Journal: Histochemistry and Cell Biology

Article Title: Plasma cells, plasmablasts, and AID + /CD30 + B lymphoblasts inside and outside germinal centres: details of the basal light zone and the outer zone in human palatine tonsils

doi: 10.1007/s00418-020-01861-1

Figure Lengend Snippet: Antibodies used

Article Snippet: " , IgE , Acris Antibodies, Herford, Germany, No. DP022.

Techniques:

Journal: Diagnostics

Article Title: The Humanised NPY-mRFP RBL Reporter Cell Line Is a Fast and Inexpensive Tool for Detection of Allergen-Specific IgE in Human Sera

doi: 10.3390/diagnostics12092063

Figure Lengend Snippet: Time course of huNPY-mRFP sensitised with serum of a grass pollen allergic donor and stimulated with a polyclonal antibody against human IgE or a H. lanatus pollen extract, both at 1 μg/mL. Supernatants were collected at the indicated time points and the released fluorescence was measured. Shown is the mean fluorescence ± s.e.m. from three independent experiments, each performed in triplicate. Statistical analysis was performed using two-way ANOVA followed by Tukey multiple comparison test. **** indicates p ≤ 0.0001.

Article Snippet: Using phenol red-free medium (Thermo Scientific Fisher), 65 μL of each of the following conditions was added to the appropriate wells followed by incubation for 45–50 min at 37 °C in a humidified 5% CO 2 incubator: (1) negative control (cells unstimulated); (2) three positive controls 10 μg/mL Concanavalin A (Merck), 1 μg/mL polyclonal goat anti-human IgE antibody (Merck), E. granulosus cyst fluid (10 µg/mL); (3) the recombinant antigens EgEF-1β (0.01–1 µg/mL), EgEF-1δ (0.01–1 µg/mL).

Techniques: Fluorescence

Journal: Diagnostics

Article Title: The Humanised NPY-mRFP RBL Reporter Cell Line Is a Fast and Inexpensive Tool for Detection of Allergen-Specific IgE in Human Sera

doi: 10.3390/diagnostics12092063

Figure Lengend Snippet: Dose-dependent activation of huNPY-mRFP cells upon 45 min exposure to polyclonal anti-human IgE antibody or H. lanatus pollen extract after overnight sensitisation with grass pollen allergic donor serum diluted 1:50. The mean fluorescence ± s.e.m. from three independent experiments, each performed in triplicate is shown. Statistical analysis was performed using ordinary one-way ANOVA followed by Dunnett multiple comparison test. The obtained p values are shown using * for p ≤ 0.05 and **** for p ≤ 0.0001.

Article Snippet: Using phenol red-free medium (Thermo Scientific Fisher), 65 μL of each of the following conditions was added to the appropriate wells followed by incubation for 45–50 min at 37 °C in a humidified 5% CO 2 incubator: (1) negative control (cells unstimulated); (2) three positive controls 10 μg/mL Concanavalin A (Merck), 1 μg/mL polyclonal goat anti-human IgE antibody (Merck), E. granulosus cyst fluid (10 µg/mL); (3) the recombinant antigens EgEF-1β (0.01–1 µg/mL), EgEF-1δ (0.01–1 µg/mL).

Techniques: Activation Assay, Fluorescence