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Image Search Results
Journal:
Article Title: Cloning and Characterization of the Bile Salt Hydrolase Genes ( bsh ) from Bifidobacterium bifidum Strains
doi: 10.1128/AEM.70.9.5603-5612.2004
Figure Lengend Snippet: Bacterial strains and plasmids used in this study
Article Snippet: When appropriate, ampicillin (200 μg/ml) and kanamycin (40 μg/ml) were added. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Origin or relevant characteristics Source or reference Bacterial strains B. bifidum ATCC 11863 Wild type for cloning ATCC B. bifidum ATCC 35914 Human feces ATCC B. bifidum ATCC 15696 Infant intestine ATCC B. bifidum ATCC 29521 Type strain; infant feces ATCC B. bifidum KL 301 Dairy isolate This study B. bifidum KL 306 Dairy isolate This study B. adolescentis ATCC 15703 Type strain; adult intestine ATCC B. longum ATCC 15707 Type strain; adult intestine ATCC B. infantis ATCC 15697 Type strain; infant intestine ATCC B. breve ATCC 15707 Type strain; infant intestine ATCC E. coli DH5α F − φ80d lacZ ΔM15 Δ( lacZYA-argF ) U169 endA1 recA1 hsdR17 (r K − m K + ) deoR GIBCO-BRL E. coli BL21(DE3) F − ompT hsdS B (r B − m B − ) gal dcm lacY1 (DE3) Novagen Plasmids pBR322 Ap r ; cloning vector Invitrogen pUC19 Ap r ; cloning vector Invitrogen pDrive Ap r Kan r ;
Techniques: Plasmid Preparation, Clone Assay, PCR Cloning, Expressing, Mutagenesis