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     Combing through more than 330 million products and articles...      Welcome to Your Next Discovery   
All (7)
Thermo Fisher
MilliporeSigma
Biogen
Cambridge Bioscience
Santa Cruz
Babco
Biodesign International
 

About 10 products / 27 snippets / 7 images

91 / 100
Bioz Stars
Monoclonal antibody 5F10
Thermo Fisher Scientific / Affinity  
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Techniques
 
Article Snippets
"The monoclonal antibody 5F10 (Affinity Bioreagents, Inc, Golden, Colo.) was used to detect total PMCA immunoreactivity."  (see more)
85 / 100
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Monoclonal antibody 5F10
Santa Cruz  
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Techniques
 
Article Snippets
"Immunoblots have been performed by using anti-PMCA1/4 monoclonal antibody 5F10 (Santa Cruz Biotecnology, INC), or antiACT1 (Sigma)."  (see more)
3+  more images
Fig. 1. Immunolocalization of the PMCA (A) and SERCA (B) pumps in transiently transfected CHO cells. The staining with monoclonal antibodies <t>5F10</t> and IID8, respectively, was revealed by a FITC-conjugated antibody. Immunocytochemistry was performed as described in Materials and methods. Bars, 10 µm.
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siRNA depletion of COPI increases autophagy. (A) siRNA depletion of β′-, β-, and α-COPI (top) and Sec23A and B (bottom) in <t>293/GFP-LC3</t> cells for 48 h. Decreased protein levels of the corresponding subunits is shown by immunoblots. (B) Loss of β′-, α-, and β-COP but not subunits of COPII (Sec 23A and B) increase GFP-LC3-II. In A and B, tubulin was the loading control. (C) <t>GFP-LC3–positive</t> vesicles were detectable in 293/GFP-LC3 cells after siRNA depletion of β′-, α-, and β-COP but only basal levels of GFP-LC3–positive vesicles are observed in control siRNA-depleted cells.
Fig. 2. Western blot analysis of PMCA- and SERCA-overexpressing cells. Lysates from the transiently transfected CHO cells were prepared and separated by SDS–PAGE as described in Materials and methods. Lane 1 corresponds to CHO cells co-transfected with erAEQ/PMCA4 expression plasmids, lane 2 to cells co-transfected with erAEQ/SERCA2b, lane 3 to cells transfected with the empty plasmid and lane 4 to cells transfected with the erAEQ expression vector. The blot was probed with four different monoclonal antibodies: <t>5F10</t> (A), JA9 (B), IID8 (C) and 12CA5 (D).
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Time course of COPI depletion shows that Golgi disperses before AVs are formed, but Golgi dispersal does not cause AV formation. (A) COPI subunits were depleted in <t>293/GFP-LC3</t> cells and analyzed 24, 36, and 48 h after addition of siRNA. Immunoblotting for β′- and α-COP confirms loss of COP subunits. (B) GFP-LC3-I and -II were monitored by immunoblots in parallel lysates using anti-GFP antibodies at the indicated times. (C) After 24 h siRNA treatment, depletion of β′-COP caused morphological changes and a reduction in perinuclear population of GM130. Box indicates enlarged area. (D) Rab1a/b was depleted using siRNAs for 48 h. Lysates were probed with anti-Rab1 to confirm depletion. (E) β′-COP or Rab1a/b were depleted as in D, and analyzed by indirect immunofluorescence using anti-GM130 and GFP fluorescence. In β′-COP panel, the asterisk indicates cells that did not show an accumulation of <t>GFP-LC3–positive</t> AVs.
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85 / 100
Bioz Stars
Anti-rat VCAM-1 mAb 5F10
Biogen  
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Techniques
 
Article Snippets
"The anti-human a4 integrin mAb HP2/1 (mouse IgG1), recognizing rat a4 (34), and the anti-rat VCAM-1 mAb 5F10 (mouse IgG2a) (16) were from Biogen."  (see more)
85 / 100
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Mouse monoclonal antibody 5F10
MilliporeSigma / Sigma Aldrich  
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Article Snippets
"Plasma membrane Ca2+-ATPase (PMCA) was detected with mouse monoclonal antibody 5F10 (10 μg/ ml; Sigma-Aldrich Chemie GmbH, Taufkirchen, Germany) against human erythrocyte PMCA (Borke et .."  (see more)
85 / 100
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Mouse monoclonal anti-PMCA 5F10
Cambridge Bioscience  
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Techniques
 
Article Snippets
".. monoclonal anti-calbindin-D28K (1:2,500 dilution; Sigma, Poole, Dorset, UK), mouse monoclonal anti-PMCA 5F10 (1:1,500 dilution; Cambridge Bioscience, Cambridge, UK) and affinity-purified rabbit polyclonal anti-ECaC1 (1:1,000 .."  (see more)
80 / 100
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Monoclonal anti-(human erythrocyte PMCA) antibody (Clone 5F10)
Cambridge Bioscience  
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Techniques
 
Article Snippets
"Monoclonal anti-(human erythrocyte PMCA) antibody (Clone 5F10) was purchased from Cambridge Bioscience, Cambridge, U.K. Collagenase (Type Ia), anti-(mouse IgG) .."  (see more)
85 / 100
Bioz Stars
Monoclonal antibody (5F10)
Babco Inc  
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Techniques
 
Article Snippets
"Abstract: Results At 1 week, the mean arterial pressure was increased significantly at the cervical artery in the group with aortic constriction (160 6 4 mmHg, compared with 104 6 2 mmHg before constriction), but not at the femoral artery (11 ..."  (see more)
80 / 100
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Anti- 2GPI monoclonal antibody 5F10.F3
Biodesign International  
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Techniques
 
Article Snippets
"Abstract: Lipoprotein(a) [Lp(a)] may be an independent risk factor for coronary artery disease (CAD).. Lp(a) is enriched in platelet activating factor cetylhydrolase (PAF-AH), an enzyme which hydrolyzes and inactivates platelet activating fac ..."  (see more)
88 / 100
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Antirat VCAM-1 MAb 5F10 (IgG2a)
Biogen  
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Techniques
 
Article Snippets
"Abstract: Background: Chronic obstructive uropathy induced by maintained unilateral ureter ligation in the rat is characterized morphologically by interstitial inflammation, interstitial fibrosis, and tubular atrophy.. Infiltrating mononuclea ..."  (see more)
80 / 100
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Anti-VCAM-1 mAb 5F10 (IgG2a)
Biogen  
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Techniques
 
Article Snippets
"Abstract: We characterized the early effects of anti-very late antigen (VLA-4) and its counterligand vascular cell adhesion molecule-1 (VCAM-1) antibody therapy on T cell infiltration and apoptosis in adoptive transfer experimental autoimmune ..."  (see more)