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Image Search Results
Journal: Cell Death Discovery
Article Title: N 6 -methyladenosine (m 6 A) writer METTL5 represses the ferroptosis and antitumor immunity of gastric cancer
doi: 10.1038/s41420-024-02166-1
Figure Lengend Snippet: A SRAMP ( http://www.cuilab.cn/sramp ) demonstrated that there was remarkable m 6 A site on the NRF2 (NFE2L2 gene). B The m 6 A motif on NRF2 was focused on ‘GA’ nucleotides. C NRF2 mRNA expression was tested using RT-PCR. D , E RNA decay analysis was performed to test the half life time (t 1/2 ) of NRF2 mRNA with METTL5 silencing or METTL5 overexpression. F NRF2 protein level was detected in GC cells with METTL5 silencing or METTL5 overexpression. G RIP-PCR was performed to detect the immunoprecipitated NRF2 RNA using anti-m 6 A antibody. RNA samples were extracted from GC cells. Results are evaluated using the two-tailed Student’s t-test. * p < 0.05; ** p < 0.01.
Article Snippet: Then, the cell extract was incubated by Protein A/G magnetic beads (cat. B26101,
Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Over Expression, Immunoprecipitation, Two Tailed Test
Journal: Cell Death Discovery
Article Title: N 6 -methyladenosine (m 6 A) writer METTL5 represses the ferroptosis and antitumor immunity of gastric cancer
doi: 10.1038/s41420-024-02166-1
Figure Lengend Snippet: A , B The NRF2 mRNA was detected using RT-PCR in GC cells (AGS, MKN-45) transfected with siRNA targeting several m 6 A readers. C RIP-PCR was performed in GC cells (AGS, MKN-45) using anti-IGF2BP1/anti-IgG to detect the NRF2 mRNA level in immunoprecipitation. D , E The RIP-PCR was performed in GC cells (AGS, MKN-45) using anti-IGF2BP1/anti-IgG to detect the NRF2 mRNA with METTL5 silencing or overexpression. AGS cell was transfected with sh-METTL5-1/2, and MKN-45 cell was transfected with oe-METTL5. F , G RNA decay analysis revealed the half life time (t 1/2 ) of NRF2 mRNA in GC cells with IGF2BP1 silencing (si-IGF2BP1). H The luciferase reporters were inserted either the wild-type (WT) or mutant (Mut) sequences. I The luciferase activity was detected when co-transfected with NRF2-WT/Mut vectors and IGF2BP1 silencing (si-IGF2BP1). Results are evaluated using the two-tailed Student’s t-test. * p < 0.05; ** p < 0.01.
Article Snippet: Then, the cell extract was incubated by Protein A/G magnetic beads (cat. B26101,
Techniques: Reverse Transcription Polymerase Chain Reaction, Transfection, Immunoprecipitation, Over Expression, Luciferase, Mutagenesis, Activity Assay, Two Tailed Test
Journal: Cell Death Discovery
Article Title: N 6 -methyladenosine (m 6 A) writer METTL5 represses the ferroptosis and antitumor immunity of gastric cancer
doi: 10.1038/s41420-024-02166-1
Figure Lengend Snippet: m 6 A writer METTL5 targets NRF2 to ameliorate GC cells ferroptosis and represses activated PBMCs (CD8 + T cells) antitumor immunity.
Article Snippet: Then, the cell extract was incubated by Protein A/G magnetic beads (cat. B26101,
Techniques: