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All (2)
New England Biolabs
Promega
 

About 2 products / 1,012 snippets / 70 images

98 / 100
Bioz Stars
Lambda DNA
New England Biolabs  
Catalog#: N3011
Techniques
 
Article Snippets
"okyo, Japan) to keep the buffer cool during electrophoresis. After electrophoresis, the DNA in the gel was stained for 30 minutes in GelRed (Biotium, Hayward, CA) solution made in TAE buffer. Lambda DNA-HindIII digest marker (New England Biolabs) photographs .."
98 / 100
Bioz Stars
Lambda DNA
New England Biolabs  
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Catalog#: N3011
Techniques
 
Article Snippets
"okyo, Japan) to keep the buffer cool during electrophoresis. After electrophoresis, the DNA in the gel was stained for 30 minutes in GelRed (Biotium, Hayward, CA) solution made in TAE buffer. Lambda DNA-HindIII digest marker (New England Biolabs) photographs .."
86 / 100
Bioz Stars
N3011 HaloTag Succinimidyl Ester (O4) Ligand Promega
Promega  
star_border
Techniques
 
Article Snippets
".. Cat# S6650 Lambda phage DNA New England Biolabs Cat# N3011 HaloTag Succinimidyl Ester (O4) Ligand Promega Cat# P6751 Sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate ThermoFisher Cat# A39268 5% .."  (see more)
66+  more images
Global QAOS. (A) Outline of global QAOS. A random tagging primer t(n) x, containing a tag t, and a random nucleic acid-binding sequence with x random nucleotides, anneals to ssDNA. The first round of primer extension creates a library of molecule s. The mixture is then denatured, and cooled to allow the primer t(n)x to anneal to the library of molecule s. A second round of primer extension creates a library of molecules u. The library u is amplified by stringent PCR using the primer t. (B) Detection of ssDNA-dependent PCR products. Samples containing 0, 5 and 0.5% ssDNA were mixed with random tagging primers containing 6 = t(n)6, 9 = t(n)9 or 12 = t(n)12 random nucleotides at the 3′ end. A no template control (NTC) and no first round primer controls were also subject to global QAOS. After PCR the samples were analysed by agarose gel electrophoresis, along with molecular weight markers (MWM). The sizes of the major molecular weight markers are indicated on the left in kilobases. (C) Key of agarose gel examined in (D–F). The same set of samples described in (B), and a 4-fold dilution series of <t>sonicated</t> <t>DNA</t> standards were separated on an agarose gel. The λ standard series contained sonicated <t>λ</t> <t>DNA</t> (the dsDNA initially present in all tubes). The plasmid standard series contained sonicated, double-stranded, plasmid DNA (that had been present, in single stranded form, in the 5 and 0.5% ssDNA samples). (D) Agarose gel stained with ethidium bromide after electrophoresis. The samples are as described in (C). The low molecular weight bands present in the top two rows are PCR primers. The bands visible above the primers are the ssDNA-dependent PCR product. (E) Southern blot hybridised to λ DNA (dsDNA) probe. The gel was blotted on to a nylon membrane and hybridised with labelled λ DNA. (F)Southern blot hybridised to plasmid DNA (ssDNA) probe. The gel in (D) was blotted on to a nylon membrane and hybrised with labelled plasmid DNA.
BIND-A
SEQ
AMP
GE-A
MOLEC-W
(A) Possible configurations of sister replisomes. The sister replisomes assembled at each origin (i) travel away from each other (ii) or remain physically coupled (iii). Doubly-tethered DNA is replicated efficiently by independently functioning replisomes (iv) but not physically coupled replisomes, which stall after available slack in the DNA is consumed (v). (B) Protocol to induce replication <t>of</t> <t>λ</t> <t>DNA</t> immobilized at one end in a flow cell. (C) Visualization of replicated DNA by TIRF microscopy. λ DNA was incubated with extracts lacking (i, ii) or containing (iii, iv) Geminin and stained with anti-digoxigenin or SYTOX, as indicated.
MICRO
INCU
STAIN
Complementation in KUN virus replicon-expressing BHK (repBHK) cells of full-length KUN virus RNA with a GDD-to-GVD mutation in the NS5 gene. (A) Northern blot analysis of total RNA isolated from repBHK cells (lanes 2 and 4) or normal BHK cells (lanes 3 and 5) at 3 days after transfection with FLGVD RNA (p0) and at 2 days after infection with 3-day-posttransfection culture fluid (p1), with a radiolabelled cDNA probe representing 977 nucleotides of KUN virus prM and E genes (see Materials and Methods). Lane 1 contains mock-transfected repBHK cells, and lane 6 contains 10 ng of control FLGVD RNA transcribed in vitro. An arrowhead indicates the position of RNA of about 11 kb, determined relative to migration in the same gels of ethidium <t>bromide-stained</t> <t>λ</t> <t>DNA</t> digested with BstEII (New England Biolabs). (B) RT-PCR analysis with F1 and R1 primers (Fig. ​(Fig.3A)3A) of total RNA isolated from FLGVD RNA-transfected normal BHK cells. Lane 1 contains λ DNA digested with BstEII (New England Biolabs). Lanes 2 and 3 contain RNA samples isolated from normal BHK cells at 3 and 6 days (3d and 6d), respectively, after electroporation with FLGVD RNA. Lane 4 contains the control DNA fragment of 2.5 kb obtained by PCR amplification of FLGVD cDNA. (C) Comparison of the nucleotide and deduced amino acid (boldface italic letters above the nucleotides) sequences in the GDD motif of wild-type (wt) and GVD mutated cDNAs with that of revertant (rev) cDNA. The sequence of the rev cDNA was obtained by automatic cycle sequencing of the purified 2.5-kb RT-PCR fragment shown in lane 3 in panel B with appropriate primers and by using an ABI PRISM Dye Terminator Cycle Sequencing Ready Reaction Kit (Perkin-Elmer, Brisbane, Australia). Boldface underlined letters represent nucleotides that either were targeted for mutation (AT in the wt sequence), mutated (TC in the GVD sequence), or reverted after replication of FLGVD RNA in BHK cells (AC in the rev sequence). Boxed letters show the SalI recognition site introduced by the GVD mutation.
EXP
MUTA
NORTH-BLOT
ISOLAT
TNSF
Methylation by CcrM MTase alters the sensitivity of phage <t>lambda</t> <t>DNA</t> to REases BfrBI and NsiI. Methylation and the cleavage assay were performed as described in Materials and Methods. The REases used are indicated at the top. Phage lambda DNA was (+) or was not (−) methylated with CcrM MTase and incubated with REases.
METHY
LAMBDA-DNA
CLEV-A
INCU
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