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Image Search Results
Journal: bioRxiv
Article Title: DECIPHERING THE DARK SIDE OF HISTONE ADP-RIBOSYLATION: WHAT STRUCTURAL FEATURES OF DAMAGED NUCLEOSOME REGULATE THE ACTIVITIES OF PARP1 AND PARP2
doi: 10.1101/2025.01.29.635606
Figure Lengend Snippet: a − Typical titration curves reflecting dissociation of fluorescently labelled DNA147 or gap35-DNA167 from the complex with PARP1 upon addition of increasing concentrations of the competitor DNA (plasmid pBR322); b − CC 50 values determined for different DNA structural variants.
Article Snippet: DNA primers for synthesis of Widom603 147 bp DNA were synthesized by Lumiprobe (Moscow, Russia). pGEM-3z/603 (Addgene plasmid #26658; http://n2t.net/addgene:26658 ; RRID: Addgene_26658) was a gift from J. Widom.
Techniques: Titration, Plasmid Preparation