oc43 n Search Results


recombinant hcov-oc43 n-bio-his6 protein  (Bioneer Corporation)
90
Bioneer Corporation recombinant hcov-oc43 n-bio-his6 protein
Recombinant Hcov Oc43 N Bio His6 Protein, supplied by Bioneer Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant hcov-oc43 n-bio-his6 protein/product/Bioneer Corporation
Average 90 stars, based on 1 article reviews
recombinant hcov-oc43 n-bio-his6 protein - by Bioz Stars, 2026-04
90/100 stars
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primary antibody directed against the nucleoprotein of hcov-oc43 mab9013  (Merck KGaA)
90
Merck KGaA primary antibody directed against the nucleoprotein of hcov-oc43 mab9013
Primary Antibody Directed Against The Nucleoprotein Of Hcov Oc43 Mab9013, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary antibody directed against the nucleoprotein of hcov-oc43 mab9013/product/Merck KGaA
Average 90 stars, based on 1 article reviews
primary antibody directed against the nucleoprotein of hcov-oc43 mab9013 - by Bioz Stars, 2026-04
90/100 stars
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hcov-oc43 n  (Merck & Co)
90
Merck & Co hcov-oc43 n
(A) Multiple rounds of virus infection in 293FT WT, TMEM41B KO clone #1, and VMP1 KO clone #1 cells. (Top) Western blotting analysis to detect TMEM41B and VMP1 protein levels in the WT and KO cells. (Bottom) Crystal violet staining to visualize surviving cells after cytolytic virus infection. Non-infected controls are indicated as “mock”. (B, C) Validation of TMEM41B and VMP1 as host dependency factors across all DENV serotypes. For (B), cells were infected by either DENV1 (MOI = 1 PFU/cell), DENV2 (MOI = 0.1 PFU/cell), DENV3 (MOI = 3 PFU/cell), or DENV4 (MOI = 5 PFU/cell) for 72 hours. Accumulation of DENV NS3 proteins was detected by western blot. For (C), cells were infected at MOI of 1 for 72 hours. Released progeny virions in the supernatant were assessed by plaque assay. (D) <t>HCoV-OC43</t> protein accumulation (Left) and progeny virion levels (Right) in 293FT WT and KO cells. Cells were infected with HCoV-OC43 at MOI of 0.5 for 48 hours. Released progeny virions in the supernatants were harvested for titration by TCID 50 assay, and infected cell lysates were analyzed by western blotting. (E) Production of CHIKV progeny virions from WT and KO cells. Cells were infected with CHIKV at MOI of 0.1 for 24 hours, and released virions were harvested for titration by plaque assay. (F) cDNA complementation rescue of DENV infection. (Left) Production of DENV2 infectious particles from KO cells complemented with corresponding cDNAs, i.e., mCherry (control), TMEM41B , or VMP1 . (Right) Western blotting analysis of DENV2 NS3 accumulation in WT, KO, and cDNA-complemented cells. Cells were infected at MOI of 0.1 for 72 hours. Released virions in the supernatants were harvested for titration by plaque assay, and cell lysates were analyzed by western blotting. (G) Validation of TMEM41B and VMP1 as DENV host factors in A549 cells. (Left) Production of DENV1 infectious particles from A549 WT, TMEM41B KO clone #1, and VMP1 KO clone #1 cells complemented with corresponding cDNAs. (Right) Western blotting analysis of DENV1 NS3 accumulation in WT, KO, and cDNA-complemented cells. Cells were infected at MOI of 0.5 for 32 hours. GAPDH was used as a loading control for all blots. All data shown represent results from at least two independent experiments. Error bars represent mean +/- SEM, n = 3. * indicates p-value < 0.05 as determined by two-tailed unpaired t-test or one-way ANOVA. LOD indicates the limit of detection.
Hcov Oc43 N, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/hcov-oc43 n/product/Merck & Co
Average 90 stars, based on 1 article reviews
hcov-oc43 n - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


(A) Multiple rounds of virus infection in 293FT WT, TMEM41B KO clone #1, and VMP1 KO clone #1 cells. (Top) Western blotting analysis to detect TMEM41B and VMP1 protein levels in the WT and KO cells. (Bottom) Crystal violet staining to visualize surviving cells after cytolytic virus infection. Non-infected controls are indicated as “mock”. (B, C) Validation of TMEM41B and VMP1 as host dependency factors across all DENV serotypes. For (B), cells were infected by either DENV1 (MOI = 1 PFU/cell), DENV2 (MOI = 0.1 PFU/cell), DENV3 (MOI = 3 PFU/cell), or DENV4 (MOI = 5 PFU/cell) for 72 hours. Accumulation of DENV NS3 proteins was detected by western blot. For (C), cells were infected at MOI of 1 for 72 hours. Released progeny virions in the supernatant were assessed by plaque assay. (D) HCoV-OC43 protein accumulation (Left) and progeny virion levels (Right) in 293FT WT and KO cells. Cells were infected with HCoV-OC43 at MOI of 0.5 for 48 hours. Released progeny virions in the supernatants were harvested for titration by TCID 50 assay, and infected cell lysates were analyzed by western blotting. (E) Production of CHIKV progeny virions from WT and KO cells. Cells were infected with CHIKV at MOI of 0.1 for 24 hours, and released virions were harvested for titration by plaque assay. (F) cDNA complementation rescue of DENV infection. (Left) Production of DENV2 infectious particles from KO cells complemented with corresponding cDNAs, i.e., mCherry (control), TMEM41B , or VMP1 . (Right) Western blotting analysis of DENV2 NS3 accumulation in WT, KO, and cDNA-complemented cells. Cells were infected at MOI of 0.1 for 72 hours. Released virions in the supernatants were harvested for titration by plaque assay, and cell lysates were analyzed by western blotting. (G) Validation of TMEM41B and VMP1 as DENV host factors in A549 cells. (Left) Production of DENV1 infectious particles from A549 WT, TMEM41B KO clone #1, and VMP1 KO clone #1 cells complemented with corresponding cDNAs. (Right) Western blotting analysis of DENV1 NS3 accumulation in WT, KO, and cDNA-complemented cells. Cells were infected at MOI of 0.5 for 32 hours. GAPDH was used as a loading control for all blots. All data shown represent results from at least two independent experiments. Error bars represent mean +/- SEM, n = 3. * indicates p-value < 0.05 as determined by two-tailed unpaired t-test or one-way ANOVA. LOD indicates the limit of detection.

Journal: PLoS Pathogens

Article Title: TMEM41B and VMP1 modulate cellular lipid and energy metabolism for facilitating dengue virus infection

doi: 10.1371/journal.ppat.1010763

Figure Lengend Snippet: (A) Multiple rounds of virus infection in 293FT WT, TMEM41B KO clone #1, and VMP1 KO clone #1 cells. (Top) Western blotting analysis to detect TMEM41B and VMP1 protein levels in the WT and KO cells. (Bottom) Crystal violet staining to visualize surviving cells after cytolytic virus infection. Non-infected controls are indicated as “mock”. (B, C) Validation of TMEM41B and VMP1 as host dependency factors across all DENV serotypes. For (B), cells were infected by either DENV1 (MOI = 1 PFU/cell), DENV2 (MOI = 0.1 PFU/cell), DENV3 (MOI = 3 PFU/cell), or DENV4 (MOI = 5 PFU/cell) for 72 hours. Accumulation of DENV NS3 proteins was detected by western blot. For (C), cells were infected at MOI of 1 for 72 hours. Released progeny virions in the supernatant were assessed by plaque assay. (D) HCoV-OC43 protein accumulation (Left) and progeny virion levels (Right) in 293FT WT and KO cells. Cells were infected with HCoV-OC43 at MOI of 0.5 for 48 hours. Released progeny virions in the supernatants were harvested for titration by TCID 50 assay, and infected cell lysates were analyzed by western blotting. (E) Production of CHIKV progeny virions from WT and KO cells. Cells were infected with CHIKV at MOI of 0.1 for 24 hours, and released virions were harvested for titration by plaque assay. (F) cDNA complementation rescue of DENV infection. (Left) Production of DENV2 infectious particles from KO cells complemented with corresponding cDNAs, i.e., mCherry (control), TMEM41B , or VMP1 . (Right) Western blotting analysis of DENV2 NS3 accumulation in WT, KO, and cDNA-complemented cells. Cells were infected at MOI of 0.1 for 72 hours. Released virions in the supernatants were harvested for titration by plaque assay, and cell lysates were analyzed by western blotting. (G) Validation of TMEM41B and VMP1 as DENV host factors in A549 cells. (Left) Production of DENV1 infectious particles from A549 WT, TMEM41B KO clone #1, and VMP1 KO clone #1 cells complemented with corresponding cDNAs. (Right) Western blotting analysis of DENV1 NS3 accumulation in WT, KO, and cDNA-complemented cells. Cells were infected at MOI of 0.5 for 32 hours. GAPDH was used as a loading control for all blots. All data shown represent results from at least two independent experiments. Error bars represent mean +/- SEM, n = 3. * indicates p-value < 0.05 as determined by two-tailed unpaired t-test or one-way ANOVA. LOD indicates the limit of detection.

Article Snippet: The following primary antibodies were used to detect the presence of indicated proteins in this study: TMEM41B (HPA014946; 1:700; Sigma-Aldrich), VMP1 (12978S; 1:1000; Cell Signaling Technology), GAPDH (GTX627408; 1:5000; GeneTex) or (60004-1-Ig; 1:10000; Proteintech), DENV NS3 (GTX124252; 1:3000; GeneTex), HCoV-OC43 N (MAB9012; 1:3000; Merck), RIG-I (3743S; 1:1000; Cell Signaling Technology), MDA5 (5321S; 1:1000; Cell Signaling Technology), TBK1 (3504S; 1:1000; Cell Signaling Technology), and p-TBK1 (5483S; 1:1000; Cell Signaling Technology).

Techniques: Infection, Western Blot, Staining, Plaque Assay, Titration, Two Tailed Test

(A) Western blotting analysis to detect RIG-I, MDA5, TBK1, and p-TBK1 protein levels in 293FT WT cells, TMEM41B KO clone #1, and VMP1 KO clone #1, upon DENV infection. Cells were infected with DENV1 at MOI of 1 and lysates were harvested at 48 hours post-infection. (B) Western blotting analysis to detect RIG-I and MDA5 protein levels in 293FT WT, TMEM41B KO clone #1, and VMP1 KO clone #1 cells, upon HCoV-OC43 infection. Cells were infected with HCoV-OC43 at MOI of 0.5 and lysates were harvested at 48 hours post-infection. (C,D) IFN-β mRNA (C) and DENV RNA (D) abundance in uninfected and DENV1 infected (MOI of 1, 48 hours post-infection) WT and KO cells. Values demonstrated are relative to the expression levels in uninfected WT cells (C), or DENV infected WT cells (D). (E,F) DENV viral protein accumulation and infectious viral particles produced in 293FT WT, TMEM41B KO clone #1, and VMP1 KO clone #1 cells, upon further knocking out of RIG-I and MDA5. Infection assays were carried out at MOI of 1 and (E) the amount of infectious virus in the supernatant was determined at 48 hours post-infection by plaque assay, and (F) protein levels of the indicated proteins were determined by western blotting analysis. GAPDH was used as a loading control for all blots. 18S rRNA was used as the control for the RT-qPCR experiments. All data shown represent results from at least two independent experiments. Single-dashed lines divide blots from two gels loaded with the same set of lysates. Double-dashed lines divide blots from gels belonging to separate experiments using the same experimental conditions. Error bars represent mean +/- SEM, n = 3. * indicates p-value < 0.05 as determined by one-way ANOVA. LOD indicates the limit of detection.

Journal: PLoS Pathogens

Article Title: TMEM41B and VMP1 modulate cellular lipid and energy metabolism for facilitating dengue virus infection

doi: 10.1371/journal.ppat.1010763

Figure Lengend Snippet: (A) Western blotting analysis to detect RIG-I, MDA5, TBK1, and p-TBK1 protein levels in 293FT WT cells, TMEM41B KO clone #1, and VMP1 KO clone #1, upon DENV infection. Cells were infected with DENV1 at MOI of 1 and lysates were harvested at 48 hours post-infection. (B) Western blotting analysis to detect RIG-I and MDA5 protein levels in 293FT WT, TMEM41B KO clone #1, and VMP1 KO clone #1 cells, upon HCoV-OC43 infection. Cells were infected with HCoV-OC43 at MOI of 0.5 and lysates were harvested at 48 hours post-infection. (C,D) IFN-β mRNA (C) and DENV RNA (D) abundance in uninfected and DENV1 infected (MOI of 1, 48 hours post-infection) WT and KO cells. Values demonstrated are relative to the expression levels in uninfected WT cells (C), or DENV infected WT cells (D). (E,F) DENV viral protein accumulation and infectious viral particles produced in 293FT WT, TMEM41B KO clone #1, and VMP1 KO clone #1 cells, upon further knocking out of RIG-I and MDA5. Infection assays were carried out at MOI of 1 and (E) the amount of infectious virus in the supernatant was determined at 48 hours post-infection by plaque assay, and (F) protein levels of the indicated proteins were determined by western blotting analysis. GAPDH was used as a loading control for all blots. 18S rRNA was used as the control for the RT-qPCR experiments. All data shown represent results from at least two independent experiments. Single-dashed lines divide blots from two gels loaded with the same set of lysates. Double-dashed lines divide blots from gels belonging to separate experiments using the same experimental conditions. Error bars represent mean +/- SEM, n = 3. * indicates p-value < 0.05 as determined by one-way ANOVA. LOD indicates the limit of detection.

Article Snippet: The following primary antibodies were used to detect the presence of indicated proteins in this study: TMEM41B (HPA014946; 1:700; Sigma-Aldrich), VMP1 (12978S; 1:1000; Cell Signaling Technology), GAPDH (GTX627408; 1:5000; GeneTex) or (60004-1-Ig; 1:10000; Proteintech), DENV NS3 (GTX124252; 1:3000; GeneTex), HCoV-OC43 N (MAB9012; 1:3000; Merck), RIG-I (3743S; 1:1000; Cell Signaling Technology), MDA5 (5321S; 1:1000; Cell Signaling Technology), TBK1 (3504S; 1:1000; Cell Signaling Technology), and p-TBK1 (5483S; 1:1000; Cell Signaling Technology).

Techniques: Western Blot, Infection, Expressing, Produced, Plaque Assay, Quantitative RT-PCR

(A) Infectious viral particles produced in 293FT WT, TMEM41B KO clone #1, and VMP1 KO clone #1 cells upon FA supplementation. DENV1 infection was carried out at MOI of 1 and progeny viruses were harvested at 72 hours post-infection. HCoV-OC43 infection was done at MOI of 0.5 and progeny viruses were harvested at 48 hours post-infection. Cells were treated with a 1:1 mixture of oleic and linoleic acid (FA), or BSA. (B) Confocal microscopy imaging of WT and TMEM41B KO clone #1 cells infected with DENV1 and supplemented with FAs or BSA; dsRNA is stained in red and cell nuclei are displayed in blue. Image scales are indicated in the merged images. (C) RT-qPCR analysis of DENV RNA levels in infected (MOI of 1, 48 hours post-infection) WT and KO cells, upon treatment with FA or BSA. Values demonstrated are relative to DENV RNA load in infected WT cells. 18S rRNA has been used as the internal control. (D) Western blotting analysis to compare DENV viral protein accumulation in WT and TMEM41B KO cells, with and without FA supplementation. Cells were infected with DENV1 at MOI of 1 and lysates were harvested 48 hours post-infection. GAPDH was used as a loading control. (E) TEM imaging of DENV1-infected (MOI of 5, 48 hours post-infection) 293FT WT and TMEM41B KO clone #1. Virion-like particles and classical DENV ROs are indicated with blue and red arrowheads, respectively. Nuclei are identified by “N”. The ER structures are indicated as “ER”. Scale bars are as indicated. All data shown represent results from at least two independent experiments. Error bars represent mean +/- SEM, n = 3. * indicates p-value < 0.05 as determined by two-tailed unpaired t-test or one-way ANOVA. LOD indicates the limit of detection.

Journal: PLoS Pathogens

Article Title: TMEM41B and VMP1 modulate cellular lipid and energy metabolism for facilitating dengue virus infection

doi: 10.1371/journal.ppat.1010763

Figure Lengend Snippet: (A) Infectious viral particles produced in 293FT WT, TMEM41B KO clone #1, and VMP1 KO clone #1 cells upon FA supplementation. DENV1 infection was carried out at MOI of 1 and progeny viruses were harvested at 72 hours post-infection. HCoV-OC43 infection was done at MOI of 0.5 and progeny viruses were harvested at 48 hours post-infection. Cells were treated with a 1:1 mixture of oleic and linoleic acid (FA), or BSA. (B) Confocal microscopy imaging of WT and TMEM41B KO clone #1 cells infected with DENV1 and supplemented with FAs or BSA; dsRNA is stained in red and cell nuclei are displayed in blue. Image scales are indicated in the merged images. (C) RT-qPCR analysis of DENV RNA levels in infected (MOI of 1, 48 hours post-infection) WT and KO cells, upon treatment with FA or BSA. Values demonstrated are relative to DENV RNA load in infected WT cells. 18S rRNA has been used as the internal control. (D) Western blotting analysis to compare DENV viral protein accumulation in WT and TMEM41B KO cells, with and without FA supplementation. Cells were infected with DENV1 at MOI of 1 and lysates were harvested 48 hours post-infection. GAPDH was used as a loading control. (E) TEM imaging of DENV1-infected (MOI of 5, 48 hours post-infection) 293FT WT and TMEM41B KO clone #1. Virion-like particles and classical DENV ROs are indicated with blue and red arrowheads, respectively. Nuclei are identified by “N”. The ER structures are indicated as “ER”. Scale bars are as indicated. All data shown represent results from at least two independent experiments. Error bars represent mean +/- SEM, n = 3. * indicates p-value < 0.05 as determined by two-tailed unpaired t-test or one-way ANOVA. LOD indicates the limit of detection.

Article Snippet: The following primary antibodies were used to detect the presence of indicated proteins in this study: TMEM41B (HPA014946; 1:700; Sigma-Aldrich), VMP1 (12978S; 1:1000; Cell Signaling Technology), GAPDH (GTX627408; 1:5000; GeneTex) or (60004-1-Ig; 1:10000; Proteintech), DENV NS3 (GTX124252; 1:3000; GeneTex), HCoV-OC43 N (MAB9012; 1:3000; Merck), RIG-I (3743S; 1:1000; Cell Signaling Technology), MDA5 (5321S; 1:1000; Cell Signaling Technology), TBK1 (3504S; 1:1000; Cell Signaling Technology), and p-TBK1 (5483S; 1:1000; Cell Signaling Technology).

Techniques: Produced, Infection, Confocal Microscopy, Imaging, Staining, Quantitative RT-PCR, Western Blot, Two Tailed Test