Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Intratumoral activation of 41BB co-stimulatory signals enhances CD8 T cell expansion and modulates tumor-infiltrating myeloid cells

doi: 10.4049/jimmunol.2000759

Figure Lengend Snippet: 41BBL expression on APCs alters the capacity to prime TILs. TILs from a melanoma patient were cultured with αCD3, autologous tumor cells at a 1:1 ratio, or tumor-lysate pulsed 41BBL APCs at a 1:10 ratio for 72hrs. A and B, Heatmap representing cytokine abundance in supernatants from cell cultures were collected at 72hrs. Numerical values are indicated for each parameter with its respective condition. Cytokine production by TIL stimulated with αCD3 +/− urelumab (A). Cytokines in TIL-tumor co-cultures +/− α41BB or MHC-I blocking antibodies (B). C, 41BBL APCs were generated and then pulsed with autologous tumor lysate in the presence of GMCSF. Pulsed APCs were then seeded in culture wells with or without α41BB. D, Cytokines were measured in the supernatants of TIL-APC co-cultures incubated with α41BB and/or α 41BBL. TIL only condition is the same data from (B); APCs only from (C). Statistics are indicated for cytokines that are higher than both TILs alone and APCs alone conditions. E, Fold change in cytokine induction vs. TIL+APCs in co-cultures from (D). Dotted line represents the basal induction of cytokines in TIL-APC co-cultures. F, TIL proliferation in co-cultures was determined in the final 18hrs of the culture by 3H thymidine incorporation in the presence of soluble α41BB and/or soluble α41BBL. Dotted line represents basal TIL proliferation without additional stimulation. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. Significance was determined by two-tailed t-test or 2-way ANOVA with Dunnett’s multiple comparisons. ND = not detected.

Article Snippet: The H. Lee Moffitt Cancer Center and Research Institute has licensed intellectual property related to the proliferation and expansion of tumor infiltrating lymphocytes (TILs) to Iovance Biotherapeutics.

Techniques: Expressing, Cell Culture, Blocking Assay, Generated, Incubation, Two Tailed Test

Journal: PLoS ONE

Article Title: Deletion of ssnA Attenuates the Pathogenicity of Streptococcus suis and Confers Protection against Serovar 2 Strain Challenge

doi: 10.1371/journal.pone.0169791

Figure Lengend Snippet: (A) Splenocytes from immunized mice were collected at 28 day post-first immunization and lymphocyte proliferation assay was evaluated using a cell proliferation kit with MTT reagent. (B) Production of cytokines in stimulated splenocytes was detected by ELISA. * P < 0.05. Δ, versus the negative control.

Article Snippet: Lymphoproliferation assays were carried out with MTT reagent using a lymphocyte proliferation kit (Promega, USA) following the manufacturer’s protocol.

Techniques: Lymphocyte Proliferation Assay, Enzyme-linked Immunosorbent Assay, Negative Control