| | | Article Snippets "Antibodies against the following proteins were used: PY397 FAK/PTK2 (Cell Signaling Danvers, MA, USA; 8556P), FAK (Cell Signaling 13009P), β-actin/ACTB (Merck Sigma-Aldrich A5441), βIII-tubulin/TUBB3 (Covance, .." (see more) | ×![]() Product associated with above image that matches your search query Cell Signaling Technology  Article Snippet "Antibodies against the following proteins were used: PY397 FAK/PTK2 (Cell Signaling Danvers, MA, USA; 8556P), FAK (Cell Signaling 13009P), β-actin/ACTB (Merck Sigma-Aldrich A5441), βIII-tubulin/TUBB3 (Covance, Princetown, New Jersey, USA; PRB-435P), GFAP (Millipore, Burlington, MA, USA; AB5804), Ki67/MKI67 (Santa Cruz Biotechnology sc-15402), Lamin B1/LMNB1 (Calbiochem, San Diego, CA, USA; NA12), p27 (Genetex, Alton Pkwy Irvine, CA, USA; GTX100446-25), p62 (Novus Biologicals, Centennial, CO, USA; NBP1-48320), and SKP-2 (Abcam, Cambridge, UK; ab19877)." Figure Legend "Figure 1 Inhibition of focal adhesion kinase (FAK) reshapes glioblastoma (GBM) cell morphology and increases cell size. ( A ) Cell lysates from different GBM cell lines (A172, U251-MG, U87-MG, and T98G) were analyzed for PY397 FAK and total FAK. β-actin was used as a loading control. GBM cell lines display active PY397 FAK, with U251-MG and U87-MG showing the highest levels. ( B ) U251-MG and U87-MG cell lysates (control or treated with PF-573228 10 µM) were analyzed for active and total FAK. β-actin was used as a loading control. FAK inhibitor effectively reduced PY397 FAK levels. ( C ) Glial Fibrillary Acidic Protein (GFAP), βIII-tubulin, and Lamin B1 immunostainings performed in U251-MG cells (after 4–5 days of treatment with PF-573228 10 µM). Cytoskeleton remodeling accompanied by cell body enlargement and lobulated/enlarged nuclei is revealed by Lamin B1 immunostaining. Bars = 28 µm. " | |
