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Image Search Results
Journal: mSystems
Article Title: Transcriptional Landscape of Ectomycorrhizal Fungi and Their Host Provides Insight into N Uptake from Forest Soil
doi: 10.1128/mSystems.00957-21
Figure Lengend Snippet: Relative abundance of root-associated fungi (RAF) based on ITS2 barcoding (A), raw counts of the metabolically active fungi based on RNA sequencing characterized by taxonomy (B), and Pearson correlations between DNA-based and RNA-based abundances of the fungal genera (C). RAF were studied on roots of European beech ( Fagus sylvatica ) grown in native forest soil and treated with either water (control), ammonium, or nitrate for 2 days before harvest ( n = 4 per treatment).
Article Snippet:
Techniques: Metabolic Labelling, RNA Sequencing, Control
Journal: RNA
Article Title: Improved RNA preservation for immunolabeling and laser microdissection
doi: 10.1261/rna.1733509
Figure Lengend Snippet: Effect of buffer conditions on the integrity of ribosomal RNA (rRNA). (A) Electropherogram showing rRNA integrity for fresh-frozen tissue (black line), which represents the best quality RNA, or for brain sections that were ethanol-fixed and then incubated in either PBS for 5 min (hatched line) or overnight (o/n) in 2M NaCl PBS (white line). To quantify the effect of buffer condition on rRNA, the electropherogram was divided into four regions for comparison: (B) the 28S region; (C) the pre-18S region; (D) the 18S region; (E) the Inter region (see Materials and Methods for details). 28S rRNA intactness is considered a sensitive indicator of RNA integrity in general. Note, even a PBS incubation of only 5 min duration resulted in a 50% reduction in 28S area and a 120% increase in the pre-18S area. In contrast, overnight incubation in 2M NaCl or RNA Later resulted in markedly less degradation. Data are the means (+SEM). * P < 0.05, ** P < 0.01, *** P < 0.001; n ≥ 5.
Article Snippet: [ PubMed ] Schroeder A, Mueller O, Stocker S, Salowsky R,
Techniques: Incubation, Comparison