Journal: EBioMedicine

Article Title: Radiotherapy Upregulates Programmed Death Ligand-1 through the Pathways Downstream of Epidermal Growth Factor Receptor in Glioma

doi: 10.1016/j.ebiom.2018.01.027

Figure Lengend Snippet: The effect of PD-L1 blockade on the suppressing ability of tumor cells for CD8 + T cell proliferation. Cell lines (U87 and U251) were treated with RT (5 Gy), PD-L1 Ab (20 μg/mL), RT + PD-L1 Ab, AG490 (10 μM), RT + AG490, or RT + AG490 + PD-L1 Ab, and then co-cultured with CD8 + T cells. At 48 h, CD8 + T cell proliferation was evaluated by FCM. Non-specific Stimulation: anti-CD3/CD28 beads. Representative images and quantitative data are shown. Each column is shown as the means of three separate experiments; bars, SD. Student's t -test; *** P < 0.001. ANOVA; ns, nonsignificant.

Article Snippet: CD8 + T cell immunomagnetic beads positive selection kit was purchased from Stem-Cell Tech (USA).

Techniques: Cell Culture

Journal: EBioMedicine

Article Title: Radiotherapy Upregulates Programmed Death Ligand-1 through the Pathways Downstream of Epidermal Growth Factor Receptor in Glioma

doi: 10.1016/j.ebiom.2018.01.027

Figure Lengend Snippet: The effect of PD-L1 blockade on the cytotoxicity of CD8 + T cells against glioma cells. Cell lines (U87 and U251) were treated with RT (5 Gy), PD-L1 Ab (20 μg/mL), RT + PD-L1 Ab, AG490 (10 μM), RT + AG490, or RT + AG490 + PD-L1 Ab, and then co-cultured with CD8 + T cells. At 48 h, tumor cell apoptosis was evaluated by FCM. Representative images and quantitative data are shown. Each column is shown as the means of three separate experiments; bars, SD. Student's t-test; *** P < 0.001. ANOVA; ns, nonsignificant.

Article Snippet: CD8 + T cell immunomagnetic beads positive selection kit was purchased from Stem-Cell Tech (USA).

Techniques: Cell Culture