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Image Search Results
Journal: Advanced Science
Article Title: The PRMT6/PARP1/CRL4B Complex Regulates the Circadian Clock and Promotes Breast Tumorigenesis
doi: 10.1002/advs.202202737
Figure Lengend Snippet: Assembly sequence of the PRMT6/PARP1/CRL4B complex. A) Lysates from PRMT6‐knockdown MDA‐MB‐231 cells immunoprecipitated with control IgG or an anti‐PARP1 antibody, followed by immunoblotting with indicated antibodies. B) Lysates from PARP1‐knockdown MDA‐MB‐231 cells immunoprecipitated with control IgG or anti‐PRMT6 antibody, followed by immunoblotting with indicated antibodies. C) Lysates from CUL4B‐knockdown MDA‐MB‐231 cells immunoprecipitated with control IgG or an anti‐PRMT6 antibody, followed by immunoblotting with indicated antibodies. D) Lysates from DDB1‐knockdown MDA‐MB‐231 cells immunoprecipitated with control IgG or an anti‐PRMT6 antibody, followed by immunoblotting with indicated antibodies. E) Lysates from MDA‐MB‐231 cells with Olaparib treatment immunoprecipitated with control IgG or an anti‐PRMT6 antibody, followed by immunoblotting with indicated antibodies. F) Lysates from MDA‐MB‐231 cells with Olaparib treatment immunoprecipitated with control IgG or an anti‐PARP1 antibody, followed by immunoblotting with indicated antibodies. G) Lysates from PRMT6‐overexpressing PRMT6 MDA‐MB‐231 cells treated with EPZ020411 immunoprecipitated with control IgG or an anti‐PARP1 antibody, followed by immunoblotting with indicated antibodies. H) Lysates from MDA‐MB‐231 cells immunoprecipitated with control IgG or an anti‐PARP1 antibody, followed by immunoblotting with indicated antibodies. I) Western blot analysis of the indicated chromatin modifications in stably overexpressing PRMT6 MDA‐MB‐231 cells. J) Schematic diagram depicting the assembled sequence of the PRMT6/PARP1/CRL4B complex.
Article Snippet: The antibodies were listed as follows: anti‐FLAG (F1408), anti‐CUL4B (C9995), anti‐ β ‐actin (A1978), anti‐HDAC1 (H3284), anti‐HDAC2 (H3159), anti‐Vimentin (V6630) and anti‐Fibronectin (F3648) from Sigma‐Aldrich; anti‐DDB1 (sc‐25367) and anti‐PER1 (sc‐398890) from Santa Cruz Biotechnology; anti‐ROC1 (ab2977), anti‐H3 (ab1791), anti‐H4 (ab17840), anti‐H3R2me2a (ab194706), anti‐H3R2me2s (ab194684), anti‐H4R3me2s (ab5823), antiH4R3me2a (ab129231), anti‐H3R8me2s (ab130740), anti‐H3R8me2a (ab127163), anti‐H3K4me2 (ab32356), anti‐H3K36me1 (ab176920), anti‐H3K36me2 (ab176921), and anti‐H3K36me3 (ab194677) from Abcam; anti‐H2AK119ub1 (05‐678) from Millipore; anti‐ α ‐catenin (610193), anti‐ γ ‐catenin (610253), anti‐E‐cadherin (610181), and anti‐N‐cadherin (610920) from BD Bioscience; anti‐PRMT6 (14641S), anti‐PARP1 (9532T), and anti‐HER2 (4290) from Cell Signaling Technology; anti‐PER3 (12550‐1‐AP), anti‐LDHA (21799‐1‐AP), anti‐BHLHE40 (17895‐1‐AP), and anti‐CSNK1A1 (55192‐1‐AP) from Proteintech;
Techniques: Sequencing, Knockdown, Immunoprecipitation, Control, Western Blot, Stable Transfection