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All (18)
Cayman
Selleck Chemicals
Bio-Techne
MilliporeSigma
Croda International
MedChemExpress
Santa Cruz
Tocris
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About 27 products / 64 snippets / 15 images

94 / 100
Bioz vStars
JTE 013
Aladdin Scientific  
Catalog#: J286613-100MG
Description
"JTE-013 has been used in in vitro blood−brain barrier (BBB) and blood−tumor barrier (BTB) assays."
93 / 100
Bioz Stars
JTE 013
Bio-Techne  
Catalog#: 2392
Techniques
 
Article Snippets
" #14126. Torin2 (10nM) , Cayman Chemical , Cat #14185. INK128 (100nM) , Cayman Chemical , Cat #11811. JTE013 (1μM) , Tocris , Cat #2392. SKI-II (0.8μM) , Echelon , Cat #B-0024. NSC26722 (100μM) , Tocris , Cat #2161. BYL719 (1μM) , Cayman Chemical , Cat #16986. BKM"  (see more)
93 / 100
Bioz Stars
JTE 013
Bio-Techne  
star_border
Catalog#: 2392
Techniques
 
Article Snippets
" #14126. Torin2 (10nM) , Cayman Chemical , Cat #14185. INK128 (100nM) , Cayman Chemical , Cat #11811. JTE013 (1μM) , Tocris , Cat #2392. SKI-II (0.8μM) , Echelon , Cat #B-0024. NSC26722 (100μM) , Tocris , Cat #2161. BYL719 (1μM) , Cayman Chemical , Cat #16986. BKM"  (see more)
94 / 100
Bioz Stars
JTE-013
MedChemExpress  
star_border
Catalog#: HY-100675
Techniques
 
Article Snippets
". 65373S, USA), Phospho-Akt (Ser473) (D9E) XP® Rabbit Monoclonal Antibody (CST, Item No. 4060 T, USA), Phospho-mTOR (Ser2448) (D9C2) XP Rabbit Monoclonal Antibody (CST, Item No. 5536 T, USA), JTE-013 (Med Chem Express, Item No. HY-100675, USA) and CBA: Glycochenodeoxycholic Acid (Med Chem Express, Item No. 48305, USA) (concentration: 100umol/L). Cells were washed 2–3 times with PBS, added with app"  (see more)
11+  more images
Inhibition of S1PR2 impairs OE regeneration and functional recovery of the olfactory system after injury (A) H&E staining of the OE obtained from control- and <t>JTE013-treated</t> 2-month-old C57BL/6 mice at 0, 5, and 14 dpl. (B) Quantitative analysis of the thickness of OE as shown in (A) (n = 7 sections from 4 mice per group). (C) Percentage of mice that found cookies over total observed control-treated (n = 8 mice) or JTE013-treated 2-month-old C57BL/6 mice (n = 7 mice) in the buried pellet test at 14 dpl (Student’s t test). (D) Quantitative analysis of the time to locate food in control-treated (n = 8 mice) or JTE013-treated 2-month-old C57BL/6 mice (n = 7 mice) at 14 dpl in the buried pellet test (Student’s t test). (E and F) Double immunostaining of Ki67 (green) and p63 (red) (E) or PH3 (green) and K5 (red) (F) in the OE of control- and JTE013-treated 2-month-old C57BL/6 mice at 0, 3, and 5 dpl. (G and H) Quantitative analysis of the percentage of both Ki67 + and p63 + cells (G) or PH3 + and K5 + (H) cells over p63 + or K5 + HBCs as shown in (E) and (F) (n = 10 sections from 4 mice per group). (I) Double immunostaining of GAP43 (green) and OMP (red) in the OE of control- and JTE013-treated 2-month-old C57BL/6 mice at 0, 7, and 14 dpl. (J and K) Quantitative analysis of the density of GAP43 + (J) or OMP + (K) cells at 0, 3, 7, and 14 dpl (n = 6 sections from 4 mice per group). Images of selected regions are shown at higher magnification. Data are mean ± SEM, two-way ANOVA with Bonferroni’s post tests unless otherwise indicated, compared with control group; ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001. Scale bars, 20 μm.
INHIB
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Endothelial extrusion uses the S1P-S1P2-Rho pathway. A) Quantification of sphingosine kinase (30μM) or JTE-013 inhibitor treated monolayers. B) Quantification of <t>Y27632</t> (50μM) treated monolayers. Data in A and B was analyzed using a 3×2 contingency table and statistical significance measured by a Fisher′s Exact test. C-F) Cells treated with DMSO, Y-27632, sphingosine kinase inhibitor, or S1P2 antagonist (JTE-013) and immunostained for actin (red), active caspase-3 (green), and nuclei (DAPI, blue). Note that only DMSO extrudes by extruded efficient actin ring closure.
( A ) Schematic diagram of S1P biosynthetic cascade enzymes with the fold changes (FGF21KO-preHCC versus WT-preHCC mice). ND, no detection; NC, no change; red, >2-fold up-regulated; blue, <2-fold down-regulated. ( B ) The S1P levels and protein levels of phosphorylated SPHK1 in liver issues from WT/FGF21KO-preHCC mice compared to that from WT/FGF21KO mice. ( C ) The protein levels of phosphorylated YAP and YAP in WT/FGF21KO-preHCC mice. ( D ) S1P content in FL83B/FL83B-FGF21KD cells and Hepal-6/Hepal-6-FGF21KD cells challenged with PA and treated with rhFGF21. ( E ) The protein levels of phosphorylated SPHK1 in FL83B/FL83B-FGF21KD cells and Hepal-6/Hepal-6-FGF21KD cells challenged with PA and treated with rhFGF21. ( F ) The protein levels of phosphorylated YAP in Hepal-6/Hepal-6-FGF21KD cells challenged with PA or PA + S1P and treated with SKI-II, an inhibitor of SPHK1. ( G ) The protein levels of phosphorylated YAP in Hepal-6/Hepal-6-FGF21KD cells challenged with S1P and treated with inhibitors of S1P receptors <t>(JTE013</t> for S1PR2 and W146 for S1PR1). ( H ) Colony-forming assay to detect the number of cell colonies in Hepal-6 cells challenged with S1P and treated with JTE013 or verteporfin, an inhibitor of YAP. ( I ) Schematic diagram for JTE013 treatment in the MASH-HCC mice with WSHFD diets. ( J ) The protein levels of phosphorylated YAP in MASH-HCC mice with JTE013 treatment compared to vehicle controls. 21KO, FGF21KO. * P < 0.05, ** P < 0.01, and *** P < 0.001.
(A) Representative confocal microscope images showing the Ca 2+ ‐derived fluorescence in SH‐SY5Y cells treated for 15 min with 3 μ m Aβ 42 O in the absence or presence of 100 n m S1P, following 30 min of pre‐treatment with the S1PR antagonists W146 (S1P 1 ) at 10 μ m , <t>JTE013</t> (S1P 2 ) at 1 μ m , CAY10444 (S1P 3 ) at 5 μ m , VPC23019 (S1P 1 and S1P 3 ) at 1 μ m and CYM50358 (S1P 4 ) at 1 μ m . Cells were then loaded with the Fluo‐4 AM probe. (B) Semi‐quantitative analysis of the intracellular Ca 2+ ‐derived fluorescence referring to panel (A). (C) MTT reduction in SH‐SY5Y cells treated for 24h with 3 μ m Aβ 42 O in the absence or presence of 100 n m S1P, following 30 min of pre‐treatment with W146 at 10 μ m , CAY10444 at 5 μ m , CYM50358 at 1 μ m and with all the antagonists together at the previously reported concentrations. (D) Representative confocal microscope images showing the Ca 2+ ‐derived fluorescence in SH‐SY5Y cells treated for 15 min with 3 μ m Aβ 42 O in the absence or presence of 100 n m S1P or the S1PR agonists SEW2871 (S1P 1 ) at 10 μ m and CYM50308 (S1P 4 ) at 1 μ m, and both agonists together at the previously reported concentrations. Cells were then loaded with the Fluo‐4 AM probe. (E) Semi‐quantitative analysis of the intracellular Ca 2+ ‐derived fluorescence referring to panel (D). In all panels, data are expressed as the percentage of the value for untreated cells. A total of 80–120 cells (A–B, D–E) and 200 000–250 000 cells (C) were analysed per condition. Experimental errors are SEM ( n = 3). Samples were analysed by one‐way ANOVA followed by Bonferroni's multiple‐comparison test relative to untreated cells (** P < 0.01, *** P < 0.001), to cells treated with Aβ 42 O alone (°° P < 0.01, °°° P < 0.001), and to cells treated with Aβ 42 O + S1P in the absence of S1PR antagonists ( § P < 0.05, §§ P < 0.01). Scale bars, 30 μ m .
MICRO
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94 / 100
Bioz Stars
JTE 013
Selleck Chemicals  
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Catalog#: S7182-10MG
Techniques
RNA Sequencing, Cell Culture, Transfection, Western Blot, Modification (see more)
Article Snippets
".. ATP (5 mM; Beyotime, China) for 30 min. For the S1PR2 agonist and inhibitor experiments, JTE- 013 (Selleck) or CYM-5520 (Selleck) was added to macrophages simultaneously with LPS for 3 h or 6 .."  (see more)
80 / 100
Bioz Stars
JTE 013
Selleck Chemicals  
star_border
Techniques
 
Article Snippets
".. Then, the purified cells were incubated with fresh medium containing 1 μM of JTE 013 (antagonist for S1PR2, CAS 383150-41-2, Selleck, America) or 1 μM of CYM-5520 (agonist for S1PR2, CAS .."  (see more)
91 / 100
Bioz Stars
JTE‐013
MilliporeSigma / Sigma Aldrich  
star_border
Techniques
 
Article Snippets
"Inhibitors VPC‐23019 and rapamycin were procured from Cayman Chemical Company (Ann Arbor, MI, USA), while JTE‐013 was purchased from Sigma‐Aldrich."  (see more)
80 / 100
Bioz Stars
JTE 013 (S7182)
Selleck Chemicals  
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Techniques
 
Article Snippets
"PF-543 (S7177), Opaganib (S7174), Siponimod (S7179) and JTE 013 (S7182) were purchased from Selleck."  (see more)
88 / 100
Bioz Stars
Anti JTE 013
Santa Cruz  
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Techniques
 
Article Snippets
"PKC- , PKC- I, mouse-anti-VEGFR-2 antibody, S1P1–5 antibodies, and JTE 013 were from Santa Cruz Biotechnology (Santa Cruz, CA) or Cayman Chemical (Ann Arbor, MI)."  (see more)
92 / 100
Bioz Stars
S1P2 JTE‐013 antagonist
Cayman  
star_border
Techniques
 
Article Snippets
"The S1P2 JTE‐013 antagonist (Cayman Chemical) was prepared as a 2‐mM solution in ethanol and then further diluted in cell culture medium."  (see more)
80 / 100
Bioz Stars
IQA15041
biosynth carbosynth  
star_border
Catalog#: IQA15041
Techniques
 
Article Snippets
"JTE-013 , Cayman Chemical , 10009458."
80 / 100
Bioz Stars
xwa75693
biosynth carbosynth  
star_border
Catalog#: XWA75693
Techniques
 
Article Snippets
"JTE-013 , Cayman Chemical , 10009458."
80 / 100
Bioz Stars
JTE013
Axon Medchem  
star_border
Techniques
 
Article Snippets
".. (no. S5696, Sigma-Aldrich) at 15 μM, W146 (no. 10009109, Cayman Chemical) at 10 μM, JTE013 (no. 1866, Axon Medchem) at 10 μM, and SSO at 50 μM to treat cells. .."  (see more)
80 / 100
Bioz Stars
JTE013
Croda / Avanti Polar Lipids  
star_border
Techniques
 
Article Snippets
".. antagonists W146 (Avanti Polar Lipids, Alabaster, AL, USA) at 10 μ m (S1P 1 ), JTE013 (Avanti Polar Lipids) at 1 μ m (S1P 2 ), CAY10444 (Cayman Chemical, Ann Arbor, MI, USA) .."  (see more)
MICRO
DER-A
FLUOR
COMP
93 / 100
Bioz Stars
JTE 013
Bio-Techne corporation / Tocris  
star_border
Catalog#: 2392
Techniques
Cell Culture, Recombinant, Activity Assay, Migration (see more)
Article Snippets
".. have effects on endothelial cells (2), or the S1PR2 receptor blocker JTE013 or the S1PR1 and 3 receptor blocker VPC23019 (Tocris Bioscience, Bristol, United Kindom) for 24 h. To evaluate .."  (see more)
94 / 100
Bioz vStars
JTE 013
Aladdin Scientific  
star_border
Catalog#: J286613-100MG
Description
"JTE-013 has been used in in vitro blood−brain barrier (BBB) and blood−tumor barrier (BTB) assays."
94 / 100
Bioz Stars
JTE-013
MedChemExpress  
Catalog#: HY-100675
Techniques
 
Article Snippets
". 65373S, USA), Phospho-Akt (Ser473) (D9E) XP® Rabbit Monoclonal Antibody (CST, Item No. 4060 T, USA), Phospho-mTOR (Ser2448) (D9C2) XP Rabbit Monoclonal Antibody (CST, Item No. 5536 T, USA), JTE-013 (Med Chem Express, Item No. HY-100675, USA) and CBA: Glycochenodeoxycholic Acid (Med Chem Express, Item No. 48305, USA) (concentration: 100umol/L). Cells were washed 2–3 times with PBS, added with app"  (see more)
93 / 100
Bioz Stars
JTE 013
Bio-Techne corporation / Tocris  
Catalog#: 2392
Techniques
Cell Culture, Recombinant, Activity Assay, Migration (see more)
Article Snippets
".. have effects on endothelial cells (2), or the S1PR2 receptor blocker JTE013 or the S1PR1 and 3 receptor blocker VPC23019 (Tocris Bioscience, Bristol, United Kindom) for 24 h. To evaluate .."  (see more)