Journal: Journal of neurology

Article Title: Rho GTPase-activating protein 10 (ARHGAP10/GRAF2) is a novel autoantibody target in patients with autoimmune encephalitis.

doi: 10.1007/s00415-022-11178-9

Figure Lengend Snippet: Fig. 1 Binding of serum IgG1 (A-D), IgA (E) and IgM (F) from a patient with ACA to both ARHGAP26-transfected (A) and ARHGAP10-transfected HEK293 cells (B, E–F) and to primate cerebellum tissue sections (D) but not to mock- transfected HEK293 cells (C, E inset, F inset). Red (AF568) indicates bound IgG1 in (A-D); green (FITC) indicates bound IgA or IgM in (E–F); blue indi- cates nuclear staining by DAPI

Article Snippet: For the WB, mouse cerebellum lysate (sc-2403; Santa Cruz, TX, USA) was blotted on a Immobilon-P PVDF transfer membrane (0.45 μm) (Merck, Darmstadt, Germany), cut into strips and incubated with patient serum (1:20 dilution in PBS) and polyclonal rabbit antibodies to human ARHGAP26 (PTGLab, Manchester, UK) or ARHGAP10 (PTGLab), respectively.

Techniques: Binding Assay, Transfection, Staining

Journal: Nature methods

Article Title: Significance analysis for clustering with single-cell RNA-sequencing data

doi: 10.1038/s41592-023-01933-9

Figure Lengend Snippet: a, The number of clusters and ARI found under our approach when the size of the fifth population is varied from 50 to 550 cells in the five-populations and five-similar-populations dataset, b, The computational time of sc-SHC on subsets of a mouse cerebellum dataset (brain) ranging from 1,000 to 100,000 cells, and the computational time of our significance analysis on pre-computed clusters for subsets of the Human Lung Cell Atlas (lung), ranging from 1,000 to 65,000 cells.

Article Snippet: The mouse cerebellum atlas is available at the Broad Institute Single Cell Portal with study ID SCP795.

Techniques: