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All (28)
MilliporeSigma
Cell Signaling
Genosphere Bio
Santa Cruz
Abnova
ProSci
OriGene
Abcam
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About 63 products / 74 snippets / 5 images

94 / 100
Bioz vStars
Anti-Kindlin3
CancerTools  
Catalog#: 151882
Description
"Investigation of signalling in human leukocytes; diagnostic for LAD-III patients."
93 / 100
Bioz vStars
kindlin3 synthetic peptide
Thermo Fisher Scientific  
Catalog#: PEP0718
Description
"KINDLIN3 Synthetic Peptide for BLOCK Ctrl"
93 / 100
Bioz Stars
FERMT3 Antibody
Proteintech  
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Catalog#: 18131-1-AP
Techniques
Staining, Incubation, Blocking Assay, Immunofluorescence (see more)
Article Snippets
"663 Total internal reflection fluorescence (TIRF) microscopy 664 GFP-Kindlin-3 and mutant expressing .... LFA-1) (1:50 dilution) (Proteintech) was used and .."  (see more)
86 / 100
Bioz Stars
Kindlin-3
Cell Signaling Technology  
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Techniques
 
Article Snippets
".. Danvers, MA, USA); full-length and cleaved caspase 3 (31A1067, Novusbio, Englewood, CO, USA); kindlin-3 (#10459, Cell Signaling, Danvers, MA, USA); anti-rat IgG, Alexa 594-conjugated antibody (ab150160, Abcam, Cambridge, UK); anti-rabbit .."  (see more)
1+  more images
(A) NMR 2D-HSQC shows that the PXN LIM4 domain interacts with the <t>Kindlin-3</t> F0 domain. The HSQC spectra of 100 μM 15N-labeled PXN-LIM4 in the absence (black) and presence of 300 μM Kindlin-3 F0 (red) are shown. (B) NMR 2D-HSQC shows that the LPXN LIM4 domain interacts with the Kindlin-3 F0 domain. The HSQC spectra of 100 μM 15N-labeled LPXN-LIM4 in the absence (black) and presence of 300 μM Kindlin-3 F0 (red) are shown. (C) GST pull-down demonstrates the interaction between flag-Kindlin-3 expressed in HEK293 cells with both GST-PXN and GST-LPXN. (D) The affinity of Kindlin-3 F0 binding to the PXN LIM4 domain (Kd: 117.2 μM) measured by HSQC titration. Titration curves of four representative residues are shown. (E) The affinity of Kindlin-3 F0 binding to the LPXN LIM4 domain (Kd: 192.3 μM) measured by HSQC titration. Titration curves of four representative resonance peaks are shown. (F) The HSQC spectra of 100 μM 15N-labeled PXN-LIM4 in the absence (black) and presence of 300 μM Kindlin-3 F0-G43K/L47E mutant (red) suggest that G43K/L47E mutations block Kindlin-3 F0 binding to PXN-LIM4. (G) The HSQC spectra of 100 μM 15N-labeled LPXN LIM4 domain in the absence (black) and presence of 300 μM Kindlin-3 F0-G43K/L47E mutant (red) suggest that G43K/L47E mutations block Kindlin-3 F0 binding to LPXN-LIM4. Values=mean±S.D. (H) GST pull-down assay demonstrates that Myc-PXN expressed in HEK293 cells interacts with GST-Kindlin-3-F0 by conserved Kindlin-3-F0-G43/L47. (I) GST pull-down assay demonstrates Flag-Kindlin-3, but not Flag-Kindlin-3-G43K/L47E expressed in HEK293 cells, interacts with GST-PXN-LIM4 and GST-LPXN-LIM4. (J) GST pull-down assay demonstrates interactions between both HA-Kindlin-3 (FL, full length) and HA-K3KI (Kindlin-3 mutant, that does not bind to integrin) with PXN/LPXN-LIM4 domain. (K) Quantification of Western blotting shows similar binding of HA-Kindlin-3 (FL, full length) and HA-K3KI with PXN/LPXN-LIM4, n=2.
LBLNG
BIND-A
TITRA
MUTA
BLOCK-A
KEY RESOURCES TABLE
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RECOMB
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(A) A representative schematic of generating process of K3KO and their rescued cells. K3KO cells were generated by CRISPR-cas9 and then re-expressed vector (Vec) (Plvx-ds-red-mono vector), human <t>Kindlin-3</t> (hK3) (Plvx-ds-red-mono-hK3) or human Kindlin-3 (hK3pxn) (Plvx-ds-red-mono-hK3-G43K/L47E), respectively, by lentiviral infection. (B) Flow cytometry analysis demonstrates equal levels of hK3 and hK3pxn expression in K3KO cell lines based on the Ds-Red signal. K3KO cells with hK3 and hK3pxn were analyzed by flow cytometry. Wild-type cells were used as Mock (negative control). (C) Western blot showing equal levels of hK3 and hK3pxn expression in K3KO Raw264.7 cells. The protein levels of Kindlin-3 were detected, using an antibody for mouse Kindlin3 (mK3), human Kindlin-3 (Ds-red- hK3), and GAPDH was used as a loading control. Both, mouse Kindlin-3 (mK3) and human K3 (hK3)-Ds-Red fusion are indicated. (D) Confocal images of Control cells and K3KO cells seeded on fibronectin pre-coated coverslips for 2 hours with or without 20ng/ml C5a treatment. Staining for PXN (green), F-actin (purple, Alexa-Fluor 647-Phalloidin) and DAPI are shown. Scale bar=20μm. (E, F) Quantification of cell area and cell polarity in Control and K3KO cells. Control (without C5a, n=35, and with C5a, n=34) and K3KO (without C5a, n=34, and with C5a n=27).Values=means+SEM. p*<0.05, p***<0.001 based on Student’s t-test. (G) Quantification of protrusions from Figure 2D. Control (without C5a, n=35, with C5a, n=25) and K3KO (without C5a, n=35 and with C5a, n=36).Values=means+SEM. p***<0.001 based on Student’s t-test. (H) Confocal images of K3KO cells expressing WT Kindlin-3 (hK3) or Kindlin-3-PXN /LPXN mutant (hK3pxn) seeded on fibronectin pre-coated coverslips for 2 hours. Staining for PXN (green), F-actin (purple, Alexa-Fluor 647-Phalloidin) and DAPI are shown. Ds-red indicates the presence of hK3 or hK3pxn. Scale bar, 20μm. (I) Confocal images showing actin-rich protrusions of hK3 or hK3pxn cells seeded on fibronectin for 2 hours with or without 20ng/ml C5a. Staining for PXN (green), F-actin (purple, Alexa-Fluor 647-Phalloidin) and DAPI are shown. White arrows indicate cell protrusions. Scale bar=10μm. (J) Quantification of cell area in K3KO cells expressing either WT Kindlin-3 (hK3) or Kindlin-3-PXN/LPXN mutant (hK3pxn). K3KO with hK3 (without C5a, n=55 and with C5a, n=88) and re- hK3pxn mutant (without C5a, n=78, and with C5a, n=113).Values=means+SEM. p**<0.005 and p***<0.001 based on Student’s t-test. (K) Quantification of cell area in K3KO cells expressing either WT Kindlin-3 (hK3) or Kindlin-3-PXN/LPXN mutant (hK3pxn). K3KO with hK3 (without C5a, n=69 and with C5a, n=85) and with hK3pxn (without C5a, n=86, and with C5a, n=96).Values=means+SEM. p***<0.001 based on Student’s t-test. (L) Quantification of the number of protrusions from Figure 2I. K3KO with hK3 (without C5a, n=38, with C5a, n=35) and with hK3pxn (without C5a, n=38 and with C5a, n=35).Values=means+SEM. p**<0.005 and p***<0.001 based on Student’s t-test. (M) Quantification of the number of protrusions from Figure 2I. K3KO with hK3 (without C5a, n=132, with C5a, n=354) and with hK3pxn (without C5a, n=509 and with C5a, n=648).Values=means+SEM. p*<0. 05, p**<0.005 and p***<0.001 based on Student’s t-test.
GENER
CRISPR
PLASMID-P
INFECT
FC
(A) Representative tracks of K3KO Raw 264.7 cells re-expressing <t>Kindlin-3</t> WT (hK3) or Kindlin-3-PXN (hK3pxn) mutant within 24 hours by phase-contrast microscopy time-lapse imaging. The starting point is at the origin, and each point represents the cell position at 25 min intervals. K3KO#1 re-expressed hK3 (n=31) and hK3pxn (n=64), and K3KO#2 re-expressed hK3 (n=71) and hK3pxn (n=57). (B) Bar graphs show the average velocity of cells from Figure 4A. 2.5×SD (standard deviation) was removed from consideration. Values=means+SEM, p*<0.05, p**<0.005 based on Student’s t-test. (C) Representative confocal images of K3KO with hK3 and hK3pxn seeded on fibronectin for 20 min. Membrane was labeled with PKH67 (green), Alexa-Fluor 647-Phalloidin (purple) indicates F-actin, and Ds-red indicates hK3 and hK3pxn expression. White arrows indicate cell blebs. Scale bar, 10μm. (D) Quantification of blebs numbers per cell. K3KO#1 with hK3 (n=75) and hK3pxn (n=75), and K3KO#2 with hK3 (n=46) and hK3pxn (n=45) were analyzed. Values=means+SEM. p***<0.001 based on Student’s t-test. (E) DIC microscopy time-lapse imaging showing the dynamics of cell blebbing in K3KO cells with hK3 or hK3pxn expression at the initial adhesion stage (0–30min) in 2D in three independent experiments. The images were exacted from z-stacks. White arrows and arrowheads indicate cell blebs and filopodia, respectively. Scale bar=10μm. (F) Characterization of cell protrusions and blebbing during adhesion. Bars show the ratios of various protrusions from Figure 4E in three independent experiments. K3KO#1 with hK3 (n=77) and hK3pxn (n=74), and K3KO#2 with hK3 (n=52) and hK3pxn (n=53) were analyzed. (G) DIC microscopy time-lapse imaging showing the dynamics of cell blebbing in K3KO cells with hK3 or hK3pxn expression at the firm adhesion stage (16–18hrs after seeding). Images were etrxacted from z-stacks. White arrows and arrowheads indicate cell blebs and lamellipodia, respectively. (H) Characterization of cell protrusions and blebbing from Figure 4G in three independent experiments. Ratios of various protrusions types are shown. K3KO#1 with hK3 (n=45) and hK3pxn (n=48), and K3KO#2 with hK3 (n=59) and hK3pxn (n=60) were analyzed. (I) Quantification of the relative lifetime of protrusions of hK3 and hK3pxn cells at the firm adhesion stage in Figure 4G. The average interval of each video is 27-second. hK3 (K3KO#1) (Lamellipodia, n=37, filopodia, n=81, and blebs, n=0), and hK3pxn (K3KO#1) (Lamellipodia, n=25, filopodia, n=125, and blebs, n=52). hK3 (K3KO#2) (Lamellipodia, n=0, filopodia, n=17, and blebs, n=78), and hK3pxn (K3KO#2) (Lamellipodia, n=17, filopodia, n=145, and blebs, n=34). Values=means+SEM. Values=means+SEM. p***<0.001 based on Student’s t-test.
EXP
MUTA
MICRO
IMG
SD
×
80 / 100
Bioz Stars
Anti Kindlin-3
Cell Signaling Technology  
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Techniques
 
Article Snippets
".. 1:1000], GADPH [CST #2118S (14C10); 1:1000], Aequorea victoria GFP [Clontech #632381 (JL-8); 1:2000], kindlin-3 [CST #13843S; 1:1000], talin-1 [CST #4021S (C45F1); 1:1000], zyxin (CST #3553S; 1:1000), SLP-76 .."  (see more)
80 / 100
Bioz Stars
Anti Kindlin-3
Cell Signaling Technology  
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Techniques
 
Article Snippets
".. for western blotting in this study were Kindlin-1 (Sigma, SAB4200465, 1:1,000), Kindlin-3 (Cell Signaling, Cat# 13,843, 1:300), TGFβ R1 (Santa Cruz, sc-9048, 1:500), TGFβ R2 (Santa Cruz, sc-400, .."  (see more)
80 / 100
Bioz Stars
Anti Kindlin-3
Genosphere Bio  
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Techniques
 
Article Snippets
"Briefly, slides were blocked, incubated with antibodies directed against Kindlin-3 (Genosphere), Integrins β1, β3 or β5 (Abcam ab58524, Santa Cruz sc-14009, sc-14010) or .."  (see more)
88 / 100
Bioz Stars
Kindlin-3 accumulation
SMAC  
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Techniques
 
Article Snippets
"Stable LFA-1-intercellular adhesion molecule-1 interaction coincides with kindlin-3 accumulation in the SMAC consistent with the role of kindlin-3 in maintaining high affinity LFA-1 .."  (see more)
80 / 100
Bioz Stars
Anti Kindlin‐3
Affinity Biosciences  
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Techniques
 
Article Snippets
"Kindlin‐3 , Affinity Biosciences , DF13558 , 1:1000 (WB), 1:200 (IHC)."  (see more)
80 / 100
Bioz Stars
Anti Kindlin-3
MilliporeSigma / Sigma Aldrich  
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Techniques
 
Article Snippets
".. beta2-integrin (Abcam, MA, USA), GM-CSF receptor beta-common chain (Santa Cruz Biotechnology, CA, USA), kindlin-3 (SigmaAldrich, MO, USA), talin, CD9 (BD Biosciences)."  (see more)
80 / 100
Bioz Stars
Anti Kindlin-3
ProSci  
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Techniques
 
Article Snippets
"The blots were incubated with antibodies to kindlin-1, 14 kindlin-2, 15 kindlin-3 (ProSci, San Diego, CA), or GAPDH (clone 6C5; Millipore) overnight at 4°C, followed .."  (see more)
85 / 100
Bioz Stars
KINDLIN-3 primers
SuperArray Bioscience  
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Techniques
 
Article Snippets
"GAPDH , KINDLIN-2 , and KINDLIN-3 primers were from SuperArray."  (see more)
80 / 100
Bioz Stars
Kindlin-3 mice
Charles River  
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Techniques
 
Article Snippets
"S 485 A kindlin-3 mice were generated in Charles River Laboratories."  (see more)
80 / 100
Bioz Stars
Anti–kindlin-3
Cell Signaling Technology  
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Techniques
 
Article Snippets
".. with mouse anti-GAP DH (M20006; Abmart), rabbit anti–kindlin-1 (ABT422, Millipore), rabbit anti–kindlin-3 (10459S; Cell Signaling), mouse anti–kindlin-2 (clone 3A3.5; Tu et al., 2003; Wu et al., .."  (see more)
94 / 100
Bioz vStars
Anti-Kindlin3
CancerTools  
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Catalog#: 151882
Description
"Investigation of signalling in human leukocytes; diagnostic for LAD-III patients."
93 / 100
Bioz vStars
kindlin3 synthetic peptide
Thermo Fisher Scientific  
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Catalog#: PEP0718
Description
"KINDLIN3 Synthetic Peptide for BLOCK Ctrl"
93 / 100
Bioz vStars
Kindlin-3 Antibody
NSJ Bioreagents  
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Catalog#: R33951
Description
"Additional name(s) for this target protein: Fermitin family homolog 3; FERMT3"
93 / 100
Bioz vStars
Human Kindlin 3
Shanghai Korain Biotech Co Ltd  
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Catalog#: E2775HU
93 / 100
Bioz vStars
Kindlin-3 Antibody
NSJ Bioreagents  
Catalog#: R33951
Description
"Additional name(s) for this target protein: Fermitin family homolog 3; FERMT3"
93 / 100
Bioz vStars
Human Kindlin 3
Shanghai Korain Biotech Co Ltd  
Catalog#: E2775HU