Journal: Cell Reports Medicine

Article Title: CD103 + CD8 + T RM Cells Accumulate in Tumors of Anti-PD-1-Responder Lung Cancer Patients and Are Tumor-Reactive Lymphocytes Enriched with Tc17

doi: 10.1016/j.xcrm.2020.100127

Figure Lengend Snippet:

Article Snippet: anti-human pSTAT3pS727-APC (REA324) , Miltenyi Biotec , Cat# 130-105-000; RRID: AB_2653576.

Techniques: Purification, Recombinant, Isolation, Staining, RNA Library Preparation, cDNA Synthesis, SYBR Green Assay, Enzyme-linked Immunosorbent Assay, Software

Journal: bioRxiv

Article Title: G1899, an American ginseng extract, alleviates neuroinflammation and cognitive impairment in models of Alzheimer’s disease

doi: 10.1101/2025.09.04.674314

Figure Lengend Snippet: G1899 promotes microglial homeostasis and attenuates detrimental microglial activation induced by amyloid β oligomer (Aβo). a Schematic illustration (created with BioRender.com) of the treatment time window for G1899 and Aβo in mouse primary microglia. b Representative immunoblot images showing dose-dependent upregulation of anti-TMEM119 (homeostatic marker) and anti-CD68 (phagocytic marker) in G1899-treated microglia. Quantification of c TMEM119 and d CD68 normalized to β-actin. e Effects of G1899 on NLRP3 inflammasome formation and STAT3 signaling, with quantification of f NLRP3, g Caspase-1, h ASC, i pY705 STAT3, j pS727 STAT3, and k total STAT3 normalized to β-actin. l Immunofluorescence analysis of morphological changes using anti-TMEM119 and anti-Iba1 staining. Scale bar, 20 μm. m Quantification of cellular area following Aβo exposure, demonstrating restoration of microglial morphology by G1899. n Representative immunoblot images of anti-iNOS (activation marker), anti-TMEM119, and β-actin with quantification of o iNOS and p TMEM119 normalized to β-actin. Error bars indicate mean ± SEM. Statistical significance is determined by one-way ANOVA (c, d, f, g, i-p) or two-way ANOVA (h) with Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; n.s., not significant.

Article Snippet: Biolegend, #137001), anti- CD68 (1:1000, CST, #9778S), anti Caspase1 (1:500, Adipogen Life Sciences, AG-20B-0044- C100), anti-ASC (1:1000, Adipogen Life Sciences, AG-25B-0006), anti-Iba1 (1:1000, Wako, #019- 19741), anti-pY705 STAT3 (1:1000, CST, #9145S), anti-pS727 STAT3 (1:1000, CST, #9134S), anti-total STAT3 (1:1000, CST, #9139S), anti-Presenilin1 (1:1000, CST, #3622S), anti-APP (1:1000, MilliporeSigma, #MAB348), anti-Syntaxin 1A (1:1000, Biolegend, #827001), anti-IL-1β (1:1000, CST, #12242S), anti-Aβ (4G8) (1:1,000, Biolegend, #800708), anti-Aβ (6E10) (1:1,000, Biolegend, #803014) or anti-β-actin (1:5000, Sigma, #A3854-200UL) followed by HRP- conjugated rabbit (#32260) or mouse (#32230) secondary antibodies (1:5000, Thermo) for 1 hr at room temperature.

Techniques: Activation Assay, Western Blot, Marker, Immunofluorescence, Staining

Journal: bioRxiv

Article Title: G1899, an American ginseng extract, alleviates neuroinflammation and cognitive impairment in models of Alzheimer’s disease

doi: 10.1101/2025.09.04.674314

Figure Lengend Snippet: Attenuation of hippocampal neuroinflammation by G1899 in 5xFAD/+ mice. a Representative immunoblot images showing expression of Aβ, IL-1β, caspase-1, pY705 STAT3, Iba1, and Syntaxin 1A in the hippocampus of WT, 5xFAD/+, and 5xFAD/+ mice treated with 300 mg/kg G1899. Quantification of relative protein expression of b Aβ (6E10), c IL-1β, d caspase-1, e pY705 STAT3, f Iba1, and g Syntaxin 1A. h Representative immunofluorescence images detecting Aβ (4G8) co-stained with Iba1 in the dorsal subiculum of the hippocampus, shown at 10× magnification for global Iba1 intensity (Scale bar, 100 μm) and i 63× magnification to visualize microglial recruitment toward Aβ plaques (Scale bar, 10 μm). Quantification of j Iba1 immunofluorescence intensity and k the number of microglia recruited toward Aβ plaques. Error bars indicate mean ± SEM. Statistical significance is determined by one-way ANOVA followed by Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; n.s., not significant; n.d., not detected.

Article Snippet: Biolegend, #137001), anti- CD68 (1:1000, CST, #9778S), anti Caspase1 (1:500, Adipogen Life Sciences, AG-20B-0044- C100), anti-ASC (1:1000, Adipogen Life Sciences, AG-25B-0006), anti-Iba1 (1:1000, Wako, #019- 19741), anti-pY705 STAT3 (1:1000, CST, #9145S), anti-pS727 STAT3 (1:1000, CST, #9134S), anti-total STAT3 (1:1000, CST, #9139S), anti-Presenilin1 (1:1000, CST, #3622S), anti-APP (1:1000, MilliporeSigma, #MAB348), anti-Syntaxin 1A (1:1000, Biolegend, #827001), anti-IL-1β (1:1000, CST, #12242S), anti-Aβ (4G8) (1:1,000, Biolegend, #800708), anti-Aβ (6E10) (1:1,000, Biolegend, #803014) or anti-β-actin (1:5000, Sigma, #A3854-200UL) followed by HRP- conjugated rabbit (#32260) or mouse (#32230) secondary antibodies (1:5000, Thermo) for 1 hr at room temperature.

Techniques: Western Blot, Expressing, Immunofluorescence, Staining

Journal: bioRxiv

Article Title: G1899, an American ginseng extract, alleviates neuroinflammation and cognitive impairment in models of Alzheimer’s disease

doi: 10.1101/2025.09.04.674314

Figure Lengend Snippet: Suppression of inflammatory activation by G1899 in human induced microglia (iMG). a Representative immunoblot images showing expression of Oct-4a, Nanog, CD34, CD68, TMEM119, Iba1, and β-actin during stepwise stages of iMG differentiation. Quantification of b Oct-4a, c Nanog, d CD34, e CD68, f TMEM119, and g Iba1 expression across differentiation stages. h Representative immunoblot images showing expression of NLRP3, IL-1β, STAT3, pY705 STAT3, pS727 STAT3, Iba1, Aβ (6E10 and 4G8), and β-actin. Quantification of i NLRP3, j IL-1β, k STAT3, l pY705 STAT3, m pS727 STAT3, n Iba1, o Aβ (6E10), and p Aβ (4G8). Q Representative immunoblot images showing expression of TMEM119, CD68, Iba1, and β-actin, with quantification of r TMEM119 and s CD68. t Representative immunofluorescence images of iMG co-stained with Iba1 and TMEM119. Scale bar, 10 μm. u Quantification of relative cell size in . Error bars represent mean ± SEM. Statistical significance was determined by one-way or two-way ANOVA followed by Tukey’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001; n.s., not significant.

Article Snippet: Biolegend, #137001), anti- CD68 (1:1000, CST, #9778S), anti Caspase1 (1:500, Adipogen Life Sciences, AG-20B-0044- C100), anti-ASC (1:1000, Adipogen Life Sciences, AG-25B-0006), anti-Iba1 (1:1000, Wako, #019- 19741), anti-pY705 STAT3 (1:1000, CST, #9145S), anti-pS727 STAT3 (1:1000, CST, #9134S), anti-total STAT3 (1:1000, CST, #9139S), anti-Presenilin1 (1:1000, CST, #3622S), anti-APP (1:1000, MilliporeSigma, #MAB348), anti-Syntaxin 1A (1:1000, Biolegend, #827001), anti-IL-1β (1:1000, CST, #12242S), anti-Aβ (4G8) (1:1,000, Biolegend, #800708), anti-Aβ (6E10) (1:1,000, Biolegend, #803014) or anti-β-actin (1:5000, Sigma, #A3854-200UL) followed by HRP- conjugated rabbit (#32260) or mouse (#32230) secondary antibodies (1:5000, Thermo) for 1 hr at room temperature.

Techniques: Activation Assay, Western Blot, Expressing, Immunofluorescence, Staining