Journal: Journal of Bacteriology

Article Title: Sulfide Consumption in Sulfurimonas denitrificans and Heterologous Expression of Its Three Sulfide-Quinone Reductase Homologs

doi: 10.1128/JB.01021-15

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: Escherichia coli strains were cultured in lysogeny broth (LB) at 37°C. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Relevant feature(s) Reference or source E. coli DH5α Host strain for plasmid constructions, fhuA2 Δ (argF-lacZ)U169 phoA glnV44 φ80d lacZ ΔM15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 New England Biolabs S17-1 Donor strain for conjugation ( recA pro hsdR RP4-2-Tc::Mu-Km::Tn 7 ) 34 R. capsulatus SB1003 Wild type 52 SB1003 Δ sqr SB1003 Δ sqr ::Gm r This study S. denitrificans DSM-1251 Wild type 21 Plasmids pNPTS138-R6KT a Suicide plasmid for in-frame deletions, mobRP4 + ori -R6K sacB Km r 32 pNPTS138-R6KT_ΔsqrRC sqr (RCAP_rcc00785) deletion fragment (partially replaced with gentamicin resistance cassette) cloned into PstI-SalI sites of pNPTS138-R6KT, Gm r Km r This study pRK415 Cloning vector, can replicate in Rhodobacter , lacZ α IncP Tc r 36 pRK415_sqrRC sqr (RCAP_rcc00785) cloned into XbaI-HindIII sites of pRK415, Tc r This study pRK415_Suden619 Suden_619 cloned into EcoRI-HindIII sites of pRK415, Tc r This study pRK415_Suden1879 Suden_1879 cloned into BamHI-XbaI sites of pRK415, Tc r This study pRK415_Suden2082 Suden_2082 cloned into BamHI-XbaI sites of pRK415, Tc r This study pRK2013 Helper plasmid for the triparental conjugation, Km r 37 pBBR1MCS-5 Broad-host-range cloning vector, Gm r 31 pGEM-T Cloning vector with T overhangs, lacZ Ap r Promega Open in a separate window a The suicide vector pNTPS138-R6KT carries a sacB gene for a levansucrase from Bacillus subtilis that, when expressed, is lethal to Gram-negative bacteria in the presence of sucrose.

Techniques: Plasmid Preparation, Conjugation Assay, Clone Assay, Cloning

Journal:

Article Title: Functional Analysis by Site-Directed Mutagenesis of the NAD + -Reducing Hydrogenase from Ralstonia eutropha

doi: 10.1128/JB.184.22.6280-6288.2002

Figure Lengend Snippet: Strains and plasmids

Article Snippet: Antibiotics were added to a final concentration of 20 μg/ml (tetracycline) or 100 μg/ml (ampicillin). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Relevant characteristics a Source or reference Strains R. eutropha H16 SH + MBH + DSM 428, ATCC 17699 HF424 SH − MBH − 21 E. coli S17-1 recA pro thi hsdR chR ::RP4-2 Tra + 34 Plasmids pBluescript KS(+) Ap r lacZ ′ T7 φ10 promoter f1 ori Stratagene, cloning system pCH472 1.9-kb Sst I fragment of pGE15 in pBluescript KS(+) 22 pGE15 15.0-kb Hin dIII fragment of pHG1 in pVK101 37 pGE331 Derivative of pGE15 with a 1.9-kb Sst I fragment deleted 22 Open in a separate window a MBH, membrane-bound hydrogenase.

Techniques: Plasmid Preparation, Cloning