Journal: Scientific Reports

Article Title: Structural and developmental expression of Ss-riok- 2, an RIO protein kinase encoding gene of Strongyloides stercoralis

doi: 10.1038/s41598-017-07991-2

Figure Lengend Snippet: Representative expression pattern of GFP under the putative Ss-riok-2 promoter in a transgenic second-stage larva of Strongyloides stercoralis . Differential interference contrast (DIC) and fluorescence (GFP) images showing the expression of construct S. stercoralis Ss-riok-2p::gfp (pRP2; Fig. ) in a post-free-living second-stage larva. GFP reporter expression predominated in intestine. Scale bars = 10 μm.

Article Snippet: The purified product was then subcloned into the promoter-less vector pAJ01 upstream of the gfp coding sequence (Addgene, Cambridge, Massachusetts, USA, http://www.addgene.org ) to create the plasmid pRP2 (Fig. ).

Techniques: Expressing, Transgenic Assay, Fluorescence, Construct