Journal: Journal of Nuclear Medicine

Article Title: Preclinical Evaluation of RYM1, a Matrix Metalloproteinase–Targeted Tracer for Imaging Aneurysm

doi: 10.2967/jnumed.116.188656

Figure Lengend Snippet: 99mTc-RYM1 imaging of AAA. (A and B) Examples of fused 99mTc-RYM1 SPECT/CT images of animals from the low remodeling (A) and aneurysm (B) groups, classified on the basis of visual in situ analysis of abdominal aorta. Transversal (left), coronal (middle), and sagittal (right) views are shown. Arrows point to areas of maximal tracer uptake in abdominal aorta. (C) Quantification of 99mTc-RYM1 signal in area of maximal tracer uptake in suprarenal abdominal aorta in low remodeling and AAA groups. *P < 0.05. (D) Correlation between 99mTc-RYM1 signal in vivo and MMP activity quantified by zymography ex vivo. AU = arbitrary units; cpv = counts per voxel; LR = low remodeling.

Article Snippet: RYM1 inhibition constants (K i ) for recombinant human MMP-2, -7, -9, -12, and -13 (R&D Systems) were evaluated in vitro using a fluorogenic MMP activity assay based on Mca-KPLGL-Dpa-AR-NH2 fluorogenic peptide substrate (R&D Systems), as previously described with minor modifications ( 8 ).

Techniques: Imaging, Single Photon Emission Computed Tomography, In Situ, In Vivo, Activity Assay, Zymography, Ex Vivo

Journal: Journal of Nuclear Medicine

Article Title: Preclinical Evaluation of RYM1, a Matrix Metalloproteinase–Targeted Tracer for Imaging Aneurysm

doi: 10.2967/jnumed.116.188656

Figure Lengend Snippet: Ex vivo characterization of suprarenal abdominal aorta. (A) Maximal external diameter of abdominal aorta in low remodeling and AAA groups. (B) Aortic MMP activity quantified by zymography in low remodeling and AAA groups. **P < 0.01. AU = arbitrary units; LR = low remodeling.

Article Snippet: RYM1 inhibition constants (K i ) for recombinant human MMP-2, -7, -9, -12, and -13 (R&D Systems) were evaluated in vitro using a fluorogenic MMP activity assay based on Mca-KPLGL-Dpa-AR-NH2 fluorogenic peptide substrate (R&D Systems), as previously described with minor modifications ( 8 ).

Techniques: Ex Vivo, Activity Assay, Zymography

Journal: Cell

Article Title: Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis

doi: 10.1016/j.cell.2018.05.028

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Assay was performed in triplicates in a reaction buffer (50 mM sodium acetate, 8 mM EDTA, 1 mM DTT, 1 μM pefabloc, all Sigma-Aldrich) using 2 μg of protein lysate and 10 μM protease substrate z-LR-AMC (ES008, R&D Systems).

Techniques: In Vivo, Flow Cytometry, Recombinant, Staining, Cell Isolation, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Glucose Assay, Reverse Transcription, SYBR Green Assay, Control, Software