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RayBiotech

About 32 products / 96 snippets / 17 images

	80 / 100 Bioz Stars		RTK Phosphorylation Array RayBiotech		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets "The RTK Phosphorylation Array was purchased from Ray Biotech (AAH-PRTK-G1)." (see more)	× MORE IMAGES FROM THIS ARTICLE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query RTK Phosphorylation Array RayBiotech Article Snippet "" Figure Legend " " MORE IMAGES FROM THIS ARTICLE

	80 / 100 Bioz Stars		RTK phosphorylation array 1 RayBiotech		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets "Antibody arrays used in this study were as follows: RTK phosphorylation array 1 (cat. number 126AAH-PRTK-1-8, Raybiotech), Proteome Profiler Human Phospho-RTK and Phospho-Kinase Array Kit (ARY001B and .." (see more)	× MORE IMAGES FROM THIS ARTICLE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query RTK phosphorylation array 1 RayBiotech Article Snippet "" Figure Legend " " MORE IMAGES FROM THIS ARTICLE

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A Changes in protein kinase <t>phosphorylation</t> levels induced by C1GALT1 knockdown as detected using a Human Phospho-Kinase Array Kit. The chemiluminescent signal was detected using the ImageQuant LAS 4000 mini-imager (GE Healthcare). PC, positive control. B Relative signal levels of array spots in Fig. 5A. There were two blots corresponding to each protein for one membrane set, and the experiment was repeated twice for each membrane. Comparisons between conditions were determined using a t-test. Statistical significance is indicated as follows: * p < 0.05, ** p < 0.01, *** p < 0.001. NC negative control, KD knockdown. C Western blot confirming the effects of C1GALT1 knockdown on protein kinase expression. D Changes in receptor tyrosine kinase phosphorylation levels induced by C1GALT1 knockdown as detected using the Human <t>Phospho-RTK</t> Array Kit. E Relative signal levels of array spots in Fig. 5D. F Western blot confirming the effects of C1GALT1 knockdown on receptor tyrosine kinases.

Phospho-proteomics

KD

POS-CON

MEM

NEGA-C

(A) Relative change in <t>RTK</t> <t>phosphorylation</t> in OR1-7 H1975 populations normalized to parental H1975 controls from RTK phosphorylation arrays in Fig. S2. Red numbers indicate increased tyrosine phosphorylation of the indicated kinase over parental controls. Colored arrows indicate drug targets for inhibitors (B) tested in combination with osimertinib in (C), large arrows indicate the primary drug target whereas small arrows indicate the multiple targets for a given color coded inhibitor. * Indicates EPH receptor family members with increased phosphorylation across resistant clones. (B) Inhibitors to common osimertinib resistance mechanisms observed in A; bold indicated the primary drug target for each inhibitor. (C) Synergy scores showing the relative abilities of osimertinib to enhance the efficacy of secondary inhbitors [Log(α 1 )] or these inhibitors enhance the efficacy [Log(α 2 )] or observed potency (β obs ) of osimertinib in OR1-7 H1975 populations. (D) Heat maps of cell viability (top) and excess over Bliss (EOB, bottom) for OR1-7 H1975 populations treated with increasing (semilog) doses of RMC-4550 (10 −8 – 10 −5.5 ), osimertinib (10 −10.5 – 10 −6 ), or the combination of RMC-4550+osimertnib over 6×10 dose matrix. Sum EOB indicates the extent of synergy across the dose matrix. (E) Synergy scores showing the relative changes in efficacy or observed potency of in OR1-7 H1975 populations treated with osimertinib + RMC-4550 from (D).

Phospho-proteomics

CLON-A

Osimertinib-resistant populations show hyperactivation of multiple RTKs and are sensitive to SHP2 inhibition (A) Quantitation of tyrosine <t>phosphorylation</t> of the indicated RTKs in whole-cell lysates from osimertinib-resistant H1975 populations OR1-8 relative to parental controls; samples were assessed for <t>RTK</t> phosphorylation using a human RTK phosphorylation antibody array (RayBiotech, complete array data in ). Data are mean ± SD from n = 3 replicate wells of the RTK array. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 vs. parental H1975 cells. (B–D) Quantitation of the change in % osimertinib efficacy (B, β obs ), fold change in osimertinib potency (C, log α 2 ), and a plot showing the change in osimertinib potency (log α 2 ) versus efficacy (β obs ) (D) from dose-response experiments assessing combined treatment of parental H1975 cells or OR1-8 populations with osimertinib +/− the indicated second RTK or RTK/RAS pathway inhibitor from . For (D), cells in the upper right quadrant (positive log α 2 and positive β obs ) represent the most promising osimertinib combination for a given cell population. Data in (B) and (C) are shown as the mean and 95% confidence interval, and data in (D) are the mean from n = 3 independent experiments. (E–G) Quantitation of the change in % osimertinib efficacy (E, β obs ), fold change in osimertinib potency (F, log α 2 ), and a plot showing the change in osimertinib potency (log α 2 ) versus efficacy (β obs ) (G) from dose-response experiments assessing combined treatment of parental H1975 cells or OR1-8 populations with osimertinib +/− the SHP2 inhibitor RMC-4550. Data in E and F are shown as the mean and 95% confidence interval, and data in G are the mean from n = 3 independent experiments. (H) Parental and osimertinib-resistant H1975 populations were treated with a matrix of doses of osimertinib +/− the SHP2 inhibitor RMC-4550 for four days, and cell viability was assessed using CellTitre glo. Excess over Bliss was calculated for each dose combination as a measure of synergy. Data are the mean from n = 3 independent experiments. (I) Schematic showing hyperactivation of multiple RTKs driving acquired osimertinib resistance. Inhibition of proximal RTK signaling using a SHP2 inhibitor, but not individual RTK inhibitors, synergizes with osimertinib by enhancing osimertinib efficacy and potency. (J) Western blots for pERK, ERK, HSP90, and tubulin from whole-cell lysates of parental and osimertinib-resistant H1975 populations (OR2, OR3, OR5, and OR6) left untreated or treated with 300 nM or 1000 nM osimertinib ±300 nM RMC-4550. Western blots are representative of n = 2 independent experiments.

INHIB

QUANT-A

Phospho-proteomics

AbA

WB

A PTC cell lines treated overnight with PLX4720 (1000 nM) were lysed and analyzed for <t>RTK</t> <t>phosphorylation</t> using the RayBio® C-Series Human Receptor Tyrosine Kinase Phosphorylation Antibody Array. B Composite intensity of staining across all 71 antibody targets in RayBio phosphorylation array to assess RTK signaling in MDA-T32 and MDA-T41 cells after 24-h incubation with DMSO or PLX4720 (1000 nM) were normalized to the intrinsic positive controls and graphed as a fold signal intensity. Error bars are ±SEM, n = 2. (RTK array antibody legend available in supplemental Fig. ). C Hallmark pathways enriched in MDA-T32 (black) and MDA-T41 (gray) bars. Data was obtained from for use with Gene Set Enrichment Analysis (GSEA) application for comparative analysis. Pathways with NES > 1.5, FDR < 0.05 are shown. D , E Volcano plot of Cancer Inflammatory and Immunity Crosstalk RT 2 qPCR array comparing gene expression in MDA-T32 cells to that of MDA-T41 after 24-h treatment with DMSO ( D ) or PLX4720 (1000 nM) ( E ). Orange dots denote select differentially expressed genes in inflammatory signaling and blue dots, mitogenic signaling.

Phospho-proteomics

AbA

STAIN

INCU

GENE-E

	80 / 100 Bioz Stars		Human RTK phosphorylation array RayBiotech		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets ".. as input (200–500 μg) for the Proteome Profiler Human Phospho-Kinase Array or Human RTK phosphorylation array (RayBiotech)." (see more)	Phospho-proteomics KD POS-CON MEM NEGA-C × MORE IMAGES FROM THIS ARTICLE Inhibition of the galactosyltransferase C1GALT1 reduces osteosarcoma cell proliferation by interfering with ERK signaling and cell cycle progression Cancer Gene Therapy Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Inhibition of the galactosyltransferase C1GALT1 reduces osteosarcoma cell proliferation by interfering with ERK signaling and cell cycle progression Cancer Gene Therapy 2000 Jan 01 Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Human RTK phosphorylation array RayBiotech Article Snippet "After 48 hours, cellular proteins were extracted using RIPA lysis buffer and used as input (200–500 μg) for the Proteome Profiler Human Phospho-Kinase Array or Human RTK phosphorylation array (RayBiotech)." Figure Legend "Fig. 5 A Changes in protein kinase phosphorylation levels induced by C1GALT1 knockdown as detected using a Human Phospho-Kinase Array Kit. The chemiluminescent signal was detected using the ImageQuant LAS 4000 mini-imager (GE Healthcare). PC, positive control. B Relative signal levels of array spots in Fig. 5A. There were two blots corresponding to each protein for one membrane set, and the experiment was repeated twice for each membrane. Comparisons between conditions were determined using a t-test. Statistical significance is indicated as follows: * p < 0.05, p < 0.01, * p < 0.001. NC negative control, KD knockdown. C Western blot confirming the effects of C1GALT1 knockdown on protein kinase expression. D Changes in receptor tyrosine kinase phosphorylation levels induced by C1GALT1 knockdown as detected using the Human Phospho-RTK Array Kit. E Relative signal levels of array spots in Fig. 5D. F Western blot confirming the effects of C1GALT1 knockdown on receptor tyrosine kinases. " Phospho-proteomics KD POS-CON MEM NEGA-C MORE IMAGES FROM THIS ARTICLE Cancer Gene Therapy Inhibition of the galactosyltransferase C1GALT1 reduces osteosarcoma cell proliferation by interfering with ERK signaling and cell cycle progression

	80 / 100 Bioz Stars		Mouse RTK Phosphorylation Array C1 RayBiotech		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets "Receptor phosphorylation was quantified using the Mouse RTK Phosphorylation Array C1 (RayBiotech) according to manufacturer’s instructions." (see more)	× MORE IMAGES FROM THIS ARTICLE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Mouse RTK Phosphorylation Array C1 RayBiotech Article Snippet "" Figure Legend " " MORE IMAGES FROM THIS ARTICLE

	80 / 100 Bioz Stars		Human RTK Phosphorylation Array C1 RayBiotech		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets "Human RTK Phosphorylation Array C1 , RayBiotech Life, Inc., Norcross, GA, USA , AAH-PRTK-1-2." (see more)	× MORE IMAGES FROM THIS ARTICLE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Human RTK Phosphorylation Array C1 RayBiotech Article Snippet "" Figure Legend " " MORE IMAGES FROM THIS ARTICLE

	80 / 100 Bioz Stars		Human RTK Phosphorylation Array C1 RayBiotech		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets "For tyrosine kinase protein array, Human RTK Phosphorylation Array C1 (AAH-PRTK-1-4, RayBiotech, Peachtree Corners, GA, USA) was used following the manufacturer’s instructions .." (see more)	× MORE IMAGES FROM THIS ARTICLE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Human RTK Phosphorylation Array C1 RayBiotech Article Snippet "" Figure Legend " " MORE IMAGES FROM THIS ARTICLE

	80 / 100 Bioz Stars		Human RTK Phosphorylation Array G1 RayBiotech		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets ".. RIPA buffer prior to assessing for RTK phosphorylation using a Human RTK Phosphorylation Array G1 (RayBiotech, AAH-PRTK-G1-8) per manufacturer’s instructions." (see more)	Phospho-proteomics CLON-A × MORE IMAGES FROM THIS ARTICLE In situ modeling of acquired resistance to RTK/RAS pathway targeted therapies bioRxiv Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us In situ modeling of acquired resistance to RTK/RAS pathway targeted therapies bioRxiv 2000 Jan 01 Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Human RTK Phosphorylation Array G1 RayBiotech Article Snippet "Clarified lysates were diluted to 1 mg/mL in RIPA buffer prior to assessing for RTK phosphorylation using a Human RTK Phosphorylation Array G1 (RayBiotech, AAH-PRTK-G1-8) per manufacturer’s instructions." Figure Legend "Fig 2. (A) Relative change in RTK phosphorylation in OR1-7 H1975 populations normalized to parental H1975 controls from RTK phosphorylation arrays in Fig. S2. Red numbers indicate increased tyrosine phosphorylation of the indicated kinase over parental controls. Colored arrows indicate drug targets for inhibitors (B) tested in combination with osimertinib in (C), large arrows indicate the primary drug target whereas small arrows indicate the multiple targets for a given color coded inhibitor. * Indicates EPH receptor family members with increased phosphorylation across resistant clones. (B) Inhibitors to common osimertinib resistance mechanisms observed in A; bold indicated the primary drug target for each inhibitor. (C) Synergy scores showing the relative abilities of osimertinib to enhance the efficacy of secondary inhbitors [Log(α 1 )] or these inhibitors enhance the efficacy [Log(α 2 )] or observed potency (β obs ) of osimertinib in OR1-7 H1975 populations. (D) Heat maps of cell viability (top) and excess over Bliss (EOB, bottom) for OR1-7 H1975 populations treated with increasing (semilog) doses of RMC-4550 (10 −8 – 10 −5.5 ), osimertinib (10 −10.5 – 10 −6 ), or the combination of RMC-4550+osimertnib over 6×10 dose matrix. Sum EOB indicates the extent of synergy across the dose matrix. (E) Synergy scores showing the relative changes in efficacy or observed potency of in OR1-7 H1975 populations treated with osimertinib + RMC-4550 from (D). " Phospho-proteomics CLON-A MORE IMAGES FROM THIS ARTICLE bioRxiv In situ modeling of acquired resistance to RTK/RAS pathway targeted therapies

	80 / 100 Bioz Stars		Human RTK Phosphorylation Array G1 RayBiotech		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets "Human RTK Phosphorylation Array G1 , RayBiotech , AAH-PRTK-G1-8." (see more)	INHIB QUANT-A Phospho-proteomics AbA WB × MORE IMAGES FROM THIS ARTICLE In situ modeling of acquired resistance to RTK/RAS-pathway-targeted therapies iScience Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us In situ modeling of acquired resistance to RTK/RAS-pathway-targeted therapies iScience 2000 Jan 01 Product Images (2) Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Human RTK Phosphorylation Array G1 RayBiotech Article Snippet "Human RTK Phosphorylation Array G1 , RayBiotech , AAH-PRTK-G1-8." Figure Legend "Figure 4 Osimertinib-resistant populations show hyperactivation of multiple RTKs and are sensitive to SHP2 inhibition (A) Quantitation of tyrosine phosphorylation of the indicated RTKs in whole-cell lysates from osimertinib-resistant H1975 populations OR1-8 relative to parental controls; samples were assessed for RTK phosphorylation using a human RTK phosphorylation antibody array (RayBiotech, complete array data in ). Data are mean ± SD from n = 3 replicate wells of the RTK array. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001 vs. parental H1975 cells. (B–D) Quantitation of the change in % osimertinib efficacy (B, β obs ), fold change in osimertinib potency (C, log α 2 ), and a plot showing the change in osimertinib potency (log α 2 ) versus efficacy (β obs ) (D) from dose-response experiments assessing combined treatment of parental H1975 cells or OR1-8 populations with osimertinib +/− the indicated second RTK or RTK/RAS pathway inhibitor from . For (D), cells in the upper right quadrant (positive log α 2 and positive β obs ) represent the most promising osimertinib combination for a given cell population. Data in (B) and (C) are shown as the mean and 95% confidence interval, and data in (D) are the mean from n = 3 independent experiments. (E–G) Quantitation of the change in % osimertinib efficacy (E, β obs ), fold change in osimertinib potency (F, log α 2 ), and a plot showing the change in osimertinib potency (log α 2 ) versus efficacy (β obs ) (G) from dose-response experiments assessing combined treatment of parental H1975 cells or OR1-8 populations with osimertinib +/− the SHP2 inhibitor RMC-4550. Data in E and F are shown as the mean and 95% confidence interval, and data in G are the mean from n = 3 independent experiments. (H) Parental and osimertinib-resistant H1975 populations were treated with a matrix of doses of osimertinib +/− the SHP2 inhibitor RMC-4550 for four days, and cell viability was assessed using CellTitre glo. Excess over Bliss was calculated for each dose combination as a measure of synergy. Data are the mean from n = 3 independent experiments. (I) Schematic showing hyperactivation of multiple RTKs driving acquired osimertinib resistance. Inhibition of proximal RTK signaling using a SHP2 inhibitor, but not individual RTK inhibitors, synergizes with osimertinib by enhancing osimertinib efficacy and potency. (J) Western blots for pERK, ERK, HSP90, and tubulin from whole-cell lysates of parental and osimertinib-resistant H1975 populations (OR2, OR3, OR5, and OR6) left untreated or treated with 300 nM or 1000 nM osimertinib ±300 nM RMC-4550. Western blots are representative of n = 2 independent experiments. " INHIB QUANT-A Phospho-proteomics AbA WB MORE IMAGES FROM THIS ARTICLE iScience In situ modeling of acquired resistance to RTK/RAS-pathway-targeted therapies

	86 / 100 Bioz Stars		Human RTK phosphorylation antibody array RayBiotech		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets "The RayBio® Human RTK phosphorylation antibody array kit (RayBiotech Inc., Norcross, GA, USA) was used for the Human RTK phosphorylation antibody array." (see more)	× MORE IMAGES FROM THIS ARTICLE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Human RTK phosphorylation antibody array RayBiotech Article Snippet "" Figure Legend " " MORE IMAGES FROM THIS ARTICLE

	80 / 100 Bioz Stars		Human RTK Phosphorylation Antibody Arrays RayBiotech		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets ".. IC50 (3 µM) for 72 h. The array was conducted using Human RTK Phosphorylation Antibody Arrays (Ray Biotech, Peachtree Corners, GA, USA) according to the manufacturer’s protocol." (see more)	× MORE IMAGES FROM THIS ARTICLE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Human RTK Phosphorylation Antibody Arrays RayBiotech Article Snippet "" Figure Legend " " MORE IMAGES FROM THIS ARTICLE

	80 / 100 Bioz Stars		C-series RTK Phosphorylation array C1 RayBiotech		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets "RayBioTech C-series RTK Phosphorylation array C1 (AAH-PRTK-1-8) was used to assess the active phosphorylation of 71 targets across different thyroid cancer cell lines." (see more)	Phospho-proteomics AbA STAIN INCU GENE-E × MORE IMAGES FROM THIS ARTICLE Mcl-1 mediates intrinsic resistance to RAF inhibitors in mutant BRAF papillary thyroid carcinoma Cell Death Discovery Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Mcl-1 mediates intrinsic resistance to RAF inhibitors in mutant BRAF papillary thyroid carcinoma Cell Death Discovery 2000 Jan 01 Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query C-series RTK Phosphorylation array C1 RayBiotech Article Snippet "RayBioTech C-series RTK Phosphorylation array C1 (AAH-PRTK-1-8) was used to assess the active phosphorylation of 71 targets across different thyroid cancer cell lines." Figure Legend "Fig. 2 A PTC cell lines treated overnight with PLX4720 (1000 nM) were lysed and analyzed for RTK phosphorylation using the RayBio® C-Series Human Receptor Tyrosine Kinase Phosphorylation Antibody Array. B Composite intensity of staining across all 71 antibody targets in RayBio phosphorylation array to assess RTK signaling in MDA-T32 and MDA-T41 cells after 24-h incubation with DMSO or PLX4720 (1000 nM) were normalized to the intrinsic positive controls and graphed as a fold signal intensity. Error bars are ±SEM, n = 2. (RTK array antibody legend available in supplemental Fig. ). C Hallmark pathways enriched in MDA-T32 (black) and MDA-T41 (gray) bars. Data was obtained from for use with Gene Set Enrichment Analysis (GSEA) application for comparative analysis. Pathways with NES > 1.5, FDR < 0.05 are shown. D , E Volcano plot of Cancer Inflammatory and Immunity Crosstalk RT 2 qPCR array comparing gene expression in MDA-T32 cells to that of MDA-T41 after 24-h treatment with DMSO ( D ) or PLX4720 (1000 nM) ( E ). Orange dots denote select differentially expressed genes in inflammatory signaling and blue dots, mitogenic signaling. " Phospho-proteomics AbA STAIN INCU GENE-E MORE IMAGES FROM THIS ARTICLE Cell Death Discovery Mcl-1 mediates intrinsic resistance to RAF inhibitors in mutant BRAF papillary thyroid carcinoma

	80 / 100 Bioz Stars		Human RTK Phosphorylation Array C1 kit RayBiotech		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets "The manufacturer’s protocol for RayBiotech Human RTK Phosphorylation Array C1 kit was followed." (see more)	ACTIV Phospho-proteomics INCU MUTA ACT-A × MORE IMAGES FROM THIS ARTICLE Elevated phosphorylation of EGFR in NSCLC due to mutations in PTPRH bioRxiv Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Elevated phosphorylation of EGFR in NSCLC due to mutations in PTPRH bioRxiv 2000 Jan 01 Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Human RTK Phosphorylation Array C1 kit RayBiotech Article Snippet "The manufacturer’s protocol for RayBiotech Human RTK Phosphorylation Array C1 kit was followed." Figure Legend "Figure 6: PTPRH Regulation of Receptor Tyrosine Kinases outside the ERBB Family Ablation of PTPRH results in differential activation of numerous receptor tyrosine kinases outside of EGFR. A) A human phosphorylated RTK array shows differential phosphorylation of numerous RTKs when incubated with lysate from H23 PTPRH WT cells. B) A human phosphorylated RTK array shows differential phosphorylation of numerous RTKs when incubated with lysate from H23 PTPRH KO cells. Top five differentially phosphorylated RTKs are highlighted in red. C) Table showing the RTKs with the top 5 largest fold changes between PTPRH KO and PTPRH WT lysates. D) The lollipop plot in was recreated, adding predicted activation for FGFR1 and IGF-1R. Briefly, each dot on the PTPRH exome plot corresponds to a PTPRH mutant NSCLC tumor. Color-coded bars above each dot correspond to the predicted activity of EGFR, FGFR1, or IGF-1R. E) Western blotting confirms increased phosphorylation of FGFR1 at tyrosine residues 653/654, within PTPRH KO cell lysates compared to PTPRH WT lysates. " ACTIV Phospho-proteomics INCU MUTA ACT-A MORE IMAGES FROM THIS ARTICLE bioRxiv Elevated phosphorylation of EGFR in NSCLC due to mutations in PTPRH