Journal: PeerJ

Article Title: Influence of CNTRENE ® C100LM carbon nanotube material on the growth and regulation of Escherichia coli

doi: 10.7717/peerj.3721

Figure Lengend Snippet: Controls cells were grown in medium without CNT treatment. E. coli K12 were grown in the presence of 0–1.05 µg/ml pristine or aged CNTRENE C100LM. (A) E. coli exposed to pristine CNTRENE C100LM and grown in LB at a pH of 7. (B) E. coli exposed to aged CNTRENE C100LM and grown in LB at pH 7. (C) E. coli exposed to pristine CNTRENE C100LM and grown in M9 medium at a pH of 7. (D) E. coli exposed to aged CNTRENE C100LM and grown in M9 medium at pH 7. (E) E. coli exposed to pristine CNTRENE C100LM and grown in LB at a pH of 5. (F) E. coli exposed to aged CNTRENE C100LM and grown in LB at pH 5. Error bars have been omitted for clarity.

Article Snippet: Escherichia coli K12 strain SMG 123 (ATCC PTA-7555) was grown in lysogeny broth (LB) or M9 minimal salts medium with the addition of 1 mM thiamine and 2% glucose (hereafter M9 medium) at 37 °C and 200 rpm in a Thermo Scientific MaxQ 400 incubator unless stated otherwise.

Techniques:

Journal: PeerJ

Article Title: Influence of CNTRENE ® C100LM carbon nanotube material on the growth and regulation of Escherichia coli

doi: 10.7717/peerj.3721

Figure Lengend Snippet: Treatment with either pristine (dark gray) or artificially aged (light gray) CNTRENE C100LM. Controls (unfilled bar) were grown in medium without CNTRENE C100LM. (A) E. coli grown in LB at pH 7. For pristine CNTRENE C100LM treatment t d ranged from 20.3 min–25.3 min with an average of 22. 4 min (±1.2 min). Aged CNTRENE C100LM treatment t d ranged from 20.1 min–24.7 min with an average of 22.3 min (±1.1 min). Untreated cells had a t d of 22.7 min (±0.8 min). (B) E. coli grown in M9 medium. For pristine CNTRENE C100LM treatment t d ranged from 54.5 min –65.4 min with an average of 60.4 min (±2.3 min). Aged CNTRENE C100LM treatment t d ranged from 59.0 min –61.6 min, with an average of 59.9 (±0.7 min). Untreated cells had a t d of 60.7 min (±0.7 min). (C) E. coli grown in LB at pH 5. For pristine CNTRENE C100LM treatment t d ranged from 29.1 min –31.9 min with an average of 30.3 min (±0.8 min). Aged CNTRENE C100LM treatment t d ranged from 27.3 min –31.1 min, with an average of 29.6 (±0.9 min). Untreated cells had a td of 30.3 min (±0.5 min). For all treatment groups (pristine or aged CNTRENE C100LM exposure) doubling times mirrored that of the untreated control, regardless of medium or pH used.

Article Snippet: Escherichia coli K12 strain SMG 123 (ATCC PTA-7555) was grown in lysogeny broth (LB) or M9 minimal salts medium with the addition of 1 mM thiamine and 2% glucose (hereafter M9 medium) at 37 °C and 200 rpm in a Thermo Scientific MaxQ 400 incubator unless stated otherwise.

Techniques: Control

Journal: PeerJ

Article Title: Influence of CNTRENE ® C100LM carbon nanotube material on the growth and regulation of Escherichia coli

doi: 10.7717/peerj.3721

Figure Lengend Snippet: CNTRENE C100LM exposure in E. coli K12 grown in LB pH 7. Data were determined to be Gaussian by a D’Agostino-Pearson normality test. Differences were not significant between groups as determined by one-way ANOVA ( p = 0.2138, n = 9).

Article Snippet: Escherichia coli K12 strain SMG 123 (ATCC PTA-7555) was grown in lysogeny broth (LB) or M9 minimal salts medium with the addition of 1 mM thiamine and 2% glucose (hereafter M9 medium) at 37 °C and 200 rpm in a Thermo Scientific MaxQ 400 incubator unless stated otherwise.

Techniques:

Journal: bioRxiv

Article Title: DNA glycosylase NEIL2 prevents Fusobacterium -mediated inflammation and DNA damage in colonic epithelial cells

doi: 10.1101/2020.06.11.147454

Figure Lengend Snippet: (A-D) Human colonic EDMs were infected with Fn at moi 100 for 24 h. The RNA from EDMs was used for qRT-PCR to determine the expression of genes involved in base excision repair, mismatch repair and for non-homologous end joining (NHEJ). (A) Schematic showing the experimental design. (B) The level of BER transcripts, NEIL1, NEIL2, NTH1, OGG1, (C) The level of MMR transcripts, MLH1, MLH3, MSH2, MSH6, PMS2, (D) The transcript level of NHEJ marker Ku70 were determined by qRT-PCR. (E-F) Human colonic EDMs were infected with commensal E. coli -K12 strain (E), or pathogenic IBD-associated adherent invasive E. coli LF-82 (F) to determine the expression level of NEIL2 following infection. In (B-F), the expression level of the transcripts was normalized to the housekeeping gene (18srRNA), and the normalized expression value was compared with the respective uninfected control cells. Data represent the mean ± SEM of three separate experiments. * indicates p≤0.05, and ** indicates p≤0.01 as calculated by the unpaired two-tailed student’s t-test.

Article Snippet: Escherichia coli K12 strain DH10B , (ATCC-PTA¬5105), was cultured on L.B. agar and L.B. broth and used to infect EDM at moi of 100.

Techniques: Infection, Quantitative RT-PCR, Expressing, Non-Homologous End Joining, Marker, Control, Two Tailed Test

Journal: bioRxiv

Article Title: DNA glycosylase NEIL2 prevents Fusobacterium -mediated inflammation and DNA damage in colonic epithelial cells

doi: 10.1101/2020.06.11.147454

Figure Lengend Snippet: (A) APC Min /+ EDMs derived from the uninvolved region of the colon were infected with different microbes; commensal E. coli K12, IBD-associated adherent-invasive E.coli LF82 and colon cancer-associated pathogens (NC101, H. pylori and Fn ). The supernatants were collected from the uninfected and infected EDMs done in the same experiments and assessed for oxidative DNA damage (right). Data represent the mean ± SEM of three separate experiments. * indicates p≤0.05, ** indicates p≤0.01 as assayed by student’s t-test. (B) The relative level of the oxidized bases produced by each microbe was compared with uninfected cells, which is considered as 1. The relative production of the oxidized base was compared between different microbes

Article Snippet: Escherichia coli K12 strain DH10B , (ATCC-PTA¬5105), was cultured on L.B. agar and L.B. broth and used to infect EDM at moi of 100.

Techniques: Derivative Assay, Infection, Produced