Journal: Journal of Clinical Microbiology

Article Title: Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry To Identify Vancomycin-Resistant Enterococci and Investigate the Epidemiology of an Outbreak

doi: 10.1128/jcm.01000-12

Figure Lengend Snippet: figure 5. It was clear that vanA positive E. faecium and vanB positive E. faecium 430

Article Snippet: A non VRE E. faecium reference strain ATCC 19434.

Techniques:

Journal: Advances in Experimental Medicine and Biology

Article Title: Advances in Microbiology, Infectious Diseases and Public Health

doi: 10.1007/978-3-030-35469-5

Figure Lengend Snippet: Fig. 3 Stability of the coleoptericins and coleoptericin-like AMPs Col1, Col6, and ColLC in plasma. Values indicate the percent hydrolysis of the H. axyridis peptides in (a) human, (b) mouse, and (c) rat plasma

Article Snippet: Profiling of Coleoptericins 55 Appendix Table S1 Overview of the test strains and their culture conditions Test strain Properties Culture medium Temperature [ C] Gram-negative bacteria E. coli ATCC 25922 Type strain CAMB 37 E. coli RKI 131/08 Clinical isolate CAMB 37 E. coli RKI 6A-6 Clinical isolate CAMB 37 K. pneumoniae DSM 30104 Type strain CAMB 37 K. pneumoniae RKI 93/10 Clinical isolate CAMB 37 K. pneumoniae RKI 19/16 Clinical isolate CAMB 37 P. aeruginosa ATCC 27853 Type strain CAMB 37 P. aeruginosa RKI 93/12 Clinical isolate CAMB 37 A. baumannii ATCC 19606 Quality control strain CAMB 37 A. baumannii RKI 19/09 Clinical isolate CAMB 37 P. mirabilis DSM 4479 Type strain CAMB 37 Gram-positive bacteria S. aureus ATCC 25923 MSSA CAMB 37 S. aureus ATCC 33592 MRSA CAMB 37 S. epidermidis ATCC 35984 Clinical isolate CAMB 37 E. faecium DSM 17050 VRE CAMB 37 L. monocytogenes DSM 20600 Type strain CAMB 37 M. smegmatis ATCC 607 Wild type BHI + 1% Tween-80 37 C. albicans FH2173 Wild type CAMB 28 CAMB cation-adjusted Mueller-Hinton broth, BHI brain heart infusion medium, MSSA methicillin-sensitive S. aureus, MRSA methicillin-resistant S. aureus, VRE vancomycin-resistant enterococci Table S2 Activity of the H. axyridis AMPs against reference strains Strain MIC (μg/ml) Col1 Col6 ColLC S. aureus ATCC 25923 >1024 >1024 >1024 S. aureus ATCC 33592 >1024 nd >1024 S. epidermidis ATCC 35984 >1024 nd >1024 E. faecium DSM 17050 >1024 nd >1024 L. monocytogenes DSM 20600 >1024 nd >1024 E. coli ATCC 25922 >1024 32 >1024 K. pneumoniae DSM 30104 512 32 >1024 A. baumannii ATCC 19606 512 32 >1024 P. aeruginosa ATCC 27853 512 256 >1024 P. mirabilis DSM 4479 >1024 nd >1024 M. smegmatis ATCC 607 >1024 nd >1024 C. albicans FH2173 >1024 nd >1024 MIC values were determined in cation-adjusted Mueller-Hinton broth (CAMB) nd not determined 56 R. Hirsch et al.

Techniques: Clinical Proteomics

Journal: Applied and Environmental Microbiology

Article Title: Mode of Action of the Antimicrobial Peptide Aureocin A53 from Staphylococcus aureus

doi: 10.1128/aem.68.11.5274-5280.2002

Figure Lengend Snippet: FIG. 2. Influence of aureocin A53 on macromolecular synthesis (indicator strain, S. simulans 22). Shown is the uptake of [3H]glucose (A), [3H]thymidine (B), and [3H]glutamate (C) into the cellular mac- romolecules by S. simulans 22. E, untreated cells; F, cells treated with aureocin A53 at 10 times the MIC. The arrows indicate the points of addition of bacteriocin.

Article Snippet: MICs of aureocin A53 for various gram-positive bacteria Organism Strain Description MIC ( g/ml) Reference E. faecium BM4147 Vancomycin resistant 0.29 17 L. innocua ATCC 33090 Apathogenic 0.59 M. luteus ATCC 4698 Aureocin A53 sensitive 0.87 10 3 S. aureus LT-1334a Methicillin-resistant isolate 1.17 S. aureus J-11574a Methicillin-susceptible isolate 1.17 Staphylococcus epidermidis LT-1324a Methicillin-resistant isolate 0.59 S. simulans 22 Aureocin A53 sensitive 0.59 26 a Strain isolated in the Institute for Medical Microbiology and Immunology of the University Bonn, Bonn, Germany. on M ay 19, 2015 by U N IV O F C O N N E C T IC U T http://aem .asm .org/ D ow nloaded from and thereby activated, cell wall-lytic enzymes from anionic polymers (teichoic and lipoteichoic acids).

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Mode of Action of the Antimicrobial Peptide Aureocin A53 from Staphylococcus aureus

doi: 10.1128/aem.68.11.5274-5280.2002

Figure Lengend Snippet: FIG. 3. Effect of aureocin A53 on membrane potential of S. simu- lans 22. , control without bacteriocin; ‚, aureocin A53 at 0.1 MIC; E, aureocin A53 at MIC; F, aureocin A53 at 10 times MIC. The arrow indicates the moment of addition of aureocin A53.

Article Snippet: MICs of aureocin A53 for various gram-positive bacteria Organism Strain Description MIC ( g/ml) Reference E. faecium BM4147 Vancomycin resistant 0.29 17 L. innocua ATCC 33090 Apathogenic 0.59 M. luteus ATCC 4698 Aureocin A53 sensitive 0.87 10 3 S. aureus LT-1334a Methicillin-resistant isolate 1.17 S. aureus J-11574a Methicillin-susceptible isolate 1.17 Staphylococcus epidermidis LT-1324a Methicillin-resistant isolate 0.59 S. simulans 22 Aureocin A53 sensitive 0.59 26 a Strain isolated in the Institute for Medical Microbiology and Immunology of the University Bonn, Bonn, Germany. on M ay 19, 2015 by U N IV O F C O N N E C T IC U T http://aem .asm .org/ D ow nloaded from and thereby activated, cell wall-lytic enzymes from anionic polymers (teichoic and lipoteichoic acids).

Techniques: Membrane, Control

Journal: Applied and Environmental Microbiology

Article Title: Mode of Action of the Antimicrobial Peptide Aureocin A53 from Staphylococcus aureus

doi: 10.1128/aem.68.11.5274-5280.2002

Figure Lengend Snippet: FIG. 4. (A) Accumulation of [3H]glutamate by S. simulans 22 ( ) and efflux upon addition of aureocin A53 at MIC (E) and 10 times MIC (F). (B) Accumulation of 86Rb by S. simulans 22 ( ) and efflux of 86Rb upon addition of aureocin A53 at MIC (E) and 10 times MIC (F). The arrows indicate the addition of peptide.

Article Snippet: MICs of aureocin A53 for various gram-positive bacteria Organism Strain Description MIC ( g/ml) Reference E. faecium BM4147 Vancomycin resistant 0.29 17 L. innocua ATCC 33090 Apathogenic 0.59 M. luteus ATCC 4698 Aureocin A53 sensitive 0.87 10 3 S. aureus LT-1334a Methicillin-resistant isolate 1.17 S. aureus J-11574a Methicillin-susceptible isolate 1.17 Staphylococcus epidermidis LT-1324a Methicillin-resistant isolate 0.59 S. simulans 22 Aureocin A53 sensitive 0.59 26 a Strain isolated in the Institute for Medical Microbiology and Immunology of the University Bonn, Bonn, Germany. on M ay 19, 2015 by U N IV O F C O N N E C T IC U T http://aem .asm .org/ D ow nloaded from and thereby activated, cell wall-lytic enzymes from anionic polymers (teichoic and lipoteichoic acids).

Techniques: