Journal: Neurobiology of disease

Article Title: HIV, Tat and Dopaminergic Transmission

doi: 10.1016/j.nbd.2017.04.015

Figure Lengend Snippet: Tat Models Referenced in Review

Article Snippet: TUNEL, immunocytochemistry, co-immunoprecipitation/blotting , nitrate/nitrite assay kit Defines complex mechanism (formation of PSD95-NMDAR-nNOS complex) by which Tat promotes apoptosis in neurons and astrocytes Silvers 2007 Primary Rodent Cell Culture Primary hippocampal or midbrain neuronal culture from Sprague-Dawley rats exposed to Tat 1-72 50 nM Calcein/Ethidium bromide Live/Dead ratio, ICC, Hoechst staining, radiolabeled binding, immunoblotting, D1 receptor blockade attenuates Tat induced cells death in the primary midbrain neuronal culture, but not in the primary hippocampal culture Aksenov 2008 Primary Rodent Cell Culture Primary hippocampal cell culture from 18 day old Sprague Dawley rats treated with Tat 1-72 (10–250nM) ICC, calcein AM, radiometric ligand-binding Validated the importance of dopamine transporter and pscyhostimualnts in Tat toxixicty.

Techniques: Variant Assay, Rnase Protection Assay, Blocking Assay, Sequencing, In Situ Hybridization, Cell Culture, Infection, Cell Counting, Produced, Transfection, Northern Blot, Labeling, Transactivation Assay, Clinical Proteomics, Membrane, Derivative Assay, Plasmid Preparation, Purification, Western Blot, Immunocytochemistry, Gene Expression, In Vitro, Expressing, Pulse Chase, Permeability, Chemotaxis Assay, Ligand Binding Assay, Staining, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Southern Blot, Injection, TUNEL Assay, Transgenic Assay, Binding Assay, Synthesized, Real-time Polymerase Chain Reaction, Flow Cytometry, Circular Dichroism, Incubation, Patch Clamp, Mass Spectrometry, Ab Array, Dot Blot, Silver Staining, Electrophoretic Mobility Shift Assay, Activity Assay, Microinjection, MTT Assay, Multiplex Assay, Activation Assay, SPR Assay, Animal Model, Phospho-proteomics, Immunoprecipitation, Luciferase, Sedimentation, Recombinant, Inhibition, Mutagenesis, Transmission Assay, Electron Microscopy, Immunohistochemistry, Immunodepletion, Control, Knock-Out, Conditioned Place Preference, Microscopy, Residue

Journal: Neurobiology of disease

Article Title: HIV, Tat and Dopaminergic Transmission

doi: 10.1016/j.nbd.2017.04.015

Figure Lengend Snippet: Tat Models Referenced in Review

Article Snippet: Perry , 2010 , Primary Rodent Cell Culture, , Primary midbrain neuronal culture from Spague-Dawley rats and PC12 cells exposed to Tat 1-72 120nM, Transgenic mice tetracycline condition Tat 1-86 (8-mg/kg/day for 7 days) , Radiolabeled uptake, fluorescent uptake, immunoblotting, ICC, fluorescent reporter labeling , Tat activate calpain proteases to increase cell sruface dopamine transporter levels in two model system midbrain culture and PC12-expressing hDAT cells.

Techniques: Variant Assay, Rnase Protection Assay, Blocking Assay, Sequencing, In Situ Hybridization, Cell Culture, Infection, Cell Counting, Produced, Transfection, Northern Blot, Labeling, Transactivation Assay, Clinical Proteomics, Membrane, Derivative Assay, Plasmid Preparation, Purification, Western Blot, Immunocytochemistry, Gene Expression, In Vitro, Expressing, Pulse Chase, Permeability, Chemotaxis Assay, Ligand Binding Assay, Staining, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Southern Blot, Injection, TUNEL Assay, Transgenic Assay, Binding Assay, Synthesized, Real-time Polymerase Chain Reaction, Flow Cytometry, Circular Dichroism, Incubation, Patch Clamp, Mass Spectrometry, Ab Array, Dot Blot, Silver Staining, Electrophoretic Mobility Shift Assay, Activity Assay, Microinjection, MTT Assay, Multiplex Assay, Activation Assay, SPR Assay, Animal Model, Phospho-proteomics, Immunoprecipitation, Luciferase, Sedimentation, Recombinant, Inhibition, Mutagenesis, Transmission Assay, Electron Microscopy, Immunohistochemistry, Immunodepletion, Control, Knock-Out, Conditioned Place Preference, Microscopy, Residue