted for a decreased percentage of total cells and macrophages, endothelial cells dendritic cells, NK cells, and T cells accounted for an increased percentage of total cells in heart failure tissue. (C–D). Expression of 7 DFRGs in 10 cell types. MSB4X was mainly expressed in immune cells such as T cells, NK cells, and macrophages, MLST8 was mainly expressed in mast cells and fibroblasts, QSOX1 was mainly expressed in T cells, mast cells, and fibroblasts, and SLC39A14 was mainly expressed in neu trophils, mast cells, and cardiomyocytes. (E). BECN1, TMSB4X, and KDM4A had no altered expression in control and HF cell models, whereas the expression of MLST8, PTG32 and QSXO1 and SLC39A14 differed between the two groups.
Article Snippet: SLC39A14 (Cat No: 26540–1-AP) and
Techniques: Expressing, Sequencing, Control
Journal: Cellular signalling
Article Title: Elucidating ferroptosis mechanisms in heart failure through transcriptomics, single-cell sequencing, and experimental validation.
doi: 10.1016/j.cellsig.2024.111416
Figure Lengend Snippet: Fig. 6. Differential expression of QSXO1 and SLC39A14 was verified in the vivo. (A). Body weight levels of mice in each group during HFpEF modeling. (B). Changes in blood pressure in each group. (C). There were no statistically significant differences in left ventricular ejection fraction and left ventricular shortening fraction in the HFpEF group of mice compared to the control group. (D). The E/A ratio was significantly higher in the HFpEF group than in the control group. (E-F). MASSON staining of the HFpEF and control groups. (G). Western blot results showed that the protein expression of QSOX1 was higher than that of the control group, and the protein expression of SLC39A14 was lower than that of the control group. (H). The expression of QSOX1 was found to be higher in the HFpEF group than in the control group with the use of immunohistochemical staining. (I). The expression of SLC39A14 was found to be higher in the HFpEF group than in the control group with the use of immunohistochemical staining.
Article Snippet: SLC39A14 (Cat No: 26540–1-AP) and
Techniques: Quantitative Proteomics, Control, Staining, Western Blot, Expressing, Immunohistochemical staining