Journal: Biomedicines

Article Title: Neuroprotective Effects of Human-Induced Pluripotent Stem Cell-Derived Mesenchymal Stem Cell Extracellular Vesicles in Ischemic Stroke Models

doi: 10.3390/biomedicines11092550

Figure Lengend Snippet: Characterization of hiPS-MSCs derived extracellular vesicles (hiPS-MSCs-EV) ( A ). Flowchart of hiPS-MSC-EVs isolation from the culture medium. ( B ). Morphology of hiPS-MSC-EVs under transmission electron microscopy. ( C ). Representative bioanalyzer profile of the RNA contained in hiPS-MSC-EVs. ( D ). Western blotting analysis of exosomal positive markers CD63, CD9, and CD81 and negative marker Calnexin in MSC lysate and hiPS-MSC-EVs. Tubulin was used as the loading control. ( E ). Flow cytometry analysis of positive marker CD63 expression after binding to CD9 and CD81 antibody-coated beads. The solid gray line represents cells that were unstained. while solid pink line represents the experimental samples. ( F ). Particle size distribution of iPSC-MSC-EVs measured using a flow nanoanalyzer.

Article Snippet: The cells were incubated with primary antibodies from the human MSC analysis kit (Cat#562245, BD Biosciences, San Jose, CA, USA) to identify the characteristic human MSC surface markers.

Techniques: Derivative Assay, Isolation, Transmission Assay, Electron Microscopy, Western Blot, Marker, Flow Cytometry, Expressing, Binding Assay