k562 transfectants Search Results


dp2-transfected line k562/b19  (BML Inc)
90
BML Inc dp2-transfected line k562/b19
Expression of DP2 and FcεRI on hPBDMC and LAD2 were examined by flow cytometry. A representative result of dot plot (left) and MFI (Mean Fluorescent Intensity, right) from five to eight independent experiments calculated using WinMDI ver.2.9 software (mean±SEM) are shown. (A) Total expression of DP2 in hPBDMC ( n = 8). (B) Total expression of DP2 in LAD2 ( n = 5). (C) Surface expression of DP2 in hPBDMC ( n = 8). (D) Surface expression of DP2 on LAD2 ( n = 5). (E) Surface expression of FcεRI on hPBDMC ( n = 15–21). (F) Surface expression of FcεRI ( n = 5) on LAD2. (G) Surface expression of DP2 on DP2 transfectant, <t>K562/B19</t> ( n = 3) was examined as a control. In dot plot, the percentage of positive cells was shown inside, and gray, black and red dots represent unstained, stained with isotype control and specific antibody, respectively. † p<0.05, †† p<0.01, ††† p<0.001 compared with unstained; **p<0.01, ***p<0.001 compared with isotype control by repeated measures ANOVA followed by the Tukey post-test.
Dp2 Transfected Line K562/B19, supplied by BML Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/k562+transfectants/dp2+transfected+line+k562+b19/pmc04182489-46-2-16
Average 90 stars, based on 1 article reviews
dp2-transfected line k562/b19 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


Expression of DP2 and FcεRI on hPBDMC and LAD2 were examined by flow cytometry. A representative result of dot plot (left) and MFI (Mean Fluorescent Intensity, right) from five to eight independent experiments calculated using WinMDI ver.2.9 software (mean±SEM) are shown. (A) Total expression of DP2 in hPBDMC ( n = 8). (B) Total expression of DP2 in LAD2 ( n = 5). (C) Surface expression of DP2 in hPBDMC ( n = 8). (D) Surface expression of DP2 on LAD2 ( n = 5). (E) Surface expression of FcεRI on hPBDMC ( n = 15–21). (F) Surface expression of FcεRI ( n = 5) on LAD2. (G) Surface expression of DP2 on DP2 transfectant, K562/B19 ( n = 3) was examined as a control. In dot plot, the percentage of positive cells was shown inside, and gray, black and red dots represent unstained, stained with isotype control and specific antibody, respectively. † p<0.05, †† p<0.01, ††† p<0.001 compared with unstained; **p<0.01, ***p<0.001 compared with isotype control by repeated measures ANOVA followed by the Tukey post-test.

Journal: PLoS ONE

Article Title: Expression of DP2 (CRTh2), a Prostaglandin D 2 Receptor, in Human Mast Cells

doi: 10.1371/journal.pone.0108595

Figure Lengend Snippet: Expression of DP2 and FcεRI on hPBDMC and LAD2 were examined by flow cytometry. A representative result of dot plot (left) and MFI (Mean Fluorescent Intensity, right) from five to eight independent experiments calculated using WinMDI ver.2.9 software (mean±SEM) are shown. (A) Total expression of DP2 in hPBDMC ( n = 8). (B) Total expression of DP2 in LAD2 ( n = 5). (C) Surface expression of DP2 in hPBDMC ( n = 8). (D) Surface expression of DP2 on LAD2 ( n = 5). (E) Surface expression of FcεRI on hPBDMC ( n = 15–21). (F) Surface expression of FcεRI ( n = 5) on LAD2. (G) Surface expression of DP2 on DP2 transfectant, K562/B19 ( n = 3) was examined as a control. In dot plot, the percentage of positive cells was shown inside, and gray, black and red dots represent unstained, stained with isotype control and specific antibody, respectively. † p<0.05, †† p<0.01, ††† p<0.001 compared with unstained; **p<0.01, ***p<0.001 compared with isotype control by repeated measures ANOVA followed by the Tukey post-test.

Article Snippet: The DP2-transfected line K562/B19 and its control line K562/neo was generously provided by Dr. K. Nagata (BML, Inc., Saitama, Japan) and cultured as previously described .

Techniques: Expressing, Flow Cytometry, Software, Transfection, Staining

Expression of DP2 on LAD2 and hPBDMC were examined with ImageStream after staining live cells for surface expression (A, C) or with fixed and permeabilized cells for total expression (B, D). (A, C) After surface staining, DP2 signals were detected from inside MC (open triangle) rather than on the surface. (B, D) Intracellular punctate staining for DP2 (arrow) was observed in fixed and permeabilized MC before staining. (E, F) K562/B19 (DP2 transfectant) was used as a control for surface expression of DP2 (closed triangle). Representative images of cells stained with isotype matched control Ab (left) and DP2 positive cells (right) from three independent experiments are shown.

Journal: PLoS ONE

Article Title: Expression of DP2 (CRTh2), a Prostaglandin D 2 Receptor, in Human Mast Cells

doi: 10.1371/journal.pone.0108595

Figure Lengend Snippet: Expression of DP2 on LAD2 and hPBDMC were examined with ImageStream after staining live cells for surface expression (A, C) or with fixed and permeabilized cells for total expression (B, D). (A, C) After surface staining, DP2 signals were detected from inside MC (open triangle) rather than on the surface. (B, D) Intracellular punctate staining for DP2 (arrow) was observed in fixed and permeabilized MC before staining. (E, F) K562/B19 (DP2 transfectant) was used as a control for surface expression of DP2 (closed triangle). Representative images of cells stained with isotype matched control Ab (left) and DP2 positive cells (right) from three independent experiments are shown.

Article Snippet: The DP2-transfected line K562/B19 and its control line K562/neo was generously provided by Dr. K. Nagata (BML, Inc., Saitama, Japan) and cultured as previously described .

Techniques: Expressing, Staining, Transfection