Journal: Cell Communication and Signaling : CCS

Article Title: A novel highly selective allosteric inhibitor of tyrosine kinase 2 (TYK2) can block inflammation- and autoimmune-related pathways

doi: 10.1186/s12964-023-01299-7

Figure Lengend Snippet: QL-1200186 blocks the TYK2 signaling pathway. A Effect of QL-1200186 on the JAK/STAT/ISGF3 pathway. B Human PBMCs were stimulated with human IFNα and pSTAT5 levels were determined. C Th17 cells were stimulated with IL-23 and pSTAT3 levels were determined. D IL-12 induced IFNγ production in NK92 cells. Data are the mean ± SD. At least three independent experiments were carried out

Article Snippet: With regard to IFNα-stimulated phosphorylation of STAT5 in human peripheral blood mononuclear cells (PBMCs), after incubation with QL-1200186 for 1 h, human PBMCs were stimulated with recombinant human IFNα (1000 U/mL; 11200–2; PBL Assay Science) for 30 min. With respect to GM-CSF-stimulated JAK2 activation in U937 cells (Cobioer Biosciences, CBP60277), after 1 h of treatment with QL-1200186, U937 cells were stimulated with GM-CSF (20 ng/mL; C003; Novoprotein, Suzhou, China) for 15 min. With regard to IL-2-stimulated JAK1/3 activation in human PBMCs, after 1 h of incubation with QL-1200186, human PBMCs were stimulated with human IL-2 (20 ng/mL; C013; Novoprotein) for 20 min. With respect to IL-2-stimulated JAK1/3 activation in human whole blood cells, whole blood (50 μL) was preincubated with compounds for 30 min and then stimulated with IL-2 (20 ng/mL) for 15 min.

Techniques:

Journal: Cell Communication and Signaling : CCS

Article Title: A novel highly selective allosteric inhibitor of tyrosine kinase 2 (TYK2) can block inflammation- and autoimmune-related pathways

doi: 10.1186/s12964-023-01299-7

Figure Lengend Snippet: Inhibitory function and selectivity of QL-1200186 compared with other TYK2 inhibitors. A Effect of TYK2 inhibitors on IFNα-induced pTYK2 expression. B , C Effects of TYK2 inhibitors on pSTAT1 expression in CD3 + T and CD19 + B cells ( n = 3). D Effects of TYK2 inhibitors on IL-12-induced IFNγ production in human whole blood cells ( n = 3). E Effects of TYK2 inhibitors on the differentiation of monocytes into dendritic cells ( n = 2). F Effect of a TYK2 inhibitor and tofacitinib on the activity of JAK 1/3 was detected by IL-2-induced pSTAT5 expression ( n = 3). G , H Effect of compounds on JAK 2/2 activity was determined by thrombopoietin-induced pSTAT3/5 expression ( n = 3)

Article Snippet: With regard to IFNα-stimulated phosphorylation of STAT5 in human peripheral blood mononuclear cells (PBMCs), after incubation with QL-1200186 for 1 h, human PBMCs were stimulated with recombinant human IFNα (1000 U/mL; 11200–2; PBL Assay Science) for 30 min. With respect to GM-CSF-stimulated JAK2 activation in U937 cells (Cobioer Biosciences, CBP60277), after 1 h of treatment with QL-1200186, U937 cells were stimulated with GM-CSF (20 ng/mL; C003; Novoprotein, Suzhou, China) for 15 min. With regard to IL-2-stimulated JAK1/3 activation in human PBMCs, after 1 h of incubation with QL-1200186, human PBMCs were stimulated with human IL-2 (20 ng/mL; C013; Novoprotein) for 20 min. With respect to IL-2-stimulated JAK1/3 activation in human whole blood cells, whole blood (50 μL) was preincubated with compounds for 30 min and then stimulated with IL-2 (20 ng/mL) for 15 min.

Techniques: Expressing, Activity Assay

Journal: Cell Reports Methods

Article Title: Generation, expansion, gene delivery, and single-cell profiling in rhesus macaque plasma B cells

doi: 10.1016/j.crmeth.2024.100878

Figure Lengend Snippet:

Article Snippet: IFN alpha 2B , Proteintech , HZ-1072.

Techniques: Enzyme-linked Immunospot, Isolation, Enzyme-linked Immunosorbent Assay, Recombinant, Software