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All (6)
MilliporeSigma
Twist Bioscience
Azenta
GenScript
Merck KGaA
Thermo Fisher
 

About 19 products / 149 snippets

91 / 100
Bioz Stars
PET-21(+) vector
MilliporeSigma / Sigma Aldrich  
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Techniques
 
Article Snippets
"442-723), with the SSDD motif added, was inserted into pET-21(+) vector (Sigma-Aldrich)."  (see more)
80 / 100
Bioz Stars
PET-21 vector
Azenta / GENEWIZ  
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Techniques
 
Article Snippets
"CasΦ3 cDNA was synthetized and cloned with a C-terminal hexahistidine (His)-tag into pET-21 vector (Genewiz) ( )."  (see more)
91 / 100
Bioz Stars
PET-21(+) vector
Twist Bioscience  
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Techniques
 
Article Snippets
"According to [ ], the YF1/FixJ is first inserted into a pET-21(+) vector via Twist Bioscience."  (see more)
80 / 100
Bioz Stars
Vector pET- 21(+)
MilliporeSigma / Novagen  
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Techniques
 
Article Snippets
"Post capture PCR amplicons were cloned via Gibson Assembly in the vector pET- 21(+) (Novagen) that was previously linearized by PCR using tailed-primers pET21RGibson and pET21FGibson. .."  (see more)
91 / 100
Bioz Stars
PET-21(+) vector
Thermo Fisher Scientific  
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Techniques
 
Article Snippets
"Unless indicated, designs were expressed from a pET-21(+) vector ( Thermo Fisher ) with an N-terminal 6xHis tag and TEV cleavage sequence."  (see more)
94 / 100
Bioz Stars
PET-21(a) vector
MilliporeSigma / Novagen  
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Techniques
 
Article Snippets
"Then the NdeI-BamHI fragment was excised and cloned into the pET-21(a) vector (Novagen) to construct pET21a- sufE ."  (see more)
80 / 100
Bioz Stars
PET 21(+a) vector
MilliporeSigma / Novagen  
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Techniques
 
Article Snippets
".. (New England Biolabs, Ipswich, MA, USA), digested with NdeI and EcoRI and cloned into the pET 21(+a) vector (Novagen, Merck KGaA, Darmstadt, Germany) resulting in the plasmid pET21GAD."  (see more)
91 / 100
Bioz Stars
PET-21-d vector
MilliporeSigma  
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Techniques
 
Article Snippets
"Residues 256–885 and 409–885 of CagA were cloned into a pET-21-d vector (EMD Millipore) to produce C-terminal hexahistidine fusion proteins that were expressed in BL21(DE3) cells at .."  (see more)
93 / 100
Bioz Stars
PET-21 b(+) vector
MilliporeSigma / Novagen  
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Techniques
 
Article Snippets
".. XhoI from NEB, purified again using a PCR-clean-up kit, and finally ligated into a pET-21 b(+) vector from Novagen that had been prepared by double-digesting with NdeI and XhoI and using a Fermentas .."  (see more)
80 / 100
Bioz Stars
PET 21-b vector
MilliporeSigma / Novagen  
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Techniques
 
Article Snippets
".. directionally cloned: pAE-6His vector for SmAP and SmNPP-5 ( ; ) and pET 21-b vector (Novagen) for SmNTPDase ( )."  (see more)
81 / 100
Bioz Stars
PET-21(1) vector
MilliporeSigma / Novagen  
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Techniques
 
Article Snippets
"The T7RP insert was subcloned into the NcoI/XhoI sites of the pET-21(1) vector (Novagen) modified to contain the EMC 59 UTR, including a 59 stem-loop structure (Fuerst and Moss, .."  (see more)
85 / 100
Bioz Stars
PET-21(+) vectors
GenScript  
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Techniques
 
Article Snippets
"The genes for these proteins were designed and ordered through GenScript in pET-21(+) vectors."  (see more)