Journal: Cancer Communications

Article Title: Intratumoral CD103 + CD8 + T cells predict response to neoadjuvant chemoimmunotherapy in advanced head and neck squamous cell carcinoma

doi: 10.1002/cac2.12480

Figure Lengend Snippet: The increased CD103 + CD8 + TIL density is associated with better therapeutic response in NACI‐treated HNSCC patients. (A) mIHC image of DAPI, Cytokeratin (CK), CD103, and CD8 staining of advanced HNSCC tissues in the responders and non‐responders. Scale bar, 100 μm. (B) Density of intratumoral CD103 + CD8 + TILs and CD103 − CD8 + TILs in tumors was assessed in the MPR group ( n = 22) and IPR group ( n = 17) patients to NACI via mIHC assay. Density of intratumoral CD103 + CD8 + TILs and CD103 − CD8 + TILs in tumors was assessed in the responders (R; n = 19) and non‐responders (NR; n = 20) patients to NACI. Image shows CD103 + CD8 + (yellow), CD103 − CD8 + (red), and CD8 + (red) cells in advance HNSCC (lower panel). Scale bar, 10 μm. (C) MRI showing the tumor size in responders and non‐responders before and after NACI, respectively. (D) mIHC image shows the density of CD103 + CD8 + TILs in tumors from responders and non‐responders before and after NACI. Scale bar, 200 μm. (E) The temporal alterations of the density of CD103 + CD8 + TILs and CD103 − CD8 + TILs from pre‐ and post‐NACI in the responders ( n = 19) and non‐responders ( n = 20) via mIHC assay. (F) Distribution of CD103 + CD8 + TILs between different clinical characteristics in patients with advanced HNSCC. Horizontal lines indicate the mean ± SEM. Significance was determined with a 2‐tailed Mann‐Whitney U test. Pearson's chi‐square test was used for studying the distribution of intraepithelial CD103 + CD8 + TILs across different subgroups. Abbreviations: TIL, tumor‐infiltrating cell; HNSCC, head and neck squamous cell carcinoma; mIHC, multiplex immunofluorescent; MRI, Magnetic resonance imaging; MPR, major pathologic response; IPR, incomplete pathologic response; R, responders; NR, non‐responders; SEM, standard error of the mean; NACI, neoadjuvant chemoimmunotherapy; MRI, magnetic resonance imaging.

Article Snippet: The following antibodies were purchased from Cell Signaling Technology (CST, Danvers, MA, USA) or Abcam (Waltham, MA, USA): CD8 (1:200, #85336, CST), CD103 (1:200, EPR22590‐27, Abcam), cytokeratin (1:250, #67306, CST), CD68 (1:1000, #76437, CST), and TREM2 (1:500, #91068, CST).

Techniques: Clinical Proteomics, Staining, MANN-WHITNEY, Multiplex Assay, Magnetic Resonance Imaging

Journal: Cancer Communications

Article Title: Intratumoral CD103 + CD8 + T cells predict response to neoadjuvant chemoimmunotherapy in advanced head and neck squamous cell carcinoma

doi: 10.1002/cac2.12480

Figure Lengend Snippet: Intraepithelial CD103 + CD8 + TIL density has a strong predictive value for therapeutic response in HNSCC patients treated with NACI. (A) Overview of the automated image analysis pipeline via inform analysis (upper panel). The spatial distribution of CD103 + CD8 + TILs in the responders and non‐responders (lower panel). Digital markup image shows tumor (red) and stromal (green) zones of the tumor section defined by cytokeratin staining. Digital markup image shows CD103 + CD8 + TILs (red). Scale bar, 100 μm. (B) Density of intraepithelial and stromal CD103 + CD8 + TILs in tumors were assessed in the MPR group ( n = 22) and IPR group ( n = 17) patients to NACI via inform analysis. Density of intraepithelial and stromal CD103 + CD8 + TILs in tumors were assessed in the responders (R; n = 19) and non‐responders (NR; n = 20) with NACI. (C) HALO® spatial analyses demonstrate the spatial distribution of CD103 + CD8 + TILs in the responders and non‐responders. Digital markup image shows tumor (red) and stromal (green) zones of the tumor section defined by cytokeratin staining. Digital markup image shows the density of intratumoral CD103 + CD8 + TILs (blue), intraepithelial CD103 + CD8 + TILs (red), and stromal CD103 + CD8 + TILs (green). Scale bar, 1 mm, 200 μm. Proximity analysis histograms of CD103 + CD8 + TILs within an 800 μm radius by progressive segments of 200 μm bands in advanced HNSCC tissues. (D) Temporal alterations in the density of intraepithelial CD103 + CD8 + TILs from pre‐ and post‐NACI in the responders ( n = 19) and non‐responders via inform analysis ( n = 20). (E) Predictive value of intratumoral CD103 + CD8 + TILs, intraepithelial CD103 + CD8 + TILs, and stromal CD103 + CD8 + TILs via ROC analysis. (F) Distribution of intraepithelial CD103 + CD8 + TILs between different clinical characteristics in patients with advanced HNSCC. Horizontal lines indicate the mean ± SEM. Significance was determined with a 2‐tailed Mann‐Whitney U test. Pearson's chi‐square test was used for studying the distribution of intraepithelial CD103 + CD8 + TILs across different subgroups. Abbreviations: TIL, tumor‐infiltrating cell; HNSCC, head and neck squamous cell carcinoma; MPR, major pathologic response; IPR, incomplete pathologic response; ROC, Receiver Operating Characteristic; R, responders; NR, non‐responders; SEM, standard error of the mean; NACI, neoadjuvant chemoimmunotherapy.

Article Snippet: The following antibodies were purchased from Cell Signaling Technology (CST, Danvers, MA, USA) or Abcam (Waltham, MA, USA): CD8 (1:200, #85336, CST), CD103 (1:200, EPR22590‐27, Abcam), cytokeratin (1:250, #67306, CST), CD68 (1:1000, #76437, CST), and TREM2 (1:500, #91068, CST).

Techniques: Clinical Proteomics, Staining, MANN-WHITNEY

Journal: Differentiation; research in biological diversity

Article Title: Development of the human prepuce and its innervation

doi: 10.1016/j.diff.2019.10.002

Figure Lengend Snippet: Antibodies used in this study

Article Snippet: Intervening sections were processed for immunohistochemistry using the following primary antibodies ( ). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Antibody Source Catalogue # Concentration E-cadherin BD Transduction Laboratories 610181 1/100 Keratin 6 Acris Antibodies AM21068PU-S 1/200 Keratin 10 BioGenex LL002 1/100 Keratin 14 E.B.

Techniques: Concentration Assay, Transduction