Journal: Scandinavian Journal of Medicine & Science in Sports

Article Title: Alpha-2 adrenergic stimulation triggers Achilles tenocyte hypercellularity: Comparison between two model systems

doi: 10.1111/j.1600-0838.2011.01442.x

Figure Lengend Snippet: Section of rabbit Achilles tendon tissue processed with immunohistochemistry (a, b; tetramethylrhodamine isothiocyanate staining) and in situ hybridization (inset in b; anti-digoxigenin alkaline phosphatase immunostaining detection), for detection of tyrosine hydroxylase (TH; a), alpha-2A-adrenoreceptor (α 2A AR; b) and TH mRNA (inset in b). (a) TH-positive nerve structures are seen in the paratendinous tissue in the form of perivascular nerves (arrows) and in a nerve fascicle (N). Asterisk marks lumen of vessel. (b) Tenocytes of the tendon tissue proper display immunoreactions for α 2A AR (arrows). (Inset) A tenocyte is positive for TH mRNA (arrow). Antisense staining.

Article Snippet: For control purposes, a negative control using the corresponding sense DIG-hyperlabeled ssDNA probe was used, and positive controls in the form of a Poly(dT) probe (GD4000-OP; GeneDetect) and a β-actin antisense probe (GD5000-OP, GeneDetect) were used.

Techniques: Immunohistochemistry, Staining, In Situ Hybridization, Immunostaining

Journal: Investigative Ophthalmology & Visual Science

Article Title: Ornithine-δ-Aminotransferase Inhibits Neurogenesis During Xenopus Embryonic Development

doi: 10.1167/iovs.15-16509

Figure Lengend Snippet: Spatio-temporal expression of OAT during embryonic development. Whole-mount in situ hybridization analysis shows that (A) X-OAT is expressed in all regions of oocyte. (B) In the early neural stage, X-OAT is localized principally to the dorsal side, especially in the notoplate (white arrow). (C) The X-OAT is predominantly expressed in brain and spinal cord at tailbud stage, as shown with digoxigenin-labeling (white arrow).

Article Snippet: In situ hybridization studies were performed using a digoxigenin-labeled (Boehringer-Ingelheim, Ingelheim, Germany) antisense RNA probe or a fluorescein-labeled (Boehringer-Ingelheim) antisense RNA probe.

Techniques: Expressing, In Situ Hybridization, Labeling

Journal: The Journal of Neuroscience

Article Title: Neuregulins Signaling via a Glial erbB-2–erbB-4 Receptor Complex Contribute to the Neuroendocrine Control of Mammalian Sexual Development

doi: 10.1523/JNEUROSCI.19-22-09913.1999

Figure Lengend Snippet: Detection of erbB-2 and erbB-4 mRNAs and absence of erbB-3 mRNA in isolated hypothalamic (Hypo) and cerebrocortical (CTX) astrocytes (Astro.), as assessed by RNase protection assay. M,32P-UTP-labeled RNA molecular size marker;UP, undigested cRNA probes; DP, digested probes; cyclo, cyclophilin.

Article Snippet: In brief, 32 P-UTP-labeled rat erbB-2 and erbB-4 antisense RNA probes were purified using a Fullengther apparatus (Biokey Co., Portland, OR), as recommended ( Ma et al., 1996 ).

Techniques: Isolation, Rnase Protection Assay, Labeling, Marker

Journal: The Journal of Neuroscience

Article Title: Neuregulins Signaling via a Glial erbB-2–erbB-4 Receptor Complex Contribute to the Neuroendocrine Control of Mammalian Sexual Development

doi: 10.1523/JNEUROSCI.19-22-09913.1999

Figure Lengend Snippet: Cellular localization of erbB-4 and erbB-2 mRNAs in the brain of peripubertal female rats, as detected by in situ hybridization using 35S-UTP-labeled cRNA probes. Within hypothalamic nuclei, the mRNA was found to be more abundant in cells of the paraventricular nuclei (A,arrows) and the ARC, VMH, and DMH nuclei (B). ErbB-4 mRNA is also diffusely detected throughout the medial basal hypothalamus (B,C) and is more abundantly present in cells of the subependymal region (A, B, small arrowheads) and glial cells in the intermediate and external layers of the median eminence (B and C,small and large arrowheads, respectively). ErbB-2 transcripts were more abundantly expressed in ependymal cells lining the third ventricle (E,F, arrowheads) and glial cells of the median eminence (F, arrows).D and G depict sections adjacent toC and F hybridized with sense erbB-4 (D) and erbB-2 (G) RNA probes. Scale bars: B, 200 μm; A,C–G, 100 μm.

Article Snippet: In brief, 32 P-UTP-labeled rat erbB-2 and erbB-4 antisense RNA probes were purified using a Fullengther apparatus (Biokey Co., Portland, OR), as recommended ( Ma et al., 1996 ).

Techniques: In Situ Hybridization, Labeling