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All (1)
STEMCELL Technologies
 

About 11 products / 111 snippets / 4 images

91 / 100
Bioz Stars
StemSpan ACF
STEMCELL Technologies  
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Techniques
 
Article Snippets
".. taking care to minimize the removal any floating cell clusters, and replaced with StemSpan ACF (StemCell Technologies Inc.) supplemented with recombinant human (rh) BMP-4 at 50 ng/ml, rh VEGF at 50 .."  (see more)
90 / 100
Bioz Stars
StemSpan ACF
STEMCELL Technologies  
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Techniques
 
Article Snippets
"To make 20ml Erythroblast Expansion Medium, combine: 18.8 ml StemSpan ACF (Stemcell Technologies, cat. no. 09855) 1.2 ml Autologous Patient Serum (6% final), see SUPPORT PROTOCOL 1 .."  (see more)
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Expansion and maturation of hiPSC-derived megakaryocytes (MKs) in different media conditions. ( A ) Experimental outline using the improved induction conditions. Megakaryocyte progenitors (MKPs) derived from stage II at days 9, 12, and 14 (purple arrows) were harvested and matured into MKs in stage III for up to 12 additional days in three different media <t>(StemSpan,</t> IMDM or APEL), supplemented as indicated. From day 9 + 10 on, MKs were cultured under dynamic conditions at 60 rpm. Cells were harvested, counted, and analyzed by flow cytometry (platelet counts are shown in B,C). ( B ) The total amount of CD61 + /CD41a + /CD42b + maturing MKs revealed no significant difference after culture in the three different media (StemSpan, down pointing triangle; IMDM, squares; APEL, upward pointing triangles; three different mean value lines representing the three media; symbol color depicts the two independent hiPSC clones UCB144-CT2-C, grey and BIHi001-A, black; n = 4, p > 0.05; two-way ANOVA, multiple comparisons). ( C ) MKs showed various maturation stages, indicated by two or more nuclei (deep purple), independent of media conditions (May–Grünwald Giemsa stain, MGG; top row, lower magnification inserts). Characteristic MK immunophenotype CD42b (green), CD61 (red); nuclear DAPI stain (blue; lower row). Representative pictures of two replicates of clone BIHi001-A are shown after harvest on day 9 + 7 (stage III) and stained as specified.
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Human iPSC-derived megakaryocytes (MKs) produce pro-platelets and release platelets in all media conditions. ( A ) MKs with pro-platelet structures on day 9 + 12 (stage III) after dynamic culture in supplemented <t>StemSpan,</t> IMDM, and APEL media. Representative immunofluorescence images of MKs from one hiPSC clone (UCB144-CT2-C) stained with CD42b (green), CD61 (red), and DAPI (nuclei, blue) are shown. Pro-platelets are indicated by white arrows. ( B ) Platelet counts on day 9 + 12 (stage III) calculated per input MK were significantly increased after culture in supplemented IMDM medium, compared to StemSpan ( p = 0.046) and APEL medium ( p = 0.0273; both unpaired t-tests * p < 0.05 and ** p < 0.01). ( C ) Additional analysis showed comparable platelet counts per 1 × 10 6 input hiPSCs ( p > 0.05) in all media conditions. Results from two independent hiPSC clones (UCB144-CT2-C, grey circles, and BIHi001-A, black diamonds; n = 8, measured in duplicates).
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Media conditions.
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85 / 100
Bioz Stars
StemSpan ™ -ACF
STEMCELL Technologies  
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Techniques
 
Article Snippets
"Cryopreserved human umbilical Cord Blood (hUCB) CD34 + cells (Lonza, 2C-101) were thawed and expanded in StemSpan TM -ACF (STEMCELLTM Technologies, 09805) supplemented with 100 ng/mL recombinant human (rh)SCF (PeproTech, AF-300-07), 100 ng/ml rhFlt3-ligand (PeproTech, .."  (see more)
80 / 100
Bioz Stars
StemSpan-ACF medium
STEMCELL Technologies  
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Techniques
 
Article Snippets
"In brief, the PBMCs were cultured for 5 days in MNC medium; StemSpan-ACF medium (STEMCELL Technologies, Vancouver, Canada), supplemented with 100 ng/ml rhSCF, 100 ng/ml rhTPO, 100 ng/ml rhFlt3L, 50 .."  (see more)
91 / 100
Bioz Stars
StemSpan-ACF medium
STEMCELL Technologies  
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Techniques
 
Article Snippets
".. a density gradient separation procedure and further enriched for erythroblasts with StemSpan-ACF medium (09860; STEMCELL Technologies, Vancouver, Canada) supplemented with StemSpan Erythroid Expansion Supplement 100£ (02692; STEMCELL .."  (see more)
80 / 100
Bioz Stars
StemSpan ACF STEMCELL Technologies
STEMCELL Technologies  
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Techniques
 
Article Snippets
".. (MLN4924) Adooq Bioscience Cat#A11260-5 CSN inhibitor (1, 10-o-phenanthroline) Sigma Cat# 131377 StemSpan ACF STEMCELL Technologies Cat#09855 MEM a, nucleosides GIBCO Cat#12571-063 OptiMEM GIBCO Cat#31985070 Glutamax Invitrogen .."  (see more)
80 / 100
Bioz Stars
Stemspan Animal Component-Free (ACF) media
STEMCELL Technologies  
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Techniques
 
Article Snippets
"The pooled CD34 cells were cultured in serum-free Stemspan Animal Component-Free (ACF) media (Stem Cell Technologies) supplemented with 1% antibiotic/antimycotic (Life Technologies) and standard growth factors .."  (see more)
80 / 100
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StemSpan-XF: MesenCult-ACF Plus Medium
STEMCELL Technologies  
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Techniques
 
Article Snippets
"The cells were cultured in a 1:1 mixture of StemSpan-XF: MesenCult-ACF Plus Medium (Stem Cell Technologies)."  (see more)
80 / 100
Bioz Stars
StemSpan TM animal component free (ACF) medium
STEMCELL Technologies  
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Techniques
 
Article Snippets
"Where indicated, final maturation was performed with StemSpan TM animal component free (ACF) medium (Stemcell Technologies) with 5 IU/mL heparin (Merck Millipore) and 1× StemSpanTM .."  (see more)
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86 / 100
Bioz Stars
StemSpan-ACF StemCell Technology 9855 RH SCF Shenandoah Biotechnology
STEMCELL Technologies  
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Techniques
 
Article Snippets
".. MG-132 Adooq Bioscience A11043 JetPrime transfection reagent PolyPlus Transfection 114-15 StemSpan-ACF StemCell Technology 9855 RH SCF Shenandoah Biotechnology 100-04 RH FLT3L Shenandoah Biotechnology 100-21 Hu-TPO R&D system 288-TPN .."  (see more)
80 / 100
Bioz Stars
StemSpan-ACF-E
STEMCELL Technologies  
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Techniques
 
Article Snippets
"Abstract: As humans’ closest living relatives, chimpanzees offer valuable insights into human evolution.. However, technical and ethical limitations hinder investigations into the molecular and cellular foundations that distinguish chimpanzee ..."  (see more)