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Bio-Rad

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About 26 products / 57 snippets / 11 images

	96 / 100 Bioz Stars		StrepMAB-Classic IBA Lifesciences Sponsored			Visit Supplier
			Catalog#: 2-1507-001 Techniques Article Snippets ") at 4 °C. SFV Env ectodomains were added to the cell pellet for 1 h. Cells were washed twice, incubated with StrepMAB-Classic-HRP antibody that recognizes the strep tag at the C-terminus of the SFV Env ectodomain (7.5 µg/ml, IBA Lifesciences #2-1509-001) .."	× MORE IMAGES FROM THIS ARTICLE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query StrepMAB-Classic IBA Lifesciences Article Snippet "" Figure Legend " " MORE IMAGES FROM THIS ARTICLE

	94 / 100 Bioz Stars		Classical Complement Pathway, Mouse, Assay Hycult Biotech Sponsored			Visit Supplier
			Catalog#: HIT420 Techniques Article Snippets "veraged. Serum samples collected 24 h after reperfusion were assessed for functional complement pathway activities using the following ELISA kits from Hycult Biotech (Uden, The Netherlands): mouse classical (HIT420), lectin (HIT421), and alternative pathway (HIT422). Plasma samples were analysed for " (see more)	× MORE IMAGES FROM THIS ARTICLE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Classical Complement Pathway, Mouse, Assay Hycult Biotech Article Snippet "" Figure Legend " " MORE IMAGES FROM THIS ARTICLE

	96 / 100 Bioz Stars		StrepMAB-Classic IBA Lifesciences		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Catalog#: 2-1507-001 Techniques Article Snippets ") at 4 °C. SFV Env ectodomains were added to the cell pellet for 1 h. Cells were washed twice, incubated with StrepMAB-Classic-HRP antibody that recognizes the strep tag at the C-terminus of the SFV Env ectodomain (7.5 µg/ml, IBA Lifesciences #2-1509-001) .."	× MORE IMAGES FROM THIS ARTICLE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query StrepMAB-Classic IBA Lifesciences Article Snippet "" Figure Legend " " MORE IMAGES FROM THIS ARTICLE

	93 / 100 Bioz Stars		Mouse anti Strep-Tag Classic Bio-Rad		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Catalog#: MCA2489P Techniques Strep-tag, Western Blot, Staining, Incubation, Purification (see more) Article Snippets "The HA expression was analysed with SDS-PAGE followed by Western-blot on PVDF membrane (Biorad) using α-strep-tag mouse antibodies 1:3000 (IBA Life Sciences)." (see more)	× MORE IMAGES FROM THIS ARTICLE Fluorescent trimeric hemagglutinins reveal multivalent receptor binding properties Journal of molecular biology Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Fluorescent trimeric hemagglutinins reveal multivalent receptor binding properties Journal of molecular biology 2000 Jan 01 Product Images (3) Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Mouse anti Strep-Tag Classic Bio-Rad Article Snippet "The HA expression was analysed with SDS-PAGE followed by Western-blot on PVDF membrane (Biorad) using α-strep-tag mouse antibodies 1:3000 (IBA Life Sciences)." Figure Legend "Figure 4. (A) PR8 D190 without precomplexing (B) PR8D190E without precomplexing (C) PR8 D190 precomplexed with α-strep-HRP and α-mouse-647 (D) PR8 D190E precomplexed with α-strep-HRP and α-mouse-647 (E) PR8 CAM precomplexed with α-Strep-488 and α-Mouse-488 (F) PR8 D190E precomplexed with α-Strep-488 and α-Mouse-488. The mean signal and standard error were calculated from six independent replicates of glycans printed on the array. The data shown is a representative of three independent assays. α2–3 linked sialosides are shown in white bars (glycans 11 to 77 on the x axis) and α2–6 linked sialosides in black (glycans 78 to 130). Glycans 1 to 10 are non-sialylated controls (see also Table S1) " MORE IMAGES FROM THIS ARTICLE Journal of molecular biology Fluorescent trimeric hemagglutinins reveal multivalent receptor binding properties

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(A) PR8 D190 without precomplexing (B) PR8D190E without precomplexing (C) PR8 D190 precomplexed with <t>α-strep-HRP</t> and α-mouse-647 (D) PR8 D190E precomplexed with α-strep-HRP and α-mouse-647 (E) PR8 CAM precomplexed with α-Strep-488 and α-Mouse-488 (F) PR8 D190E precomplexed with α-Strep-488 and α-Mouse-488. The mean signal and standard error were calculated from six independent replicates of glycans printed on the array. The data shown is a representative of three independent assays. α2–3 linked sialosides are shown in white bars (glycans 11 to 77 on the x axis) and α2–6 linked sialosides in black (glycans 78 to 130). Glycans 1 to 10 are non-sialylated controls (see also Table S1)

BMDMs were infected with amastigotes at a MOI of 4:1. (A) Western blot analysis of <t>mature</t> <t>IL-1β</t> and caspase-1 p20 in culture supernatants (SN), and pro-caspase-1 and pro-IL-1β in macrophage lysates (day 3 PI). (B) Western blot analysis of ASC in cell lysates and pellets (day 3 PI). (C) Western blot analysis of NLRP3, NLRC4, AIM-2 and RIG-I inflammasomes in BMDM lysates (day 3 PI). Beta-actin was used as a control protein. (D) Transcriptional modulation of inflammasome components as assessed by RT-qPCR. BMDMs were loaded with live ( L. am ) or heat-killed (HK L. am ) amastigotes, or latex beads and cultured for 3 days. As positive control, NLRP3 activation was induced after LPS and ATP stimulation. The expression fold changes (FC) are indicated using uninfected and unstimulated macrophages as a calibrator. Histograms display mean FC values ± SEM for L. am -infected and LPS + ATP-treated uninfected BMDMs (n = 4-13 independent experiments) and for HK L. am and latex beads-loaded BMDMs (technical duplicates, one representative experiment is shown). (E, F) Biparametric dot plots displaying transcript modulation (fold changes, FC) of IL-1β (E) and IL-18 (F) and parasite load (mean number of amastigotes per macrophage) at different time points PI (indicated by the numeric label of the data points). The F-test p-value is indicated for the corresponding linear regression. (G) Western Blot analyses of inflammasome components at day 3 PI in culture supernatants (SN) and lysates from LPS-stimulated and non-stimulated samples. (H, I) Detection by <t>ELISA</t> of secreted IL-1β (H) and IL-18 (I) at day 1 and 3 PI. Blue and red bars represent cytokine levels for uninfected and infected BMDMs, respectively (mean +/− SEM, n = 3-6). nd, not detected; *, p < 0.06; **, p < 0.05.

INFECT

WB

qRT-PCR

CELL-CTR

POS-CON

STREP-TAG

FLAG-T

VIR

RECOMB

ELISA

STREP-TAG

VIR

RECOMB

PROT-I

STAIN

	94 / 100 Bioz Stars		Classical Complement Pathway, Mouse, Assay Hycult Biotech		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Catalog#: HIT420 Techniques Article Snippets "veraged. Serum samples collected 24 h after reperfusion were assessed for functional complement pathway activities using the following ELISA kits from Hycult Biotech (Uden, The Netherlands): mouse classical (HIT420), lectin (HIT421), and alternative pathway (HIT422). Plasma samples were analysed for " (see more)	× MORE IMAGES FROM THIS ARTICLE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Classical Complement Pathway, Mouse, Assay Hycult Biotech Article Snippet "" Figure Legend " " MORE IMAGES FROM THIS ARTICLE

	92 / 100 Bioz Stars		Classical mouse strains The Jackson Laboratory		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets "While the classical albino mutation (c), has been propagated in several classical mouse strains, the c-2J mutation arose independently in 1970 at the Jackson Laboratory in the C57BL/6J background." (see more)	× MORE IMAGES FROM THIS ARTICLE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Classical mouse strains The Jackson Laboratory Article Snippet "" Figure Legend " " MORE IMAGES FROM THIS ARTICLE

	91 / 100 Bioz Stars		Classical mouse ELISA kits Thermo Fisher Scientific / eBioscience		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets ".. supernatant using mouse instant ELISA kits (for IL-1β and TNF, eBioscience) or classical mouse ELISA kits for IL-1α (eBioscience) or IL-18 (R&D system Europe)." (see more)	INFECT WB qRT-PCR CELL-CTR POS-CON × MORE IMAGES FROM THIS ARTICLE Leishmania targets the macrophage epigenome and dampens the NF-κB/NLRP3-mediated inflammatory response bioRxiv Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Leishmania targets the macrophage epigenome and dampens the NF-κB/NLRP3-mediated inflammatory response bioRxiv 2000 Jan 01 Product Images (3) Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Classical mouse ELISA kits Thermo Fisher Scientific / eBioscience Article Snippet "Cytokines were quantified in the supernatant using mouse instant ELISA kits (for IL-1β and TNF, eBioscience) or classical mouse ELISA kits for IL-1α (eBioscience) or IL-18 (R&D system Europe)." Figure Legend "Figure 1: BMDMs were infected with amastigotes at a MOI of 4:1. (A) Western blot analysis of mature IL-1β and caspase-1 p20 in culture supernatants (SN), and pro-caspase-1 and pro-IL-1β in macrophage lysates (day 3 PI). (B) Western blot analysis of ASC in cell lysates and pellets (day 3 PI). (C) Western blot analysis of NLRP3, NLRC4, AIM-2 and RIG-I inflammasomes in BMDM lysates (day 3 PI). Beta-actin was used as a control protein. (D) Transcriptional modulation of inflammasome components as assessed by RT-qPCR. BMDMs were loaded with live ( L. am ) or heat-killed (HK L. am ) amastigotes, or latex beads and cultured for 3 days. As positive control, NLRP3 activation was induced after LPS and ATP stimulation. The expression fold changes (FC) are indicated using uninfected and unstimulated macrophages as a calibrator. Histograms display mean FC values ± SEM for L. am -infected and LPS + ATP-treated uninfected BMDMs (n = 4-13 independent experiments) and for HK L. am and latex beads-loaded BMDMs (technical duplicates, one representative experiment is shown). (E, F) Biparametric dot plots displaying transcript modulation (fold changes, FC) of IL-1β (E) and IL-18 (F) and parasite load (mean number of amastigotes per macrophage) at different time points PI (indicated by the numeric label of the data points). The F-test p-value is indicated for the corresponding linear regression. (G) Western Blot analyses of inflammasome components at day 3 PI in culture supernatants (SN) and lysates from LPS-stimulated and non-stimulated samples. (H, I) Detection by ELISA of secreted IL-1β (H) and IL-18 (I) at day 1 and 3 PI. Blue and red bars represent cytokine levels for uninfected and infected BMDMs, respectively (mean +/− SEM, n = 3-6). nd, not detected; , p < 0.06; *, p < 0.05. " INFECT WB qRT-PCR CELL-CTR POS-CON MORE IMAGES FROM THIS ARTICLE bioRxiv Leishmania targets the macrophage epigenome and dampens the NF-κB/NLRP3-mediated inflammatory response

	80 / 100 Bioz Stars		Monoclonal mouse anti-StrepMAB-Classic IBA		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets ".. Tween-20 (PBST), the membrane was incubated for 1 h with a monoclonal mouse anti-StrepMAB-Classic (StrepMAB; iba GmbH) at a 1:3000 dilution." (see more)	× MORE IMAGES FROM THIS ARTICLE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Monoclonal mouse anti-StrepMAB-Classic IBA Article Snippet "" Figure Legend " " MORE IMAGES FROM THIS ARTICLE

	80 / 100 Bioz Stars		Mouse anti Strep-Tag Classic Bio-Rad		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets "Mouse anti Strep-Tag Classic , BioRad , Cat #MCA2489P, RRID: AB_609796." (see more)	STREP-TAG FLAG-T VIR RECOMB ELISA × MORE IMAGES FROM THIS ARTICLE Mosaic sarbecovirus nanoparticles elicit cross-reactive responses in pre-vaccinated animals Cell Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Mosaic sarbecovirus nanoparticles elicit cross-reactive responses in pre-vaccinated animals Cell 2000 Jan 01 Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Mouse anti Strep-Tag Classic Bio-Rad Article Snippet "Mouse anti Strep-Tag Classic , BioRad , Cat #MCA2489P, RRID: AB_609796." Figure Legend " " STREP-TAG FLAG-T VIR RECOMB ELISA MORE IMAGES FROM THIS ARTICLE Cell Mosaic sarbecovirus nanoparticles elicit cross-reactive responses in pre-vaccinated animals

	91 / 100 Bioz Stars		Strep-tag classic mouse monoclonal antibody Bio-Rad		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets ".. beta-tubulin rabbit polyclonal antibody (LI-COR) at 1:1000 and a Strep-tag classic mouse monoclonal antibody (Bio-Rad Cat# MCA2489) at 1:500 dilution, followed by incubation with IRDye .." (see more)	STREP-TAG VIR RECOMB PROT-I STAIN × MORE IMAGES FROM THIS ARTICLE Structural and functional analysis of the Nipah virus polymerase complex Cell Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Structural and functional analysis of the Nipah virus polymerase complex Cell 2000 Jan 01 Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Strep-tag classic mouse monoclonal antibody Bio-Rad Article Snippet "Proteins were transferred to nitrocellulose by Western blotting and the blots were probed with beta-tubulin rabbit polyclonal antibody (LI-COR) at 1:1000 and a Strep-tag classic mouse monoclonal antibody (Bio-Rad Cat# MCA2489) at 1:500 dilution, followed by incubation with IRDye 680RD-conjugated donkey anti-rabbit (LI-COR Cat# 926-68073) and IRDye 800CW-conjugated goat anti-mouse (LI-COR Cat# 926-32210) antibodies, each at 1:20,000 dilution." Figure Legend " " STREP-TAG VIR RECOMB PROT-I STAIN MORE IMAGES FROM THIS ARTICLE Cell Structural and functional analysis of the Nipah virus polymerase complex

	80 / 100 Bioz Stars		Strep-tag classic mouse monoclonal antibody Bio-Rad		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets ".. with beta-tubulin rabbit polyclonal antibody (LI-COR) at 1:1000 and a Strep-tag classic mouse monoclonal antibody (Biorad) at 1:500." (see more)	× MORE IMAGES FROM THIS ARTICLE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Strep-tag classic mouse monoclonal antibody Bio-Rad Article Snippet "" Figure Legend " " MORE IMAGES FROM THIS ARTICLE

	80 / 100 Bioz Stars		Monoclonal Strep-tag II antibody Bio-Rad		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets "For visualizing the co-purification, Western blot analyses were performed employing a monoclonal Strep-tag II antibody (IBA) at a dilution of 1∶20000 and a secondary anti-mouse horseradish peroxidase labelled goat antibody (BioRad, Germany) at a dilution of 1∶20000." (see more)	RECOMB EXP STREP-TAG PURI AC × MORE IMAGES FROM THIS ARTICLE The Assembly of the Plasmodial PLP Synthase Complex Follows a Defined Course PLoS ONE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us The Assembly of the Plasmodial PLP Synthase Complex Follows a Defined Course PLoS ONE 2000 Jan 01 Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Monoclonal Strep-tag II antibody Bio-Rad Article Snippet "For visualizing the co-purification, Western blot analyses were performed employing a monoclonal Strep-tag II antibody (IBA) at a dilution of 1∶20000 and a secondary anti-mouse horseradish peroxidase labelled goat antibody (BioRad, Germany) at a dilution of 1∶20000." Figure Legend "Figure 4 Cell homogenates of the recombinant expression of the plasmodial Pdx1 protein and Pdx2HIS protein (6× His-tag instead of a Strep-tag) were mixed and subsequently purified via Strep-tag affinity chromatography of the Pf Pdx1 protein. The co-purification was visualized by Western blot analysis using a monoclonal anti-Strep antibody (IBA) and the HIS-probe-HRP (Pierce) as described in . (A) Co-purification of the Pf Pdx1 wild-type (3) and the Pf Pdx1 DKK (D26A, K83A and K151A) triple mutant protein (4) with the Pf Pdx2HIS wild-type. As a control the Pf Pdx1 wild-type (WT) (1) and the Pf Pdx2HIS (2) single proteins were purified via Strep-tag affinity chromatography and applied in Western blot analysis. (B) Co-purification of the Pf Pdx1 ERR (5), Pf Pdx1 RHE (6) as well as the Pf Pdx1 H88A (7) and Pf Pdx1 E91A (8) mutant proteins with the plasmodial Pdx2HIS wild-type. (Note: The Pf Pdx1 R85A mutant was not recombinantly expressible.) (C) The gate-keeper Pf Pdx2HIS E53Y (9) and Pf Pdx2HIS R154W (10) mutants were co-purified with the Pf Pdx1 wild-type protein via its Strep-tag. " RECOMB EXP STREP-TAG PURI AC MORE IMAGES FROM THIS ARTICLE PLoS ONE The Assembly of the Plasmodial PLP Synthase Complex Follows a Defined Course

	91 / 100 Bioz Stars		Anti-mouse HRP-conjugated Bio-Rad		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets ".. 10 min. Primary antibody was detected by a goat anti-rabbit or anti-mouse HRP-conjugated secondary antibodies (Bio-Rad) diluted to 1/2000 in blocking solution." (see more)	× MORE IMAGES FROM THIS ARTICLE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query Anti-mouse HRP-conjugated Bio-Rad Article Snippet "" Figure Legend " " MORE IMAGES FROM THIS ARTICLE

	92 / 100 Bioz Stars		(HRP) Anti mouse Bio-Rad		Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us	Visit Supplier
			Techniques Article Snippets "Horseradish peroxidase-conjugated (HRP) antibodies against mouse (1:5000; Bio-Rad, Hercules, CA, USA) or against rabbit (1:5000; Cell .." (see more)	× MORE IMAGES FROM THIS ARTICLE Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us Open an account FREE Academic Industry already a user? Sign in are you a supplier? Contact us 1x 2x 3x 6x Product associated with above image that matches your search query (HRP) Anti mouse Bio-Rad Article Snippet "" Figure Legend " " MORE IMAGES FROM THIS ARTICLE