Journal: mBio

Article Title: Signaling between mammalian adiponectin and a mosquito adiponectin receptor reduces Plasmodium transmission

doi: 10.1128/mbio.02257-23

Figure Lengend Snippet: Mammalian adiponectin co-localizes with the mosquito midgut. (A) Following a sugar meal or blood meal, mRNA from the mosquito midgut, ovary, or fat body was collected. The expression level of the adiponectin receptor was determined by RT-PCR. Each dot in the midgut and ovary groups represents a pooled sample from two mosquitoes. Each dot in the fat body group represents a pooled sample from four mosquitoes. (Mean ± S.D.) (B) Murine adiponectin bound to mosquito midguts. His-tag murine adiponectin or control protein (BSA) was incubated with the mosquito midgut, and the presence of adiponectin in the midgut was determined by confocal microscopy.

Article Snippet: The midgut was dissected from the mosquitoes and then incubated with 1 µg/µL of mouse adiponectin (SinoBiological, #50636-M08H), or BSA for 1 hour.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Incubation, Confocal Microscopy

Journal: mBio

Article Title: Signaling between mammalian adiponectin and a mosquito adiponectin receptor reduces Plasmodium transmission

doi: 10.1128/mbio.02257-23

Figure Lengend Snippet: Lack of adiponectin in the incoming blood meal increases infection of Anopheles gambiae with Plasmodium berghei . (A) P. berghei -infected RBCs were collected from Adipoq -/- mice, and then equal amounts of plasma from Adipoq -/- mice or wild-type mice were mixed with the infected RBCs. Mosquitoes were fed on blood lacking adiponectin or wild-type plasma by membrane feeding. Two days after the blood meal, the midguts were collected, and the expression levels of the adiponectin receptor ( A ) and the burden of Plasmodium were determined by RT-qPCR ( B ). (C) Fourteen days after the blood meal, the midguts of mosquitoes were collected and the burden of Plasmodium was determined by RT-qPCR. (D) Mosquitoes were injected with dsAdpR ( AdpR knockdown, AdpR KD), or dsGluc (control). Two days after ds RNA injection, the mosquitoes were fed on P. berghei -infected mice. Two days after the blood meal, the midguts were collected and the expression levels of adiponectin receptor ( D ) and the burden of Plasmodium were determined by RT-PCR ( E ). (F) Fourteen days after the blood meal, the midguts of mosquitoes were collected, and the burden of Plasmodium was determined by RT-PCR. Each dot represents one mosquito. (Median ± interquartile range, P < 0.05 using the Mann-Whitney U-test.)

Article Snippet: The midgut was dissected from the mosquitoes and then incubated with 1 µg/µL of mouse adiponectin (SinoBiological, #50636-M08H), or BSA for 1 hour.

Techniques: Infection, Membrane, Expressing, Quantitative RT-PCR, Injection, Reverse Transcription Polymerase Chain Reaction, MANN-WHITNEY

Journal: mBio

Article Title: Signaling between mammalian adiponectin and a mosquito adiponectin receptor reduces Plasmodium transmission

doi: 10.1128/mbio.02257-23

Figure Lengend Snippet: Gene expression analysis reveals that lipophorin (AGAP001826) is regulated by the adiponectin receptor. Mosquitoes were injected with dsAdpR ( AdpR knockdown, KD) or dsGluc (control, Ctrl). Two days after ds RNA injection, the mosquitoes were fed on P. berghei -infected mice. Two days after the blood meal, each midgut was collected, and RNA from each midgut was isolated for RNA-seq analysis. (A) A hierarchical clustering of differentially expressed genes was generated by comparing data from AdpR knockdown and control mosquitoes. (B) Volcano plots showing significant changes in gene expression between AdpR knockdown and control mosquitoes. Dispersion graph of –10*log10( P value) (y-axis) against the log2(fold change) (x-axis) corresponding to the genes by their differential expression. (C) RT-qPCR validation of gene expression in the mosquito midgut after injection with ds AdpR (Adiponectin knockdown, AdpR KD) or ds Gluc (control). Two days after ds RNA injection, mosquitoes were fed on P. berghei -infected mice. Two days after the blood meal, the midguts were collected for RT-qPCR. (D) RT-qPCR validation of expression in mosquito midguts 2 days after feeding on adiponectin knockout (Adipoq -/- ) or wild-type P. berghei -infected mice. Each dot represents one mosquito. (Median ± interquartile range, P < 0.05 using the Mann-Whitney U-test.)

Article Snippet: The midgut was dissected from the mosquitoes and then incubated with 1 µg/µL of mouse adiponectin (SinoBiological, #50636-M08H), or BSA for 1 hour.

Techniques: Expressing, Injection, Infection, Isolation, RNA Sequencing Assay, Generated, Dispersion, Quantitative RT-PCR, Knock-Out, MANN-WHITNEY

Journal: mBio

Article Title: Signaling between mammalian adiponectin and a mosquito adiponectin receptor reduces Plasmodium transmission

doi: 10.1128/mbio.02257-23

Figure Lengend Snippet: Adiponectin signaling affects Plasmodium infection in mosquitoes through the lipophorin pathway. Mosquitoes were injected with ds AdpR ( AdpR knockdown), ds Lp ( lipophorin knockdown), both ds AdpR and ds Lp, or dsGluc (control). Two days after injection, the mosquitoes were fed on P. berghei -infected mice. Two days after the blood meal, the midguts were collected, and the expression levels of adiponectin receptor ( A ) and lipophorin ( B ) were determined by RT-qPCR. (C) Hemolymph from 10 knockdown or control mosquitoes was collected, and the levels of lipophorin (Lp) and vitellogenin (Vg) were determined by SDS-PAGE gel. (D) The Plasmodium burden was determined by RT-qPCR. Each dot represents one mosquito. (Median ± interquartile range, P < 0.05 using the Mann-Whitney U-test.)

Article Snippet: The midgut was dissected from the mosquitoes and then incubated with 1 µg/µL of mouse adiponectin (SinoBiological, #50636-M08H), or BSA for 1 hour.

Techniques: Infection, Injection, Expressing, Quantitative RT-PCR, SDS Page, MANN-WHITNEY

Journal: mBio

Article Title: Signaling between mammalian adiponectin and a mosquito adiponectin receptor reduces Plasmodium transmission

doi: 10.1128/mbio.02257-23

Figure Lengend Snippet: Silencing of adiponectin signaling affects the oocyst number and size in mosquitoes. Mosquitoes were injected with ds AdpR ( AdpR knockdown) or dsGluc (control). Two days after injection, the mosquitoes were fed on GFP-expressing P. berghei -infected mice. (A) Seven days after the blood meal, the midguts were collected. The number of oocysts per midgut was determined by the GFP signals using EVOS fluorescence microscopy. The dot blots represent the number of oocysts from each group of infected midguts. (Median ± interquartile range (IQR), P < 0.05 using the Mann-Whitney U-test.) The pie charts represent the percentage of infected mosquitoes in each group ( P < 0.05 using the Chi-square test). (B) The size of oocysts in each group was determined by GFP signals using EVOS fluorescence microscopy. (Median ± IQR, P < 0.05 using the Mann-Whitney U-test.)

Article Snippet: The midgut was dissected from the mosquitoes and then incubated with 1 µg/µL of mouse adiponectin (SinoBiological, #50636-M08H), or BSA for 1 hour.

Techniques: Injection, Expressing, Infection, Fluorescence, Microscopy, MANN-WHITNEY

Journal: Journal of Molecular Neuroscience

Article Title: Unraveling Molecular Mechanisms of THAP1 Missense Mutations in DYT6 Dystonia

doi: 10.1007/s12031-020-01490-2

Figure Lengend Snippet: THAP1 molecular weight and subcellular distribution of THAP1. a Western blot showing a 30-kDa band and a 25-kDa band in HEK cells overexpressing wild-type THAP1 or F21L mutant THAP1 using the Proteintech antibody (12584-1-AP). A small band (around 27 kDa) was observed in HEK cells overexpressing Q154fs180X mutant THAP1. Mass spectrometry analysis detected two THAP1 peptides in the 30-kDa band (peptide 1, IHQLEQQVEK; peptide 2, LKEVVHFQK) but not in the 25-kDa band. b Wild-type and missense mutant (F81L and L180S) THAP1 protein distributed mainly in the nuclear fraction. Lamin A/C was used as marker of nuclear protein

Article Snippet: Fig. 1 THAP1 molecular weight and subcellular distribution of THAP1. a Western blot showing a 30-kDa band and a 25-kDa band in HEK cells overexpressing wild-type THAP1 or F21L mutant THAP1 using the Proteintech antibody (12584-1-AP).

Techniques: Molecular Weight, Western Blot, Mutagenesis, Mass Spectrometry, Marker

Journal: International Journal of Molecular Sciences

Article Title: Supervised Clustering of Adipokines and Hormonal Receptors Predict Prognosis in a Population of Obese Women with Type 1 Endometrial Cancer

doi: 10.3390/ijms18051055

Figure Lengend Snippet: Examples of immunostaining: the positive staining was brown for each antibody; ( A ) PR immunostaining (×10); ( B ) ER immunostaining (10×); ( C ) SPARC immunostaining (10×); ( D ) stromal SPARC immunostaining (×10); ( E ) stromal adiponectin immunostaining (10×); ( F ) stromal IL-6 immunostaining (×20); ( G ) RBP4 immunostaining (10×); ( H ) TNF α immunostaining (10×).

Article Snippet: We used a mouse monoclonal antibodies for adiponectin (1/100, 1B2, TA503801, Origene ® , Rockville, MD, USA), RBP4 (1/100, 4D9, LS-B6142-50, LifeSpan Bioscience ® , Seattle, WA, USA), IL-6 (1/100, ab9324, Abcam ® , Cambridge, UK), TNF α (1/100, 6C10, LS-B7086-50, LifeSpan Bioscience ® , Seattle, WA, USA).

Techniques: Immunostaining, Staining

Journal: International Journal of Molecular Sciences

Article Title: Supervised Clustering of Adipokines and Hormonal Receptors Predict Prognosis in a Population of Obese Women with Type 1 Endometrial Cancer

doi: 10.3390/ijms18051055

Figure Lengend Snippet: Protein clustering in recurrence vs. non recurrence groups in obese population.

Article Snippet: We used a mouse monoclonal antibodies for adiponectin (1/100, 1B2, TA503801, Origene ® , Rockville, MD, USA), RBP4 (1/100, 4D9, LS-B6142-50, LifeSpan Bioscience ® , Seattle, WA, USA), IL-6 (1/100, ab9324, Abcam ® , Cambridge, UK), TNF α (1/100, 6C10, LS-B7086-50, LifeSpan Bioscience ® , Seattle, WA, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Supervised Clustering of Adipokines and Hormonal Receptors Predict Prognosis in a Population of Obese Women with Type 1 Endometrial Cancer

doi: 10.3390/ijms18051055

Figure Lengend Snippet: Protein clustering in high risk and low risk stage I groups in obese population.

Article Snippet: We used a mouse monoclonal antibodies for adiponectin (1/100, 1B2, TA503801, Origene ® , Rockville, MD, USA), RBP4 (1/100, 4D9, LS-B6142-50, LifeSpan Bioscience ® , Seattle, WA, USA), IL-6 (1/100, ab9324, Abcam ® , Cambridge, UK), TNF α (1/100, 6C10, LS-B7086-50, LifeSpan Bioscience ® , Seattle, WA, USA).

Techniques:

Journal: Particle and Fibre Toxicology

Article Title: Programming of mouse obesity by maternal exposure to concentrated ambient fine particles

doi: 10.1186/s12989-017-0201-9

Figure Lengend Snippet: Maternal exposure to CAP decreases adipose leptin expression. a the plasma leptin levels of adult offspring. n Male/FA = 6, n Male/CAP = 7, n Female/FA = 4, and n Female/CAP = 7. * p < 0.05, one way ANOVA. b the plasma adiponectin levels of adult offspring. n Male/FA = 6, n Male/CAP = 7, n Female/FA = 4, and n Female/CAP = 7. * p < 0.05, one way ANOVA. c the mRNA expression levels of offspring epididymal adipose tissues. n Male/FA = 6, n Male/CAP = 7, n Female/FA = 4, and n Female/CAP = 7. * p < 0.05, one way ANOVA. d the representative image of western blot analysis of leptin protein in epididymal adipose tissues. e the quantification of leptin protein in epididymal adipose tissues. n Male/FA = 6, n Male/CAP = 7, n Female/FA = 4, and n Female/CAP = 7. * p < 0.05, one way ANOVA. f the average methylation level of leptin promoter in adipocytes. n Male/FA = 6, n Male/CAP = 7, n Female/FA = 4, and n Female/CAP = 7. * p < 0.05, one way ANOVA

Article Snippet: Plasma insulin (Ultra Sensitive Mouse Insulin ELISA Kit, Crystal Chemical), leptin (RayBio Mouse Leptin ELISA Kit, RayBiotech), and adiponectin (Mouse Adiponectin ELISA Kit, Boster Biological Technology) levels were determined per manufacturer’s instruction.

Techniques: Expressing, Clinical Proteomics, Western Blot, Methylation