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Image Search Results
Journal: Gut microbes
Article Title: Gut-derived lactic acid enhances tryptophan to 5-hydroxytryptamine in regulation of anxiety via Akkermansia muciniphila .
doi: 10.1080/19490976.2024.2447834
Figure Lengend Snippet: Figure 5. Lactate and A. muciniphila regulate the directional shift of tryptophan metabolism. (a) Experimental design protocol for the antibiotic reduction and gavage model in mice (n = 6). (b) Comparative analysis of gut microbial composition at the genus level across different groups. (c) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the mouse colon. (d) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. (e) Measurement of 5-HT levels in the mouse colon and serum. (f) Western blot analysis showing the expression of key enzymes involved in tryptophan metabolism in the colon of germ-free mouse (n = 4). (g) Quantitative RT-PCR analysis of Ido1 and Tph1 in germ-free mouse colon, normalized to 18S rRNA. (h) Measurement of 5-HT levels in the germ-free mouse colon and serum. (i) Concentration of SCFAs in the supernatant of BHI medium supplemented with 60% sodium lactate, with or without A. muciniphila. (j) Total movement distance and activity time of mice in open field. (k) Quantitative RT-PCR analysis of Ido1 and Tph1 in mouse colon, normalized to 18S rRNA. Heat-inactivation A. muciniphila : HI-Akk, Propionic acid: PA. (l) Measurement of 5-HT levels in the mouse colon and serum. Data are presented as mean ± SD, with significance indicated by *p < 0.05; **p < 0.01; ***p < 0.001; ns, no significance.
Article Snippet: Akkermansia muciniphila (A. muciniphila, ATCC BAA-835) was anaerobically cultured in
Techniques: Western Blot, Expressing, Quantitative RT-PCR, Concentration Assay, Activity Assay